Disease alleviating effects following prophylactic lemon and coriander essential oil treatment in mice with acute campylobacteriosis

Abstract

IntroductionGiven the worldwide increasing prevalence of human Campylobacter jejuni infections and the emergence of multi-drug resistant enteropathogenic strains, antibiotic-independent approaches applying non-toxic natural compounds for the treatment and prophylaxis of campylobacteriosis appear utmost desirable. In our placebo-controlled intervention study, we surveyed potential disease-alleviating including anti-pathogenic and immune-modulatory effects upon prophylactic oral application of lemon-essential oil (LEM-EO) and coriander-essential oil (COR-EO) in acute experimental campylobacteriosis.MethodsTherefore, secondary abiotic IL-10−/− mice were orally challenged with either LEM-EO or COR-EO starting seven days prior to peroral C. jejuni infection.Results and discussionSix days post-infection, slightly lower pathogen loads were assessed in the colon of mice from the LEM-EO as opposed to the COR-EO cohort if compared to placebo counterparts. Prophylactic application of both EOs improved the clinical outcome of acute campylobacteriosis which was paralleled by less distinct pathogen-induced colonic epithelial cell apoptosis. Moreover, mice subjected to LEM-EO and COR-EO prophylaxis displayed lower colonic numbers of macrophages/monocytes and of T lymphocytes, respectively, whereas in both verum groups, basal IL-6 and IFN-γ concentrations were measured in mesenteric lymph nodes on day 6 post-infection. The oral challenge with either EOs resulted in diminished secretion of distinct pro-inflammatory mediators in the kidney as well as serum samples derived from the infected mice. In conclusion, the results from our preclinical in vivo study provide evidence that LEM-EO and COR-EO constitute promising prophylactic measures to prevent severe campylobacteriosis which may help to reduce the risk for development of post-infectious sequelae in C. jejuni infected individuals.

Not only for Christmas: Prophylactic oral application of trans-cinnamaldehyde alleviates acute murine campylobacteriosis

“…[ 27 , 28 ]. Our previous studies addressing the disease-alleviating effects of urolithin-A [ 31 ], of activated charcoal [ 32 ], of the iron-chelating compound deferoxamine [ 33 ], of essential oils derived from garlic [ 34 ], cardamom [ 35 ], cumin [ 36 ], clove [ 37 ], coriander, and lemon [ 38 ], of phenolic compounds such as carvacrol [ 39 ], resveratrol [ 40 ], and curcumin [ 41 ], of organic acids including butyrate, benzoate, caprylate, and sorbate [ 42 , 43 ], and furthermore, of the vitamins ascorbate [ 44 ] and vitamin D [ 45 ] have underlined the suitability of the here applied secondary abiotic IL-10 −/− mice as preclinical setting to test antibiotics-independent therapeutic and preventive measures in the combat of campylobacteriosis. In the present placebo-controlled intervention trail we therefore surveyed the disease-alleviating effects of prophylactic oral tran s-cinnamaldehyde application in secondary abiotic IL-10 −/− mice starting a week prior oral C. jejuni infection.…”