Disialoganglioside (GD3) Synthase Gene Expression Suppresses Vascular Smooth Muscle Cell Responses via the Inhibition of ERK1/2 Phosphorylation, Cell Cycle Progression, and Matrix Metalloproteinase-9 Expression

Moon, Sung‐Kwon; Kim, Hong-Man; Lee, Young Choon; Kim, Cheorl-Ho

doi:10.1074/jbc.m313462200

Cited by 69 publications

(81 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, up-regulation of MMP expression has been reported to be dependent on activation of the raf/MEK/ERK signaling pathway, which strictly regulates gene expression (31)(32)(33). Consistently, whereas the high expression of MMP-2 and MMP-9 in C-26 cells correlates with constitutive activation of ERK1/2 protein kinases (Fig.…”

Section: Discussionsupporting

confidence: 48%

Hyperforin Inhibits Cancer Invasion and Metastasis

Donà

Dell’Aica²,

Pezzato³

et al. 2004

Cancer Research

126

105

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 48%

Hyperforin Inhibits Cancer Invasion and Metastasis

Donà

Dell’Aica²,

Pezzato³

et al. 2004

Cancer Research

126

105

View full text Add to dashboard Cite

“…It has recently been shown that ganglioside GM3 inhibited MMP-9 expression induced by fibronectin or epidermal growth factor (EGF) in SCC12 carcinoma cells (21). In addition, the present results are in agreement with a previous report showing the inhibition of MMP-9 expression in GD3 synthase gene transfectants (33). However, the differences in the effects of the molecular action mechanisms of the GM3 and the GD3 synthase genes remain to be elucidated.…”

Section: Discussionsupporting

confidence: 82%

“…Studies have shown that GM1 and GM2 induce VSMC proliferation through ERK1/2 activation (31). Several studies have shown a dual function of ganglioside GD3, in both cell proliferation and inhibition of cell growth in VSMC (32,33). A number of studies have demonstrated massive and excessive accumulation of GM3 in atherosclerotic lesions (23,24).…”

Section: Discussionmentioning

confidence: 99%

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Moon¹

2010

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Abstract. The natural accumulation of ganglioside GM3 (N-glycolylneuraminic acid) on atherosclerotic lesions is a common theory. The present study is the first to examine the effects of the GM3 synthase gene on the responses of vascular smooth muscle cells (VSMC) to tumor necrosis factor-· (TNF-·). We found that overexpression of the GM3 synthase gene inhibited DNA synthesis and ERK1/2 activity induced by TNF-· in VSMC, whereas the basal levels of DNA synthesis and ERK1/2 activity remained unchanged. In addition, GM3 synthase gene transfectants significantly reduced the migration and invasion of VSMC following TNF-· treatment, compared with empty vector transfectants. Furthermore, TNF-·-induced matrix metalloproteinase-9 (MMP-9) expression and promoter activity were also decreased in GM3 synthase gene transfectants. GM3 synthase gene expression markedly suppressed the TNF-·-stimulated transcriptional activity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB), which are the controlling factors of MMP-9 expression. Consistent with these results, the addition of anti-GM3 antibody into the GM3 synthase gene transfectants blocked inhibition of DNA synthesis, ERK1/2 activity, migration and invasion. Finally, GM3 synthase gene transfectants treated with anti-GM3 antibody reversed the suppression of MMP-9 expression by reducing AP-1 and NF-κB binding activity. These results suggest regulatory roles for the GM3 synthase gene in VSMC proliferation and migration during the formation of atherosclerotic lesions.

show abstract

“…Numerous studies have demonstrated that JNK1/2 and ERK1/2 transcriptionally regulate the expression of MMP-2 and MMP-9, which results in regulation of cell migration and invasion (25)(26)(27). In human bladder cancer cells, upregulation of the MAPK pathways may result in migration and regulation of the expression levels of MMPs (28).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of resveratrol on the adhesion, migration and invasion of human bladder cancer cells

Bai

Yang

Zhang

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract.Resveratrol is a polyphenolic compound, which has been revealed to induce apoptosis in numerous human cancer cells; however, the effects of resveratrol on the migration and invasion of human bladder cancer cells have not been reported. The present study aimed to evaluate the anti-metastatic potential of resveratrol against bladder cancer and its mechanism of action. The results indicated that resveratrol inhibits the adhesion, migration and invasion of bladder cancer cells in a dose-dependent manner. Resveratrol was shown to significantly inhibit the expression and secretion of matrix metalloproteinase (MMP)-2 and MMP-9 in bladder cancer cells. Furthermore, resveratrol suppressed the phosphorylation of c-Jun N-terminal kinase and extracellular signal-regulated protein kinase. In conclusion, the present study is the first, to the best of our knowledge, to demonstrate that resveratrol may be considered a novel anticancer agent for the treatment of bladder cancer via the inhibition of migration and invasion. IntroductionBladder cancer is the fourth most common type of cancer, and was the cause of >11,510 cases of cancer-associated mortality among men in the USA in 2014 (1). The majority of bladder cancer cases are noninvasive, low-grade transitional cell carcinoma (TCC); however, ≤30% of all diagnosed tumors are classified as invasive TCC, which is associated with high mortality (2). Notably, among all of the various types of cancer, bladder cancer exhibits a significantly high incidence of recurrence post-treatment, and therefore is recognized as the most expensive cancer to treat. Due to the high prevalence and frequent tumor recurrence associated with bladder cancer, the exploration of preventative strategies is required.Previous epidemiological and scientific studies have indicated that plant-derived phytochemicals may be beneficial in the prevention and treatment of cancer (3-5). Resveratrol is a polyphenolic compound, which is present in grapes (red wine), knotweed, peanuts, mulberries and other plants. Numerous studies have suggested that resveratrol may prevent the progression of various pathologies, including vascular diseases, ischemic injuries, cancer and neurodegenerative disorders (6-8). Furthermore, it has previously been confirmed that resveratrol exerts antiproliferative and/or apoptotic effects on leukemia, prostate, breast and colon cancers cells in vitro (9-12). Previous studies regarding the anticancer effects of resveratrol have focused on its anti-invasive and anti-metastatic activities (13-15). However, to the best of our knowledge, there is currently no evidence as to whether resveratrol may inhibit the migration and invasion of human bladder cancer cells.Metastasis is one of the hallmarks of advanced cancer progression, and cancer cell migration and invasion are crucial events in bladder cancer metastasis. Matrix metalloproteinases (MMPs), including MMP-2 and MMP-9, degrade the basement membrane and extracellular matrix (ECM), and are therefore considered crucial proteolytic protei...

show abstract

Disialoganglioside (GD3) Synthase Gene Expression Suppresses Vascular Smooth Muscle Cell Responses via the Inhibition of ERK1/2 Phosphorylation, Cell Cycle Progression, and Matrix Metalloproteinase-9 Expression

Cited by 69 publications

References 48 publications

Hyperforin Inhibits Cancer Invasion and Metastasis

Hyperforin Inhibits Cancer Invasion and Metastasis

Suppression of vascular smooth muscle cell responses induced by TNF-α in GM3 synthase gene transfected cells

Inhibitory effects of resveratrol on the adhesion, migration and invasion of human bladder cancer cells

Contact Info

Product

Resources

About