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Hokin, Mabel R.; Jeffries, Graham H.; Magee, D. F.; Rothman, Stephen; Thorn, Niels A.; Webster, Peter

doi:10.9783/9781512800746-021

Cited by 21 publications

(28 citation statements)

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“…Inositol phosphates (IP) are products of the catabolism of phosphatidylinositol (PI) phosphates (Hokin & Hokin 1953). Drug-receptor interactions enhance this catabolism (Michell 1975;Berridge 1984).…”

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confidence: 99%

Evidence for spare α1-adrenoceptors for the accumulation of inositol phosphates in smooth muscle

Fox

Friedman

1987

Journal of Pharmacy and Pharmacology

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The accumulation of inositol phosphates (IP) in smooth muscle from rat vas deferens and caudal artery was maximally increased 3- to 4-fold in response to exposure of the tissues to 100 microM noradrenaline. Clonidine (up to 3 mM) was a partial agonist. Pretreatment of the tissues with the irreversible alpha-adrenoceptor antagonist phenoxybenzamine (0.3-10 microM) shifted the noradrenaline concentration-response curve to the right before depressing the maximum. The maximum of the clonidine concentration-response curve was depressed without significant change in the EC50 by the same treatment. These data, which are most easily interpreted as demonstrating the presence of a receptor reserve for IP accumulation, are discussed.

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confidence: 99%

Evidence for spare α1-adrenoceptors for the accumulation of inositol phosphates in smooth muscle

Fox

Friedman

1987

Journal of Pharmacy and Pharmacology

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“…It was originally shown by the Hokins [1,2] that when slices of pancreatic exocrine tissue were stimulated to secrete digestive enzymes by the addition of acetylcholine there was a marked increase of 32Plabelling of the acidic phospholipids, especially phosphatidylinositol. An enhanced metabolism of acidic phospholipids has now been observed in a variety of situations in which cells are galvanized into heightened secretory or other activity by external stimulation L 4 1 .…”

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confidence: 99%

Inter-Relations between the Phospholipids of Rat Pancreatic Islets during Glucose Stimulation and Their Response to Medium Inositol and Tetracaine

1975

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1. Isolated rat pancreatic islets subjected to an increase in glucose concentration from 0.5 to 3 mg/ml showed an increased insulin secretion and "P-labelling of their phospholipids, especially of phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol, and CDP diglyceride. Neither fructose or inositol produced a similar effect.2. The enhanced labelling of CDP diglyceride and phosphatidylglycerol was suppressed by adding inositol (0.1 mg/ml), while the phosphatidylinositol labelling was increased still further.3. Tetracaine (0.5 mM), an antagonist of insulin secretion, inhibited phosphatidylinositol synthesis while phosphatidylglycerol, CDP diglyceride and phosphatidic acid formation were markedly increased. These effects on phospholipid synthesis were substantially reversed by adding inositol to the medium. Tetracaine also prevented the catabolism of phosphatidylinositol in prelabelled islets but this inhibition was not reversed by inositol. 4.Inositol addition did not affect insulin secretion in glucose-stimulated islets nor secretion in tetracaine-treated islets. This need not exclude a possible role for heightened phosphatidylinositol cleavage in stimulated insulin secretion.It was originally shown by the Hokins [1,2] that when slices of pancreatic exocrine tissue were stimulated to secrete digestive enzymes by the addition of acetylcholine there was a marked increase of

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“…Finally, experiments by Hokin et al (51) and Hokin (52) have implicated the phospholipids in membrane activities by showing that neurohormones such as acetyl choline and norepinephrine will stimulate the incorporation of 32PO43-into phospholipids. A skillfully executed experiment by Larrabee (53) provides support for the studies of Hokin and has provided possible physiological relevance.…”

Section: Influence Of Hormones and Drugsmentioning

confidence: 99%

Factors affecting incorporation of precursors into body constituents: A review of common sense considerations with glycolipids as examples

Burton

1970

Lipids

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The dynamics of cellular growth are of prime importance to the biochemist. The dynamic state of lipids can be studied by employing radioactive substrates or stable isotope‐labeled substrates. This paper illustrates major factors which may effect the incorporation of precursor substances into body constitutents. These factors are: (a) age and species; (b) molar size of the body's pool of precursor; (c) metabolic activity of the lipid being synthesized; (d) metabolic pathways; (e) route of administration of the precursor; (f) the nature of the precursor, i. e., molecular size, ionic or nonionic, water soluble vs. lipid solubility, and micelle formation; and (g) the influence of hormones and drugs. The synthesis and turnover of cerebrosides and gangliosides in the rat are used to illustrate these seven factors.

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Discussion

Cited by 21 publications

References 0 publications

Evidence for spare α1-adrenoceptors for the accumulation of inositol phosphates in smooth muscle

Evidence for spare α1-adrenoceptors for the accumulation of inositol phosphates in smooth muscle

Inter-Relations between the Phospholipids of Rat Pancreatic Islets during Glucose Stimulation and Their Response to Medium Inositol and Tetracaine

Factors affecting incorporation of precursors into body constituents: A review of common sense considerations with glycolipids as examples

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