Disentangling the Effects of Ocean Carbonation and Acidification on Elemental Contents and Macromolecules of the Coccolithophore Emiliania huxleyi

Xie, Emei; Xu, Kui; Li, Zhengke; Li, Wei; Yi, Xiangqi; Li, Hongzhou; Han, Yong-He; Zhang, Hong; Zhang, Yong

doi:10.3389/fmicb.2021.737454

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…Elemental variation in phytoplankton in response to climate change could modify their nutritional quality, and alter biogeochemistry (Hutchins et al, 2009). Understanding how elements vary in macromolecular components (e.g., carbohydrates, lipids, and proteins), depending on climate change conditions, could improve our knowledge of coccolithophore contribution to the marine carbon and nitrogen cycles (Xie et al, 2021). Coccolithophores have been reported to adapt to long‐term climate change conditions by modifying their nutrient accumulation, carbon production, and the C:N ratio (Jin et al, 2013; Lohbeck et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Transitional traits determine the acclimation characteristics of the coccolithophore Chrysotila dentata to ocean warming and acidification

Thangaraj

Liu

Guo

et al. 2023

Environmental Microbiology

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Ocean warming and acidification interactively affect the coccolithophore physiology and drives major biogeochemical changes. While numerous studies investigated coccolithophore under short-term conditions, knowledge on how different transitional periods over long-exposure could influence the element, macromolecular and metabolic changes for its acclimation are largely unknown. We cultured the coccolithophore Chrysotila dentata, (culture generations of 1st, 10th, and 20th) under present (lowtemperature low-carbon-dioxide [LTLC]) and projected (high-temperature high-carbon-dioxide [HTHC]) ocean conditions. We examined elemental and macromolecular component changes and sequenced a transcriptome. We found that with long-exposure, most physiological responses in HTHC cells decreased when compared with those in LTLC, however, HTHC cell physiology showed constant elevation between each generation. Specifically, compared to 1st generation, the 20th generation HTHC cells showed increases in quota carbon (Qc:29%), nitrogen (Q N :101%), and subsequent changes in C:N-ratio (68%). We observed higher lipid accumulation than carbohydrates within HTHC cells under long-exposure, suggesting that lipids were used as an alternative energy source for cellular acclimation. Protein biosynthesis pathways increased their efficiency during long-term HTHC condition, indicating that cells produced more proteins than required to initiate acclimation. Our findings suggest that the coccolithophore resilience increased between the 1st-10th generation to initiate the acclimation process under ocean warming and acidifying conditions.

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Section: Introductionmentioning

confidence: 99%

Transitional traits determine the acclimation characteristics of the coccolithophore Chrysotila dentata to ocean warming and acidification

Thangaraj

Liu

Guo

et al. 2023

Environmental Microbiology

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“…Diverse metabolic responses of the coccolithophore E. huxleyi to ocean acidification and to combinations of ocean acidification with other environmental factors have been described (Tong et al 2017;Gafar et al 2019). Xie et al (2021) studied the effects of high and low DIC concentration (from 900 to 4,930 μmolkg -1 ) and reduced pH value (from 8.04 to 7.70) on physiological rhythms, element contents and macromolecules of the coccolithophore Emiliania huxleyi, concluding that its response is highly dependent on the DIC. Compared to high pH conditions, low pH and DIC concentration led to increases in particulate organic carbon (POC)…”

Section: Total Carbohydrate Content Of Cellsmentioning

confidence: 99%

Variations of polyphenols and carbohydrates of Emiliania huxleyi grown under simulated ocean acidification conditions

Rico,

Santiago-Díaz,

Samperio-Ramos

et al. 2024

Preprint

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Abstract. Global environmental changes strongly affect the growth and biochemical composition of microalgae. Cultures of the coccolithophore Emiliania huxleyi were grown under four different CO2-controlled pH conditions (7.75, 7.90, 8.10, and 8.25) to improve understanding of the adaptive mechanisms of these organisms through changes in phenolic compounds and carbohydrate content and composition under ocean acidification (OA) scenarios. The highest algal biomass peaks, 1.07 (± 0.10) and 1.04 (± 0.06) × 108 cells L−1, were observed in the microcosms with intermediate CO2 levels (pH 8.10 and 7.90 respectively). Intra- and extracellular phenolic compounds were identified and quantified by Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). The highest concentrations of total exuded phenolics were found in cultures with lower cell densities, at pH 8.25 (43±3 nM) and 7.75 (18.0±0.9 nM). Accumulation of intracellular phenolic compounds was observed in cells with decreasing pH, reaching the maximum level (9.24±0.19 attomole cell-1) at the lowest pH (7.75). The total carbohydrate content inside the cells increased with decreasing pH from 8.25 to 8.10, remaining constant at pH 7.90, and decreasing at lower pH. The presence of antioxidants was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition and ferric-reducing antioxidant power (FRAP) assays. The highest activity in both tests was exhibited by cells grown at pH 7.75.

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“…Coccolithophore blooms in the field are generally observed in environments with a high irradiance [ 21 ]. Previous studies have mainly focused on the sensitivity of the cosmopolitan species Emiliania huxleyi and Gephyrocapsa oceanica to environmental changes [ 13 , 22 , 23 ]. Nonetheless, marine coccolithophores have a global distribution, with a diversity of ~280 morphospecies [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Ocean Acidification Affects the Response of the Coastal Coccolithophore Pleurochrysis carterae to Irradiance

Wu,

Guo,

Duan

et al. 2023

Biology

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The ecologically important marine phytoplankton group coccolithophores have a global distribution. The impacts of ocean acidification on the cosmopolitan species Emiliania huxleyi have received much attention and have been intensively studied. However, the species-specific responses of coccolithophores and how these responses will be regulated by other environmental drivers are still largely unknown. To examine the interactive effects of irradiance and ocean acidification on the physiology of the coastal coccolithophore species Pleurochrysis carterae, we carried out a semi-continuous incubation experiment under a range of irradiances (50, 200, 500, 800 μmol photons m−2 s−1) at two CO2 concentration conditions of 400 and 800 ppm. The results suggest that the saturation irradiance for the growth rate was higher at an elevated CO2 concentration. Ocean acidification weakened the particulate organic carbon (POC) production of Pleurochrysis carterae and the inhibition rate was decreased with increasing irradiance, indicating that ocean acidification may affect the tolerating capacity of photosynthesis to higher irradiance. Our results further provide new insight into the species-specific responses of coccolithophores to the projected ocean acidification under different irradiance scenarios in the changing marine environment.

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Disentangling the Effects of Ocean Carbonation and Acidification on Elemental Contents and Macromolecules of the Coccolithophore Emiliania huxleyi

Cited by 4 publications

References 53 publications

Transitional traits determine the acclimation characteristics of the coccolithophore Chrysotila dentata to ocean warming and acidification

Transitional traits determine the acclimation characteristics of the coccolithophore Chrysotila dentata to ocean warming and acidification

Variations of polyphenols and carbohydrates of Emiliania huxleyi grown under simulated ocean acidification conditions

Ocean Acidification Affects the Response of the Coastal Coccolithophore Pleurochrysis carterae to Irradiance

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