Disentangling the evolutionary history of peri-Mediterranean cyprinids using host-specific gill monogeneans

Benovics, Michal; Vukić, Jasna; Šanda, Radek; Rahmouni, Imane; Šimková, Andrea Vetešníková

doi:10.1016/j.ijpara.2020.05.007

Cited by 16 publications

(19 citation statements)

References 79 publications

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“…5.8S , respectively [ 43 , 44 ]. Amplification reactions followed protocols optimized in Benovics et al [ 15 ]. The partial 28S region was amplified using the forward primer C1 (5′-ACCCGCTGAATTTAAGCA-3′) and reverse primer D2 (5′- TGGTCCGTGTTTCAAGAC-3′) [ 45 ], following the PCR protocol optimized by Šimková et al [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…The Dactylogyrus species were divided into four categories: (1) strict specialists parasitizing single cyprinoid species, (2) intermediate specialists parasitizing congeneric host species, (3) transitional generalists parasitizing cyprinoid species belonging to a single subfamily, and (4) common generalists parasitizing species belonging to a single cyprinoid family, i.e., in this case, to Cyprinidae. The level of host specificity for each investigated Dactylogyrus species was determined from the compilation of data from various sources, i.e., checklists ([ 9 , 54 – 57 ] and references within), the determination key including original descriptions and/or host reports included in Pugachev et al [ 11 ], and other studies reporting the presence of the investigated species [ 8 , 15 , 30 – 32 , 35 , 58 – 69 ]. All published reports were thoroughly evaluated, and potential misidentifications were not included.…”

Section: Methodsmentioning

confidence: 99%

“…Some generalist species (e.g., D. sphyrna, D. vastator , and D. vistulae ) have not developed a haptoral ventral connective bar. As this morphological feature is present in Dactylogyrus species of different phylogenetic lineages, it was hypothesized by Benovics et al [ 15 ] that the secondary loss of the ventral connective bar may allow the parasite to infect a wider range of hosts. Concurrently, Šimková et al [ 16 ] hypothesized that attachment organs with large-sized anchoral sclerites promote the colonization of several phylogenetically distant host species.…”

Section: Introductionmentioning

confidence: 99%

“…According to recent studies by Šimková et al [ 29 ] and Benovics et al [ 15 , 30 ], endemic cyprinids of the peri-Mediterranean are parasitized by Dactylogyrus species belonging to at least three divergent phylogenetic lineages. The individual lineages are of uncertain origin and all aforementioned studies hypothesized their ancestry, which is most likely interconnected with the phylogeography and historical dispersion of their cyprinoid hosts.…”

Section: Introductionmentioning

confidence: 99%

“…The individual lineages are of uncertain origin and all aforementioned studies hypothesized their ancestry, which is most likely interconnected with the phylogeography and historical dispersion of their cyprinoid hosts. Reconstructions of Dactylogyrus phylogenies are commonly based on multiple molecular markers; such as partial genes coding ribosomal subunits ( 18S, 28S, 5.8S ), and the internal transcribed spacer 1 ( ITS1 ) [ 15 , 16 , 29 – 35 ]. The ribosomal subunits have been used to resolve phylogenetic relationships between divergent platyhelminth taxa for quite a long time (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and morphological phylogeny of host-specific Dactylogyrus parasites (Monogenea) sheds new light on the puzzling Middle Eastern origin of European and African lineages

et al. 2021

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Background Freshwater fauna of the Middle East encompass elements shared with three continents—Africa, Asia, and Europe—and the Middle East is, therefore, considered a historical geographic crossroad between these three regions. Even though various dispersion scenarios have been proposed to explain the current distribution of cyprinids in the peri-Mediterranean, all of them congruently suggest an Asian origin for this group. Herein, we investigated the proposed scenarios using monogenean parasites of the genus Dactylogyrus, which is host-specific to cyprinoid fishes. Methods A total of 48 Dactylogyrus species parasitizing cyprinids belonging to seven genera were used for molecular phylogenetic reconstruction. Taxonomically important morphological features, i.e., sclerotized elements of the attachment organ, were further evaluated to resolve ambiguous relationships between individual phylogenetic lineages. For 37 species, sequences of partial genes coding 18S and 28S rRNA, and the ITS1 region were retrieved from GenBank. Ten Dactylogyrus species collected from Middle Eastern cyprinoids and D. falciformis were de novo sequenced for the aforementioned molecular markers. Results The phylogenetic reconstruction divided all investigated Dactylogyrus species into four phylogenetic clades. The first one encompassed species with the “varicorhini” type of haptoral ventral bar with a putative origin linked to the historical dispersion of cyprinids via the North African coastline. The second clade included the majority of the investigated species parasitizing various phylogenetically divergent cyprinid hosts. The morphological and molecular data suggest the ancestral diversification of the species of this clade into two groups: (1) the group possessing the haptoral ventral bar of the “cornu” type, and (2) the group possessing the “wunderi” type. Dactylogyrus diversification apparently occurred in the Middle East, which is indicated by the presence of species with all morphotypes in the region. The last two clades included species parasitizing cyprinids with an East Asian origin, and species possessing the “magnihamatus” type of ventral bar. Conclusions The molecular data suggest that some morphological characters of host-specific parasites may undergo convergent evolution in the divergent lineages, and therefore, to fully resolve the phylogenetic relationships among host-specific parasites, an integrative approach combining morphological and molecular data is still needed. In addition, our study indicates that parasite diversity in many regions is still under-explored, and thus we highlight the importance of studies of host-associated parasites, especially in the context of freshwater fish biogeography. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular and morphological phylogeny of host-specific Dactylogyrus parasites (Monogenea) sheds new light on the puzzling Middle Eastern origin of European and African lineages

et al. 2021

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Multiple introduction pathways of non-native Phoxinus minnows (Teleostei: Leuciscidae) in Corsica revealed by its hidden diversity and their parasites

Esposito,

Denys,

Haÿ

et al. 2024

Biol Invasions

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The introduction of freshwater fish species is a leading cause of aquatic biodiversity erosion and can spread parasites to native populations. Hidden diversity evidenced by recent taxonomic revisions can add further complexity to the issue by rendering biological assessment data incomplete. The Eurasian minnows Phoxinus are one such example of cryptic diversity, with several described species being invasive. Current non-native fish populations in the small Mediterranean island of Corsica (France) are the result of successive waves of introductions, including several Phoxinus species. This study aims at determining which Phoxinus species were introduced to Corsica using the cytochrome oxidase subunit I barcoding marker, reconstructing their introduction routes and examining their parasite communities. The study found four species in Corsica: Phoxinus phoxinus and Phoxinus csikii mainly in the northernmost studied drainage basin and Phoxinus dragarum and Phoxinus septimaniae in the Tavignano drainage basin. P. phoxinus and P. csikii were most likely introduced through a live bait wholesaler while P. dragarum and P. septimaniae were probably introduced by recreational anglers bringing their bait from continental France. The molecular study of their Gyrodactylus (Platyhelminthes: Monogenea) parasites with the ITS marker allowed us to hypothesize inter-drainage basin secondary introduction routes for P. phoxinus and P. dragarum. In several sampling sites, Phoxinus minnows had black spot disease caused by encysted metacercariae of Digenea, likely Posthodiplostomum cuticola. These parasites were also found on the brown trout Salmo trutta in a locality where this patrimonial species co-occurs with Phoxinus minnows. Barcoding should be used in fish communities monitoring to help to accurately identify cryptic species.

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Mitochondrial genetic diversity, phylogeography and historical demography of Moroccan native freshwater fishes: a case study of the genus Luciobarbus Heckel, 1843

et al. 2022

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Disentangling the evolutionary history of peri-Mediterranean cyprinids using host-specific gill monogeneans

Cited by 16 publications

References 79 publications

Molecular and morphological phylogeny of host-specific Dactylogyrus parasites (Monogenea) sheds new light on the puzzling Middle Eastern origin of European and African lineages

Molecular and morphological phylogeny of host-specific Dactylogyrus parasites (Monogenea) sheds new light on the puzzling Middle Eastern origin of European and African lineages

Multiple introduction pathways of non-native Phoxinus minnows (Teleostei: Leuciscidae) in Corsica revealed by its hidden diversity and their parasites

Mitochondrial genetic diversity, phylogeography and historical demography of Moroccan native freshwater fishes: a case study of the genus Luciobarbus Heckel, 1843

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