Discussion by Earl R. Crouch, Jr., MD, Eric R. Crouch, MD

Crouch, Earl R.; Crouch, Eric R.

doi:10.1016/s0161-6420(02)01092-8

Cited by 16 publications

(19 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of the foresaid reasons, Nei's gene diversity value for CGE analysis was low compared to CVGE analysis. This is in accordance with Crouch et al [39], who stated that genetic divergence was found to be quite low within the plantain ecotypes gene pool, which is consistent with the proposed evolution of this germplasm through somatic mutation of a relatively small number of introductions.…”

Section: Genetic Diversity Within Populationsupporting

confidence: 93%

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

et al. 2019

View full text Add to dashboard Cite

Inter simple sequence repeat markers were employed for the genotyping of 16 plantain ecotypes. Two different electrophoretic systems namely conventional gel electrophoresis (CVGE) and fully automated high‐resolution CGE were used to evaluate the genetic diversity. Comparative analysis indicated that all parameters related to marker informativeness were higher in CGE except polymorphic information content. But genetic diversity parameters like effective number of alleles, Nei's gene diversity (1973) and Shannon's information index showed higher values (1.52 ± 0.12, 0.34 ± 0.05 and 0.52 ± 0.05, respectively) in CVGE as against CGE (1.29 ± 0.04, 0.22 ± 0.02 and 0.38 ± 0.03, respectively) system. The unweighed pair group method with arithmetic averages was used to obtain the dendrogram for both analyses. The results of dendrogram and principal component analysis were found to be consistent in both systems except for some minor disagreements. The clone‐specific bands could be used in the identification and development of SCAR markers. Inter simple sequence repeat markers used in this study provided sufficient polymorphism and reproducible banding pattern for evaluating the genetic diversity of different plantain ecotypes. Lack of accuracy and consistency of the CVGE warrants the employment of high‐throughput CGE for diversity analysis as it provided better separation of bands with higher resolution.

show abstract

Section: Genetic Diversity Within Populationsupporting

confidence: 93%

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The IMPRESS/selHMBC approach is described separately. 21,22 The selHMQCTOCSY, like selHMQC, is a band-selective variant of the gradient-based HMQCTOCSY experiment. The pulse sequence is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Increasing spectral information with shorter acquisitions: the advantages of using F₁ selection in structural determinations

Yang¹,

Gard²,

Harrigan³

et al. 2002

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

The structural elucidation of complex materials by NMR is complicated by the common occurrence of nearly coincident resonances. Congested regions necessitate increased spectral resolution, with additional time increments needed for 2D NMR spectroscopy, resulting in longer acquisition times. These demands conflict with the ever-increasing demands for instrument time and with long-term sample stability. Selective spectroscopy addresses both of these problems. By focusing attention only on the region of interest, fewer increments are needed. Shorter acquisitions are obtained, with a higher information content. Alternatively, the spectroscopist may choose to use these time savings to collect additional transients, allowing the study of smaller amounts of material, or low-level impurities/isomers. Using a carbohydrate as our example, we illustrate the utility of this methodology using selHMQC, selHMBC (IMPRESS) and selHMQCTOCSY. We have found this methodology useful in the coupling of efficiency and productivity of NMR spectroscopy.

show abstract

“…RNase H enzymes are widely distributed among prokaryotes and eukaryotes (24), and it is believed that they participate in DNA replication processes by removing the upstream RNA primers of Okazaki fragments (25). During the retrotransposition event of retrovirus and retrovirus-like elements an RNase H activity encoded by its own elements removes the RNA template of the generated RNA/cDNA hybrid in order to allow second strand DNA synthesis.…”

Section: Discussionmentioning

confidence: 99%

The Non-LTR (Long Terminal Repeat) Retrotransposon L1Tc fromTrypanosoma cruzi Codes for a Protein with RNase H Activity

Olivares¹,

García‐Pérez²,

Thomas³

et al. 2002

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Discussion by Earl R. Crouch, Jr., MD, Eric R. Crouch, MD

Cited by 16 publications

References 12 publications

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

Increasing spectral information with shorter acquisitions: the advantages of using F₁ selection in structural determinations

The Non-LTR (Long Terminal Repeat) Retrotransposon L1Tc fromTrypanosoma cruzi Codes for a Protein with RNase H Activity

Contact Info

Product

Resources

About

Discussion by Earl R. Crouch, Jr., MD, Eric R. Crouch, MD

Cited by 16 publications

References 12 publications

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers

Increasing spectral information with shorter acquisitions: the advantages of using F1 selection in structural determinations

The Non-LTR (Long Terminal Repeat) Retrotransposon L1Tc fromTrypanosoma cruzi Codes for a Protein with RNase H Activity

Contact Info

Product

Resources

About

Increasing spectral information with shorter acquisitions: the advantages of using F₁ selection in structural determinations