Discrimination of three BYDV species by one-step RT-PCR-RFLP and sequence based methods in cereal plants from the Czech Republic

Kundu, Jiban Kumar; Jarošová, Jana; Gadiou, S.; Červená, Gabriela

doi:10.1556/crc.37.2009.4.7

Cited by 21 publications

(18 citation statements)

References 18 publications

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“…In case of discordant results, a second test and a test with the reserve aphids sample were performed. The BYDV‐positive samples were further tested by RT‐PCR‐RFLP according to Kundu et al. (2009) so that the BYDV species could be identified.…”

Section: Methodsmentioning

confidence: 99%

“…BYDV‐PAS (type isolate PAV‐129) was recently separated from PAV (Mayo, 2002). As these two species were indistinguishable by serological methods, molecular diagnostic methods such as RT‐PCR‐RFLP (Kundu et al. , 2009) and sequencing were used to this end.…”

Section: Introductionmentioning

confidence: 99%

“…, 2009) and sequencing were used to this end. Sequence analysis of BYDV field isolates conducted in the Czech Republic revealed that BYDV‐PAS and ‐MAV were the most frequent isolates, whereas BYDV‐PAV seemed to be less frequent in cereal crops (Kundu et al. , 2009).…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of the Barley yellow dwarf virus species PAV and PAS: distribution, accumulation and host resistance

et al. 2012

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This study investigated the distribution and characteristics of the Barley yellow dwarf virus (BYDV) species BYDV-PAS, which was recently separated from BYDV-PAV, the most commonly studied BYDV species. Throughout 3 years of experimental monitoring of BYDV incidence, PAS was the most frequently occurring species infecting cereals and grasses in the Czech Republic. Furthermore, Rhopalosiphum maidis and Metopolophium dirhodum were recorded as BYDV-PAS vectors, even though M. dirhodum does not usually transmit BYDV-PAV. In field experiments with barley and wheat, where virus accumulation, symptoms and effect on the yield were tested, BYDV-PAV was more severe than PAS. Infection with the BYDV-PAV isolate resulted in greater expression of symptoms and also in a greater reduction in plant height and grain weight per spike than BYDV-PAS. In a sensitive cultivar of barley (Graciosa), the amount of viral RNA of BYDV-PAV was also significantly higher than that of BYDV-PAS. In a tolerant line (Wbon-123), however, no such differences were found. In conclusion, although BYDV-PAS seems to be dominant in the Czech Republic, BYDV-PAV has the potential to cause more significant crop losses in barley and wheat.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A comparative study of the Barley yellow dwarf virus species PAV and PAS: distribution, accumulation and host resistance

et al. 2012

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“…these haplotypes in different countries could be explained by migration of aphids having the same origin. Results of CP amino acid sequence analysis showed the presence of the conserved amino acid motif "PDSIPGS" that is usually present in BYDV-PAV-II isolates (Liu et al, 2007;Kundu et al, 2009).…”

Section: Fig 2 Phylogenetic Tree Of Bydv Isolates Based On 531 Nuclementioning

confidence: 99%

Molecular characterization of barley yellow dwarf virus in Tunisia

Bouallegue¹,

Mezghani-Khémakhem²,

Bouktila³

et al. 2014

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Barley yellow dwarf disease is a worldwide ubiquitous virus disease of cereal crops. In order to characterize the B/CYDV isolates occurring in Tunisia, 240 barley leaves were randomly sampled from 6 fields following a North-South trend and analyzed by serological and molecular tests. DAS-ELISA results showed 40 positive samples with a prevalence of barley yellow dwarf virus (BYDV)-PAV (77.5%), followed by cereal yellow dwarf virus (CYDV)-RPV (25%) and BYDV-MAV (15%). Studies of the geographic distribution showed a high incidence of B/CYDV in the Tunisian Southern provinces. RT-PCR assays were performed to amplify the viral coat protein gene (CP) and sequence analyses revealed six BYDV-PAV haplotypes named PAV-TN1 to PAV-TN6. Phylogenetic analysis showed that the six Tunisian haplotypes were close to BYDV-PAV-II subspecies and had a strong similarity with Moroccan, Czech, French and German haplotypes. Although PAV-TN2 and PAV-TN5 showed up to 10% divergence from BYDV-PAV-II at the amino acid level, it seems to belong to the same subspecies but in a separated cluster. Our results will be important in developing appropriate control measures against BYDV disease in Tunisia.

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“…BYDVs are ssRNA viruses belonging to the genera Luteovirus (BYDV strains PAV, PAS, MAV, and Kerll) and Polerovirus (cereal yellow dwarf virus-RPV, maize yellow dwarf virus-RMV) and of unassigned stains (BYDV-SGV and BYDV-GPV) in the family Luteoviridae (ICTV 2018). Strains PAV, PAS, and MAV of BYDV have been found in infected cereal crops and grasses in the Czech Republic [4], with a prevalence of PAV in crop species (wheat, barley) and PAS in volunteer cereals and grasses [5]. BYDVs are transmitted by more than 25 species of aphids [6].…”

Section: Introductionmentioning

confidence: 99%

The Evaluation of Wheat Cultivar Resistance and Yield Loss Thresholds in Response to Barley Yellow Dwarf Virus-PAV Infection

et al. 2020

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The PAV strain of barley yellow dwarf virus (BYDV) is one of the causal agents of yellow dwarf disease in cereals. The use of germplasm resistant to BYDV is generally regarded as the most effective means of controlling damage caused by this pathogen. In field trials, response to infection with a barley yellow dwarf virus of selected wheat cultivars registered in the Czech Republic was compared with that of control cultivars. Although a good level of resistance to BYDV-PAV was found in cultivar Athlon and yield loss was low, symptoms were more severe than on the moderately resistant control cultivar Sparta. Several other cultivars, such as Nordika, Julie, and Replik, also had slightly less than a 30% reduction in grain weight per spike, even though symptoms were more severe on Sparta or Athlon. Our results showed that, in the case of approximately 60% of wheat plants with BYDV-PAV symptoms, the yield reductions under optimal agronomic conditions reached approximately 17% for moderately resistant cultivars and 30% for moderately susceptible cultivars. The application of N fertilizer significantly reduced yield losses in BYDV-PAV-infected wheat cultivars, particularly in the moderately resistant cultivars. Even when infected with BYDV-PAV, the yield of moderately resistant cultivars, including those of spring wheat, was still acceptable. However, the re-cultivation costs of spring wheat in replacing damaged winter wheat lead to a total economic loss per hectare that is much greater than that for BYDV-infected wheat cultivars (moderately resistant and/or moderately susceptible ones). Furthermore, the economic loss is much lower when a moderately resistant cultivar is used. Hence, even with a high level of disease symptoms in winter wheat, the re-cultivation of spring wheat is not economically feasible.

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Discrimination of three BYDV species by one-step RT-PCR-RFLP and sequence based methods in cereal plants from the Czech Republic

Cited by 21 publications

References 18 publications

A comparative study of the Barley yellow dwarf virus species PAV and PAS: distribution, accumulation and host resistance

A comparative study of the Barley yellow dwarf virus species PAV and PAS: distribution, accumulation and host resistance

Molecular characterization of barley yellow dwarf virus in Tunisia

The Evaluation of Wheat Cultivar Resistance and Yield Loss Thresholds in Response to Barley Yellow Dwarf Virus-PAV Infection

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