Disease-Free Survival of Colorectal Cancer Patients in Relation to CDw75 Antigen Expression

Villar-Portela, Susana; Muinelo‐Romay, Laura; Cuevas, Elisa; Gil‐Martín, Emilio; Fernández-Briera, Almudena

doi:10.1159/000326768

Cited by 2 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although this epitope is more abundant in the tumor tissue from CRC patients and its expression levels seem to correlate with the development of the neoplasia, the fact that most colorectal tumors express the molecule implies that CDw75 expression has a poor prognostic value (23). Additionally, it is unknown if the greater expression of the epitope observed in tumor tissue is a genuine molecular characteristic of CRC or just an epiphenomenon accompanying the malignization of the colorectal mucosa and, therefore, a poor discriminative marker.…”

Section: Discussionmentioning

confidence: 99%

“…The CDw75 antigen is expressed in the majority of colorectal tumors, where its expression is augmented with regard to the healthy mucosa (21,22). Additionally, CDw75 expression is particularly high in non-polypoid tumors and advanced or metastatic stages of the disease, although results from clinicopathological studies suggest it could represent a good prognosis marker for 3-year overall survival (23). However, the expression of CDw75 in tumor tissue from CRC patients showed no prognostic potential for predicting disease-free survival (23).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of proteins with the CDw75 epitope in human colorectal cancer

2017

View full text Add to dashboard Cite

Abstract. The CDw75 epitope is an α(2,6) sialylated antigen overexpressed in colorectal cancer (CRC), where its expression correlates with the progression of the disease. The CDw75 epitope is located mainly in N-glycoproteins, whose identity remains unknown. The aim of the present study was to identify proteins with the CDw75 epitope as a strategy to deepen the understanding of molecular pathogenesis of CRC and to identify novel biomarkers for this disease. For this purpose, a two-dimensional electrophoresis approach was employed. Protein spots in the gels were matched to the corresponding CDw75 positive spots in the immunoblotted polyvinylidene difluoride membranes, and further identification of the protein species was performed by mass spectrometry. Additionally, one-dimensional western blotting experiments were performed to verify the expression of these candidate proteins in the colorectal tissue and their coincidence in molecular mass with the CDw75-positive bands. The findings of the present study indicate that haptoglobin and the keratins 8 (K8) and 18 (K18) are proteins with the CDw75 epitope in the colorectal tissue from CRC patients and also suggest novel functions and cellular locations for these proteins in the colorectal tissue and in relation to CRC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of proteins with the CDw75 epitope in human colorectal cancer

2017

View full text Add to dashboard Cite

show abstract

Machine Learning-Based Integration Identifies Plasma Cells-Related Gene Signature ST6GAL1 in Idiopathic Pulmonary Fibrosis and Its Pan-Cancer Analysis

Lin,

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fibrotic disease that occurs primarily in older adults, and it is associated with a poor prognosis. Plasma cells are an essential effector cell in IPF development and progression. The aim of this study is to identify biomarkers associated with plasma cells in IPF and initially explore their potential role in the tumor immune microenvironment. Methods: The plasma cells marker genes were extracted via single cell RNA sequencing (scRNA-seq) analysis. Then, gene co-expression networks were generated to determine the hub genes most relevant to the IPF state and plasma cells infiltration level. Moreover, the differential expressed genes (DEGs) screening were conducted based on the bulk RNA-seq and microarray data. In addition, a machine learning-based integrative procedure for constructing a concordance plasma cells-related gene signature (PCRGS) were developed. Furthermore, a core gene in the PCRGS was identified and validated through experiments. Finally, the molecular docking procedure and pan-cancer analysis for the core gene were produced. Results: The established PCRGS based on the seven most potent genes was found to be an independent prognostic factor for overall survival and exhibited superior and robust performance when compared with conventional clinical features and 22 published signatures. Additionally, the PCRGS effectively distinguish IPF patients and normal subjects. Eventually, ST6GAL1 was selected as the core gene and its localization in the plasma cells as well as over-expression in the lungs of bleomycin-injured mice were validated. Three drugs related to ST6GAL1 were predicted, and the results showed that quercetin and ST6GAL1 might have a more stable binding conformation based on the molecular docking procedure. Furthermore, the results of a pan-cancer analysis confirmed a tight association between the ST6GAL1 expression and the prognosis of various tumors. Conclusions: PCRGS is an effective prognostic and predictive biomarker in IPF associated with immune responses, among which ST6GAL1 is a potential therapeutic target in diverse cancers and may act as a pivotal gene connecting IPF and cancer in terms of plasma cells immune effects.

show abstract

Disease-Free Survival of Colorectal Cancer Patients in Relation to CDw75 Antigen Expression

Cited by 2 publications

References 53 publications

Identification of proteins with the CDw75 epitope in human colorectal cancer

Identification of proteins with the CDw75 epitope in human colorectal cancer

Machine Learning-Based Integration Identifies Plasma Cells-Related Gene Signature ST6GAL1 in Idiopathic Pulmonary Fibrosis and Its Pan-Cancer Analysis

Contact Info

Product

Resources

About