Disinfecting and Shaping Type I C-shaped Root Canals: A Correlative Micro–computed Tomographic and Molecular Microbiology Study

Gazzaneo, Isbelia; Amoroso-Silva, Pablo Andrés; Pacheco‐Yanes, Juan; Alves, Flávio R.F.; Marceliano-Alves, Marília Fagury Videira; Olivares, Patrícia; Meto, Aida; Mdala, Ibrahimu; Siqueira, José F.; Rôças, Isabela N.

doi:10.1016/j.joen.2020.11.007

Cited by 25 publications

(25 citation statements)

References 33 publications

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“…It was reported that the materials and the obturation technique considerably affected the voids and the total volume of obturation material [ 82 ]. The disinfecting and shaping characteristics of some preparation protocols in C-shaped root canals were evaluated by Gazzaneo et al [ 83 ] employing a correlative microcomputed tomographic and molecular microbiology work. The BioRaCe and XP-endo Shaper systems were reported to have similar disinfection and shaping properties in mandibular molars having C-shaped canals.…”

Section: Applications For Nanotechnology In Endodonticsmentioning

confidence: 99%

“…The BioRaCe and XP-endo Shaper systems were reported to have similar disinfection and shaping properties in mandibular molars having C-shaped canals. In addition, the supplementary steps with the Hedström file and the XP-endo Finisher promoted similar decreases in the unprepared canal surfaces, whereas the effects were not sufficient to have significant improvements in bacterial elimination; thus, the development of more effective strategies would be needed for the disinfection of the mentioned canals [ 83 ].…”

Section: Applications For Nanotechnology In Endodonticsmentioning

confidence: 99%

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Recent Progress on the Applications of Nanomaterials and Nano-Characterization Techniques in Endodontics: A Review

Özdemir

Kopaç

2022

Materials

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The impact of nano-based technologies in endodontics for the identification and treatment of various dental infections is showing fast progress. Studies show that nanoparticles could serve as useful agents with many beneficial results and continue to be promising in the field of endodontics. To ensure progress and improvements on novel nanomaterials in relation to their physicochemical and biological properties, nano-identification methods for the detection and evaluation of diseases need to be further highlighted. This study aims to review the current technological progress and recent research outcomes as well as possible prospective applications of nano-based technologies in endodontics. A comprehensive literature survey has been carried out on the utilizations of nanomaterials and nano-characterization techniques in endodontics. The current status and recent applications in endodontics are discussed with illustrative examples. The results have shown that the progress and improved accuracy of nano-identification techniques enabled a better characterization, evaluation and selection of appropriate treatment plans for endodontics-related diseases. The results have been inspiring for further clinical investigations. Nano-endodontics is still a developing field with a strong potential for revolutions of novel materials and techniques in the diagnosis and treatment of dental diseases. Further improvements in nanoparticles properties will pave the way for the development of many beneficial endodontic therapeutic agents. The future looks encouraging for sustainable products and testing methods for clinical endodontic applications.

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Section: Applications For Nanotechnology In Endodonticsmentioning

confidence: 99%

Section: Applications For Nanotechnology In Endodonticsmentioning

confidence: 99%

Recent Progress on the Applications of Nanomaterials and Nano-Characterization Techniques in Endodontics: A Review

Özdemir

Kopaç

2022

Materials

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“…This anatomical finishing file has an adaptive core technology, which presumably adapts to the root canal morphology to facilitate disinfection. Previous studies have demonstrated promising results using the XP‐EF to remove debris and the smear layer (Alakshar et al, 2020; De‐Deus, et al, 2019a; De‐Deus, , et al, 2019b), root canal fillings (De‐Deus, et al, 2019a; De‐Deus,et al, 2019b; Silva et al, 2018), calcium hydroxide (Keskin et al, 2017), antibiotic pastes (Turkaydin et al, 2017), biofilms (Bao et al, 2017), and disinfect bacteria (Amaral et al, 2020; Ballal et al, 2020; Carvalho et al, 2019; Gazzaneo et al, 2021). However, up to now, there has been no study evaluating XP‐EF's ability to improve LPS disinfection in root canal systems.…”

Section: Introductionmentioning

confidence: 99%

A modified dentine infection model with fluorescent lipopolysaccharide and lipopolysaccharides sampling technique to compare XP‐Endo finisher and passive ultrasonic irrigation

Alfirdous

Alquiria

Jacinto³

et al. 2022

Int Endodontic J

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Aim:The lipopolysaccharides-dentine-infection (LPS-dentine-infection) models and sampling techniques frequently used to evaluate LPS disinfection have limitations.In this study, a LPS-dentine-infection model was devised using fluorescent conjugate LPS. Secondly, a sampling technique using cryogenic grinding for intraradicular LPS analysis was evaluated. Thirdly, the effectiveness of the XP-endo Finisher (XP-EF) was compared with passive ultrasonic irrigation (PUI) in removing LPS from root canal system.Methodology: Sixty-nine mandibular premolars were submitted to dentine pretreatment and inoculated with fluorescent LPS conjugate (Alexa Fluor® 594). Twentythree teeth were analysed under confocal laser scanning microscopy (CLSM) to validate this modified LPS-dentine-infection model. Forty-six teeth were randomly divided into two experimental groups: XP-EF (n = 23) and PUI (n = 23). All teeth were instrumented with XP-endo shaper (XPS; FKG Dentaire) and 2.5% NaOCl. The root canals were sampled with paper points before (s1) and after (s2) instrumentation and after supplemental treatment (s3) with XP-EF and PUI. After s3, all roots were cryogenically ground for intraradicular LPS analysis (s4). Limulus amebocyte lysate assay was used for LPS quantification. The Friedman test was used for differences in LPS among four time-points (s1, s2, s3, and s4). Dunn's test was used for pairwise testing of time-points. The significance level was set at 5% (p < .05).Results: Fluorescent LPS conjugate was detected in 100% of the samples under CLSM with a penetration depth of approximately 400 μm into dentine. Chemo-mechanical preparation using XPS files significantly reduced LPS levels (p < .05). Both the XPS and PUI improved the LPS disinfection (p < .05), with no difference between them (p > .05). LPS was recovered from all samples after cryogenic grinding. The residual amount of LPS detected using the cryogenically sampling technique at s4 was approximately three times greater than with the paper-point sampling technique at s3. Conclusion:This study established a modified LPS-dentine-infection model using fluorescent conjugate LPS, and validated a LPS sampling technique for using How to cite this article:

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“…In most of the cases, periapical inflammatory processes penetrate inside the tooth without symptoms [ 8 ]. In traditional endodontics for canal-disinfection, active irrigants are necessary to penetrate the endodontic cavity and peripheral parts of root canal system; their effects are reduced when encountering pulp and dentin components [ 9 , 10 , 11 ]. Nowadays, several data have demonstrated that different components in dentin may affect the antibacterial properties of compounds such as sodium hypochlorite (NaOCl), chlorhexidine digluconate (CHX), iodine potassium iodide (IPI), and Tetraclean [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Copper–Calcium Hydroxide and Permanent Electrophoretic Current for Treatment of Apical Periodontitis

et al. 2021

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Endodontic failure has been and continues to be a problem for endodontics-specialists. Complicated anatomy, numerous foramens, and accessory canals are an environment for microorganisms to infect the teeth. The purpose of the present work was to evaluate the regeneration of copper–calcium hydroxide (Cupral)-endodontically treated teeth diagnosed with apical periodontitis using an electrophoresis technique. In total, 132 patients, aging from 19 to 65 years old, underwent endodontic treatment mono- and multi-radicular teeth, with complicated canals from January 2019 to June 2020. The patients were divided into two groups: (i) the control group—which included 54 patients (n = 62 teeth) receiving endodontic paste (Calcipast + 1) and, as final filling, the AH-PlusTM cement—and (ii) the Cupral group, which included 78 patients (n = 80 teeth) receiving Cupral paste plus the electrophoretic current and, as final filling, the Atacamit-alkaline cement. The clinical cases were periodically observed along an 18-month follow-up period via radiography. Data were expressed as focal size of the lesions (mean ± standard error (SEM) of all the radiographic outcomes) observed in each group at each interval point. Statistical analysis was performed using the Student’s t-test that allowed us to compare the control and Cupral groups; the statistical significance was set at p < 0.05 and p < 0.01, where the latter was highly significant. Before treatments, the focal sizes were 4.8 mm and 4.95 mm for control and Cupral-treated groups, respectively. After 6 months, the mean focal sizes were 3.9 mm and 2.14 mm for the control and Cupral groups, respectively. After 12 months, in the control group, the mean focal size was measured at 2.8 mm, while, in Cupral group, the lesion size decreased down to 0.31 mm and a highly dynamic regeneration of the destructive focal-bone occurred. After 18 months, the lesions were further significantly reduced in the control group (mean values of 2.62 mm), while they were barely detectable in the Cupral group (0.2 mm). In conclusion, we provide initial evidence that the Cupral-electrophoresis methodology is effective in treating destructive periodontitis of teeth with problematic canals up to 18 months, thus allowing teeth preservation.

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Disinfecting and Shaping Type I C-shaped Root Canals: A Correlative Micro–computed Tomographic and Molecular Microbiology Study

Cited by 25 publications

References 33 publications

Recent Progress on the Applications of Nanomaterials and Nano-Characterization Techniques in Endodontics: A Review

Recent Progress on the Applications of Nanomaterials and Nano-Characterization Techniques in Endodontics: A Review

A modified dentine infection model with fluorescent lipopolysaccharide and lipopolysaccharides sampling technique to compare XP‐Endo finisher and passive ultrasonic irrigation

Copper–Calcium Hydroxide and Permanent Electrophoretic Current for Treatment of Apical Periodontitis

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