Diseases of Lily

Chastagner, Gary A.; Tuyl, J.M. van; Verbeek, M.; Miller, William B.; Westerdahl, B. B.

doi:10.1007/978-3-319-39670-5_45

Cited by 7 publications

(17 citation statements)

References 170 publications

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“…In order to keep the genetic characteristics of lily flowers, lilies are usually clonally propagated using bulbs and scaling, which involves detaching scales from the bulb and planting individual scales to make new bulbs [ 4 ]. Due to the clonal propagation of lilies, most lily cultivars are frequently infected by diverse pathogenic microorganisms [ 5 ]. Of them, viruses infecting lily species cause serious damage to the quality and quantity of lily production.…”

Section: Introductionmentioning

confidence: 99%

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Cho

2018

BMC Genomics

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BackgroundThe lily is a perennial flowering plant belonging to the genus Lilium in the family Liliaceae. Most cultivated lily plants are propagated by bulbs. Therefore, numerous lily bulbs are frequently infected by diverse viruses causing viral diseases. To date, no study has examined the viromes of plants of one type with identical genetic backgrounds collected from different geographical regions.ResultsHere, we examined different viromes of the lily cultivar “Sorbonne” using 172 gigabytes of transcriptome data composed of 23 libraries from four different projects for the cultivar “Sorbonne.” We identified 396 virus-associated contigs from all but one library. We identified six different viruses, including Plantago asiatica mosaic virus (PlAMV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Tulip virus X (TVX), Lily mottle virus (LMoV), and Tobacco rattle virus (TRV). Of them, PlAMV was the most common virus infecting the lily. Scale and flower samples possessed a high number of virus-associated reads. We assembled 32 nearly complete genomes for the six identified viruses possessing the polyadenylate tails. Genomes of all six viruses were highly conserved in the lily cultivar “Sorbonne” based on mutation analysis. We identified defective RNAs from LSV, TVX, and PlAMV localized in the triple gene block region. Phylogenetic analyses showed that virus genomes are highly correlated with geographical regions and host plants.ConclusionsWe conducted comprehensive virome analyses of a single lily cultivar, “Sorbonne,” using transcriptome data. Our results shed light on an array of lily virome-associated topics, including virus identification, the dominant virus, virus accumulation in different plant tissues, virus genome assembly, virus mutation, identification of defective RNAs, and phylogenetic relationships of identified viruses. Taken together, we provide very useful methods and valuable results that can be applied in other virome-associated studies.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5138-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Cho

2018

BMC Genomics

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“…As with other potexviruses, PlAMV is readily transmitted by mechanical inoculation with sap extracts (Conijn, 2014; De Kock, 2013), but also spreads rapidly between infected and previously healthy lilies planted in a common container by uptake and probably exudation through the roots, and is remarkably stable in contaminated planting media (Conijn, 2014; De Kock, 2013). PlAMV is also transmitted between lilies during bulb washing and packing, which may be the major route of infection in commercial lilies (Chastagner et al, 2017; De Kock, Kok, et al, 2013; De Kock, Slootweg, et al, 2013). Fields in which PlAMV‐infected lilies were previously grown can retain viable virus, able to infect up to 8% of lily stocks previously thought to be free from PlAMV (De Kock, Slootweg, et al, 2013).…”

Section: Host Range Transmission and Symptomsmentioning

confidence: 99%

Plantago asiatica mosaic virus: An emerging plant virus causing necrosis in lilies and a new model RNA virus for molecular research

Komatsu

2022

Molecular Plant Pathology

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Taxonomy: Plantago asiatica mosaic virus belongs to the genus Potexvirus in the family Alphaflexiviridae of the order Tymovirales. Virion and genome properties: Plantago asiatica mosaic virus (PlAMV) has flexuous virions of approximately 490-530 nm in length and 10-15 nm in width. The genome of PlAMV consists of a single-stranded, positive-sense RNA of approximately 6.13 kb. It contains five open reading frames (ORFs 1-5), encoding a putative viral polymerase (RdRp), movement proteins (triple gene block proteins, TGBp1-3), and coat protein (CP), respectively. Host range: PlAMV has an exceptionally wide host range and has been isolated from various wild plants, including Plantago asiatica, Nandina domestica, Rehmannia glutinosa, and other weed plants. Experimentally PlAMV can infect many plant species including Nicotiana benthamiana and Arabidopsis thaliana. It also infects ornamental lilies and frequently causes severe necrotic symptoms. However, host range varies depending on isolates, which show significant biological diversity within the species. Genome diversity: PlAMV can be separated into five clades based on phylogenetic analyses; nucleotide identities are significantly low between isolates in the different clades. Transmission: PlAMV is not reported to be transmitted by biological vectors. Virions of PlAMV are quite stable and it can be transmitted efficiently by mechanical contact. Disease symptoms: PlAMV causes red-rusted systemic necrosis in ornamental lilies, but it shows much weaker, if any, symptoms in wild plants such as P. asiatica. Control: Control of the disease caused by PlAMV is based mainly on rapid diagnosis and elimination of the infected bulbs or plants.

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“…Genes encoding resistance to abiotic stresses such as cold, drought and salt were isolated from L. lancifolium and L. regale [78, 79]. Diseases induced by fungi such as Fusarium wilt and leaf blight [80, 81], and viruses such as cucumber mosaic virus (CMV), lily mottle virus (LMoV), lily symptomless virus (LSV) and tulip breaking virus (TBV) [80] cause severe damage to the lily industry. Genes encoding resistance to fungal and viral diseases have been found, including those resistant to Fusarium wilt in Asiatic hybrids [81] and L. regale [82–85], leaf blight in L. regal [86, 87], and TBV and LMoV in Longiflorum [72, 88, 89], Asiatic hybrids [73] and L. regal [74].…”

Section: Future Prospects and Conclusionmentioning

confidence: 99%

Section: Future Prospects and Conclusionmentioning

confidence: 99%

“…Virus diseases cause great economic losses in Lilium production [80]. Lilium elite cultivars used for flower production are vegetatively propagated and are therefore prone to virus infection [80]. Cryotherapy, a novel biotechnology for pathogen eradication [39], has been applied to 21 plant species and has successfully eradicated 28 viruses belonging to 18 genera of 8 families and one unassigned family [90].…”

Section: Future Prospects and Conclusionmentioning

confidence: 99%

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Development, progress and future prospects in cryobiotechnology of Lilium spp.

Zhang

et al. 2019

Plant Methods

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Lilium is one of the most popular flower crops worldwide, and some species are also used as vegetables and medicines. The availability of and easy access to diverse Lilium genetic resources are essential for plant genetic improvements. Cryopreservation is currently considered as an ideal means for the long-term preservation of plant germplasm. Over the last two decades, great efforts have been exerted in studies of Lilium cryopreservation and progress has been made in the successful cryopreservation of pollen, seeds and shoot tips in Lilium. Genes that exist in Lilium, including those that regulate flower shape, color and size, and that are resistant to cold stress and diseases caused by fungi and viruses, provide a rich source of valuable genetic resources for breeding programs to create novel cultivars required by the global floriculture and ornamental markets. Successful cryopreservation of Lilium spp. is a way to preserve these valuable genes. The present study provides updated and comprehensive information about the development of techniques that have advanced Lilium cryopreservation. Further ideas are proposed to better direct future studies on Lilium cryobiotechnology.

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Diseases of Lily

Cited by 7 publications

References 170 publications

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Plantago asiatica mosaic virus: An emerging plant virus causing necrosis in lilies and a new model RNA virus for molecular research

Development, progress and future prospects in cryobiotechnology of Lilium spp.

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