Disease progression-associated alterations in fecal metabolites in SAMP1/YitFc mice, a Crohn’s disease model

Komatsu, Yosuke; Shimizu, Yu; Yamano, Megumi; Kikuchi, Mani; Nakamura, Kiminori; Ayabe, Tokiyoshi; Aizawa, Tomoyasu

doi:10.1007/s11306-020-01671-5

Cited by 11 publications

(8 citation statements)

References 38 publications

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“…SCFAs is a kind of beneficial metabolite and derived from microbiota-accessible carbohydrates[ 128 ]. After microbial fermentation, acetate, propionate and butyrate are produced, also other gases such as gases methane and hydrogen sulfide are included[ 129 ].…”

Section: Tryptophan Metabolismmentioning

confidence: 99%

Role of metabolites derived from gut microbiota in inflammatory bowel disease

Wen¹,

Duan²

2022

WJCC

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Over the past two decades, it is improved gut microbiota plays an important role in the health and disease pathogenesis. Metabolites, small molecules produced as intermediate or end products of microbial metabolism, is considered as one of the major interaction way for gut microbiota with the host. Bacterial metabolisms of dietary substrates, modification of host molecules or bacteria are the major source of metabolites. Signals from microbial metabolites affect immune maturation and homeostasis, host energy metabolism as well as mucosal integrity maintenance. Based on many researches, the composition and function of the microbiota can be changed, which is also seen in the metabolite profiles of patients with inflammatory bowel disease (IBD). Additionally, some specific classes of metabolites also can trigger IBD. In this paper, definition of the key classes of microbial-derived metabolites which are changed in IBD, description of the pathophysiological basis of association and identification of the precision therapeutic modulation in the future are the major contents.

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Section: Tryptophan Metabolismmentioning

confidence: 99%

Role of metabolites derived from gut microbiota in inflammatory bowel disease

Wen¹,

Duan²

2022

WJCC

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“…Total DNA was extracted from the feces samples, as described previously (21). Briefly, 200 µL of fecal sample in GuSCN solution was lysed with glass beads (300 mg, 0.1 mm diameter) and 300 µL of lysis buffer (No.…”

Section: Dna Extractionmentioning

confidence: 99%

“…After DNA extraction, PCR amplification and DNA sequencing of the V3-V4 region of the bacterial 16S rRNA gene was performed in an Illumina MiSeq instrument (Illumina, San Diego, CA, USA) as described previously (21).…”

Section: Microbiome Analysismentioning

confidence: 99%

Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother–Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

et al. 2022

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Background: The gut microbiome and fecal metabolites of breastfed infants changes during lactation, and are influenced by breast milk components. This study aimed to investigate dynamic associations of milk components with the infant gut microbiome and fecal metabolites throughout the lactation period in a mother–infant model.Methods: One month after delivery, breast milk and subsequent infant feces were collected in a pair for 5 months from a mother and an exclusively breastfed infant. Composition of the fecal microbiome was determined with 16S rRNA sequencing. Low-molecular-weight metabolites, including human milk oligosaccharides (HMOs), and antibacterial proteins were measured in feces and milk using 1H NMR metabolomics and enzyme-linked immunosorbent assays. The association of milk bioactive components with the infant gut microbiome and fecal metabolites was determined with Python clustering and correlation analyses.Results: The HMOs in milk did not fluctuate throughout the lactation period. However, they began to disappear in infant feces at the beginning of month 4. Notably, at this time-point, a bifidobacterium species switching (from B. breve to B. longum subsp. infantis) occurred, accompanied by fluctuations in several metabolites including acetate and butyrate in infant feces.Conclusions: Milk bioactive components, such as HMOs, might play different roles in the exclusively breastfed infants depending on the lactation period.

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“…It has been reported that the absence of active α-defensin alters the composition of small intestinal microbiota in mice 14 , and oral administration of α-defensin rescues severe dysbiosis of graft-versus-host disease (GVHD) model mice 15 , indicating that α-defensin plays an important role in maintaining homeostasis of the intestinal microbiota. In addition, it has been known that α-defensin abnormalities cause dysbiosis and disruption of the intestinal metabolism in Crohn’s disease model mice 16 , 17 . Dysbiosis caused by Paneth cell damage with decreased α-defensin has been shown to relate to various diseases 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

Decrease of α-defensin impairs intestinal metabolite homeostasis via dysbiosis in mouse chronic social defeat stress model

Suzuki

Nakamura

Shimizu

et al. 2021

Sci Rep

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Psychological stress has been reported to relate to dysbiosis, imbalance of the intestinal microbiota composition, and contribute to the onset and exacerbation of depression, though, underlying mechanisms of psychological stress-related dysbiosis have been unknown. It has been previously established that α-defensins, which are effector peptides of innate enteric immunity produced by Paneth cells in the small intestine, play an important role in regulation of the intestinal microbiota. However, the relationship between disruption of intestinal ecosystem and α-defensin under psychological stress is yet to be determined. Here we show using chronic social defeat stress (CSDS), a mouse depression model that (1) the exposure to CSDS significantly reduces α-defensin secretion by Paneth cells and (2) induces dysbiosis and significant composition changes in the intestinal metabolites. Furthermore, (3) they are recovered by administration of α-defensin. These results indicate that α-defensin plays an important role in maintaining homeostasis of the intestinal ecosystem under psychological stress, providing novel insights into the onset mechanism of stress-induced depression, and may further contribute to discovery of treatment targets for depression.

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Disease progression-associated alterations in fecal metabolites in SAMP1/YitFc mice, a Crohn’s disease model

Cited by 11 publications

References 38 publications

Role of metabolites derived from gut microbiota in inflammatory bowel disease

Role of metabolites derived from gut microbiota in inflammatory bowel disease

Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother–Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

Decrease of α-defensin impairs intestinal metabolite homeostasis via dysbiosis in mouse chronic social defeat stress model

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