Disinfection and Antimicrobial Processes

Kuo, Jeff; Abustan, Laureen

doi:10.2175/106143009x12445568399695

Cited by 5 publications

(1 citation statement)

References 205 publications

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“…Typically the final step in wastewater treatment, disinfection of wastewater in the US is focused entirely on reducing numbers of fecal coliforms present in polished effluent to levels below the USEPA mandated criterion. Chlorination and UV-irradiation are the most commonplace because of the relative cost and equipment problems when using ozone as a disinfectant [16,17]. However, ozone is gaining renewed interest in wastewater treatment due to decreasing water supplies, ensuing stricter discharge limits, and increasing concern regarding pharmaceutical and personal care products along with other emerging contaminants [18].…”

Section: Introductionmentioning

confidence: 99%

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

Asfahl¹,

Savin²

2012

AiM

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Broad-host-range plasmids are frequently associated with antibiotic resistance genes and can quickly spread antibiotic resistant phenotypes among diverse bacterial populations. Wastewater treatment plants have been identified as reservoirs for broad-host-range plasmids carrying resistance genes. The threat of broad-host-range plasmids released into the environment from wastewater treatment plants has identified the need for disinfection protocols to target broad-hostrange plasmid destruction. Here we evaluate the efficacy of dissolved ozone at 2 and 8 mg·L -1 as a primary means for the destruction of broad-host-range plasmid and chromosomal DNA in simulated effluent. Pilot-scale tests using an experimental unit were carried out in municipal wastewater treatment plant effluent and compared with ultraviolet (UV)-irradiation and chlorination methodologies. Genes specific to Escherichia coli (uidA) and IncP broad-host-range plasmids (trfA) were monitored using real-time quantitative polymerase chain reaction (qPCR), and total DNA was monitored using absorbance spectroscopy. In wastewater treatment plant experiments, E. coli qPCR results were compared to a recognized culture-based method (Colilert ® ) for E. coli. In laboratory experiments, dissolved ozone at 8 mg·L -1 significantly destroyed 93% total, 98% E. coli, and 99% of broad-host-range plasmid DNA. Ozonation, UV-irradiation, and chlorination significantly reduced DNA concentrations and culturable E. coli in wastewater treatment plant effluent. Chlorination and UV disinfection resulted in 3-log decreases in culture-based E. coli concentrations in wastewater treatment plant effluent while changes were not significant when measured with qPCR. Only ozonation significantly decreased the IncP broad-host-range plasmid trfA gene, although concentrations of 2.2 × 10 5 copies trfA·L -1 remained in effluent. Disinfection processes utilizing high dissolved ozone concentrations for the destruction of emerging contaminants such as broad-host-range plasmid and total DNA may have utility as methods to ensure downstream environmental health and safe water reuse become more important.

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Section: Introductionmentioning

confidence: 99%

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

Asfahl¹,

Savin²

2012

AiM

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ChemInform Abstract: Disinfection and Antimicrobial Processes (for Water/Wastewater and Biosolids)

Kuo

Abustan

2010

ChemInform

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A review of research advances on disinfection strategies for biofilm control in drinking water distribution systems

Oliveira,

Gomes,

Simões

et al. 2024

Water Research

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Disinfection and Antimicrobial Processes

Cited by 5 publications

References 205 publications

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

ChemInform Abstract: Disinfection and Antimicrobial Processes (for Water/Wastewater and Biosolids)

A review of research advances on disinfection strategies for biofilm control in drinking water distribution systems

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Disinfection and Antimicrobial Processes

Cited by 5 publications

References 205 publications

Destruction of &lt;i&gt;Escherichia coli&lt;/i&gt; and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

Destruction of &lt;i&gt;Escherichia coli&lt;/i&gt; and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

ChemInform Abstract: Disinfection and Antimicrobial Processes (for Water/Wastewater and Biosolids)

A review of research advances on disinfection strategies for biofilm control in drinking water distribution systems

Contact Info

Product

Resources

About

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions