Discriminatory ability of fractal and grey level co-occurrence matrix methods in structural analysis of hippocampus layers

Pantić, Igor; Đačić, Sanja; Brkić, Predrag; Lavrnja, Irena; Jovanović, Tatjana; Pantic, Senka; Peković, Sanja

doi:10.1016/j.jtbi.2015.01.035

Cited by 35 publications

(29 citation statements)

References 26 publications

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“…The fact that the FD did not demonstrate differences between the treatment groups and the injury group, can be associated with the fact of that method is not sensitive to the nuclei on the images. The works using FD and gray level co-occurrence matrix (GLCM), for hippocampus, corpus callosum and cingulum analysis (Pantic et al, 2015(Pantic et al, , 2014, GLCM alone or associated with FD had better results than the FD method alone, this improvement was attributed to the ability of the technique to quantify the density of the observed areas. In our findings, the extent of the injury area was smaller in the treated groups, resulting in denser inflammatory infiltrate.…”

Section: Discussionmentioning

confidence: 99%

Fractal dimension in the evaluation of different treatments of muscular injury in rats

et al. 2018

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Objectives: To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method. Methods: 35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method. Results: In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups. Conclusion: Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.

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Section: Discussionmentioning

confidence: 99%

Fractal dimension in the evaluation of different treatments of muscular injury in rats

et al. 2018

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“…Fractal analysis was done in FracLac (developed by A. Karperien, Charles Sturt University, Australia) (Karperien, –2007), a plugin of ImageJ software of the National Institutes of Health, (Bethesda, MD, USA) using conventional box‐counting method. For the details on this mathematical method, the reader is referred to our previously published works (Pantic et al ., ; Pantic et al ., ,b). Briefly, the nuclei were selected in ImageJ using freehand selection option.…”

Section: Methodsmentioning

confidence: 99%

Iron oxide nanoparticles decrease nuclear fractal dimension of buccal epithelial cells in a time‐dependent manner

Nikolovski

Dugalić

Pantić

2017

Journal of Microscopy

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In this paper, we present results that iron oxide nanoparticles (IONPs) induce time-dependent structural changes in nuclei of buccal epithelial cells. The cells were treated with magnetite, Fe O nanoparticles (spherical shape, diameter 80-100 nanometres). The digital micrographs of the nuclei were made in 3 different time points: 15, 30 and 60 min after the treatment with IONPs, as well as in the control cells. A total of 120 nuclear structures (30 per sample) were analysed. Fractal analysis of nuclei was done in ImageJ software of the National Institutes of Health, (Bethesda, MD, USA). For each nuclear structure, the values of fractal dimension and lacunarity were calculated. There was a time-dependent reduction of nuclear fractal dimension in buccal epithelial cells after exposure to magnetite iron oxide nanoparticles. Negative trend was observed (p < 0.01). Nuclear lacunarity, as another fractal parameter was shown to increase, also in a time-dependent manner, after the treatment with IONPs. To our knowledge, this is the first study to investigate effects of magnetite nanomaterial on nuclear fractal complexity, and also the first to apply fractal analysis method in testing of the interaction between nanoparticles and cell nucleus in this experimental setting.

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“…Analiza regiona od interesa je obavljena direktno sa mikrografa bez dodatne obrade poput kropovanja/ segmentovanja. Pojedinosti vezane za formiranje i obradu mikrografa (7,8) su prethodno objavljene.…”

Section: Materijal I Metode Protokol I Felgen Bojenjeunclassified

“…Teksturalni parametri su izračunati na sličan način kao u prethodno publikovanim radovima (7,8,10). GLCM analiza jedara je urađena korišćenjem programa za ImageJ u okviru MATLAB softverskog koda (MathWorks, Natick, Massachusetts, USA).…”

Section: Materijal I Metode Protokol I Felgen Bojenjeunclassified

The influence of lithium sulphate on Shannon entropy in lymphocyte chromatin

Jeremić¹,

Pantić²,

Jakšić³

2018

Medicinski podmladak

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Introduction: Lithium affects numerous signal pathways in cells, which may ultimately lead to either increased or decreased gene expression in cell nuclei. However, effects of lithium on higher level of gene organization in the nuclei, like chromatin, is still poorly understood.Aim: To investigate the effects of lithium on chromatin organization in the nuclei of lymphocytes by investigating changes in Shannon entropy of chromatin in these cells.Material and Methods: Peripheral blood was treated with lithium sulphate until lithium concentrations of 1mmol/l, 2 mmol/l i 3 mmol/l was not reached. The smears were fixed with methanol and stained by Felgen method for DNK visualization. Cells in the smear were treated with hydrochloric acid for 120 min. Schiff reagent was used for staining for 120 min and smear washing was done with 6 ml of 10% of water solution of natrium metabisulfite. After staining, digital micrographs for each of the most representative sample of 30 lymphocytic chromatin structures were made. Shannon entropy was calculated by converting micrograph in the format of textual numerical string. Lymphocyte chromatin was also analyzed by textural method analysis.Results: Treatment with 1 mmol/l of lithium sulphate did not lead to a statistically significant increase in entropy values (p > 0.05). However, in samples where the concentration of lithium was 2 i 3 mmol/l, respectively, there was a statistically significant increase in entropy (p < 0.05). Also, a statistically significant and dose-dependent linear trend of an increase of chromatin entropy was detected in samples (p < 0.05). After lithium sulphate treatment, neither the mean value of angular second moment lymphocyte chromatin in control sample nor the inverse moment of the difference of treated lymphocytes changed (p > 0.05).Conclusion: Lithium sulphate in peripheral blood lymphocytes causes dose-dependent increase in Shannon chromatin entropy, which is not followed with similar changes in a textural chromatin parameters.

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Discriminatory ability of fractal and grey level co-occurrence matrix methods in structural analysis of hippocampus layers

Cited by 35 publications

References 26 publications

Fractal dimension in the evaluation of different treatments of muscular injury in rats

Fractal dimension in the evaluation of different treatments of muscular injury in rats

Iron oxide nanoparticles decrease nuclear fractal dimension of buccal epithelial cells in a time‐dependent manner

The influence of lithium sulphate on Shannon entropy in lymphocyte chromatin

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