Disinfection efficacy and mechanism of olanexidine gluconate against norovirus

Imai, Kazutami; Tanaka, Makoto; Miyoshi, Seiji; Murakami, Ren; Hagi, Akifumi; Yamagawa, Sachi; Sano, Daisuke

doi:10.1016/j.ajic.2021.11.020

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…We performed a killing assay using a 5 min exposure in planktonic cells to evaluate the antimicrobial activity of the CPC/CHA combination and concluded a concentration that allowed this combination to achieve rapid killing of microorganisms in 5 min. Moreover, considering the proteins in the skin and mucosa surface may affect antimicrobial activity, BSA was employed to simulate the disinfection environment [ 39 ]. The results show that CPC and CHA have stronger synergy in the rapid killing of gram-negative bacteria and fungi, as the MBC 5min against E. coli , S. typhimurium , P. aeruginosa , C. lusitaniae , and A. niger in combination was only 1/8–1/4 as they were used alone, while this ratio in MIC was 1/4–1/2, demonstrating CPC/CHA combination is desirable for rapid disinfection.…”

Section: Discussionmentioning

confidence: 99%

Combination of Cetylpyridinium Chloride and Chlorhexidine Acetate: A Promising Candidate for Rapid Killing of Gram-Positive/Gram-Negative Bacteria and Fungi

Zhu

Zhang

et al. 2023

Curr Microbiol

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Combined use of the present antimicrobial drugs has been proved to be an alternative approach for antimicrobial agents’ development since the co-existed of the drugs working in different mechanism have been demonstrated potentially enhance their antimicrobial activity. In this work, antibacterial and antifungal activity of the cetylpyridinium chloride (CPC)/chlorhexidine acetate (CHA) combination was evaluated for the first time, while a universal concentration for the rapid killing of gram-positive/gram-negative bacteria and fungi was also proposed. The minimum inhibitory concentrations (MIC) of CPC and CHA used alone or in combination were first measured, showing that the combined treatment decreased the MIC against tested gram-positive/gram-negative bacteria and fungi to 1/8–1/2. Growth curve assays demonstrated CPC and CHA had dynamic combined effects against the tested microorganisms at the concentration equal to MIC. Besides, combined use of these two drugs could also enhance their biocidal activity, which was illustrated by fluorescence microscopy and SEM images, as well as soluble protein measurement. More importantly, in vitro acute eye and skin irritation tests showed short-term contact with CPC/CHA combination would not cause any damage to mammalian mucosa and skin. In a word, CPC/CHA combination exhibited broad-spectrum antibacterial and antifungal activity against tested gram-positive/gram-negative bacteria and fungi while without any acute irritation to mammalian mucosa and skin, providing a new perspective on the selection of personal disinfectants. Supplementary Information The online version contains supplementary material available at 10.1007/s00284-023-03198-z.

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Section: Discussionmentioning

confidence: 99%

Combination of Cetylpyridinium Chloride and Chlorhexidine Acetate: A Promising Candidate for Rapid Killing of Gram-Positive/Gram-Negative Bacteria and Fungi

Zhu

Zhang

et al. 2023

Curr Microbiol

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“…VP2 protein synthesis was greatly reduced after inactivation by PAA, Virkon S and JM within 1 h, and the inoculation of F81 cells failed to produce the corresponding CPE. According to the results of the HA, the HA titer of FPV after disinfectant treatment would decrease partially, and we assumed this may because the disinfectant only inhibits partial VP2 protein synthesis [ 26 ]. However, taking the results of IFA together, an efficacious disinfectant could still inhibit virus proliferation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Inactivation Efficacy of Four Disinfectants for Feline Parvovirus Derived from Giant Panda

Peng

Zhang

et al. 2023

Microorganisms

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Feline panleukopenia (FPL) is a highly contagious acute infectious disease caused by feline parvovirus (FPV). FPV has also been found in giant pandas with clinical signs of vomiting and mild diarrhea, posing a threat to this vulnerable species. Cleaning and disinfection may be one of the most efficacious ways to prevent FPV spread in the habitat of giant pandas. This study evaluated the inactivation effect of peracetic acid (PAA), povidone-iodine (PVP-I), glutaral and deciquam solution (JM) and Virkon S. The tissue culture infective dose (TCID50) assay indicated that the virus may be totally inactivated by JM, PAA and Virkon S. Meanwhile, the hemagglutination (HA) assay showed a high inactivation efficiency of PAA and Virkon S. The analysis of Western blot revealed that PAA, Virkon S and JM can inhibit the structural protein synthesis. Taken together, our findings demonstrated that PAA could rapidly and efficiently inactivate FPV, representing an efficacious disinfectant for FPV control.

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“…Olanexidine gluconate-containing hand rub exhibited virucidal activity against murine norovirus and GI, GII, and GIV HuNoV strains when evaluated with culture-independent methods based on reverse transcription-quantitative PCR (RT-qPCR) and RNase or photoreactive intercalators to detect genome derived from intact viruses ( 16 ). Recently, OLG-HR has been shown to increase the aggregation of VLPs in a dose-dependent manner ( 28 ). However, its activity on human viruses in culture-based methods remained to be determined.…”

Section: Discussionmentioning

confidence: 99%