Discrimination of Muscle Fiber Types in Ordinary Muscle by Actomyosin ATPase Activity and Its Comparison among Various Fishes and Muscle Parts

Comparison of Interposition Ratios of Pink Muscle Fiber in Dorsal Ordinary Muscle among Various Marine Fishes.

“…1 and 2). Findings similar to this have been reported in the grey mullet by Carpene et al (1982), the cod by Korneliussen et al (1978), the roach by Kilarski (1990), and a number of fish species by both Mosse & Hudson (1977), and our own previous studies (Jabarsyah et al, 1999a(Jabarsyah et al, , 1999b. The present study clarified that the number and area percentages of pink muscle fibers against all kinds of muscle fiber types in the dorsal ordinary muscle were considerably different among fish species, and fell within ranges of from 12.90% to 56.53% and from 2.22% to 53.68%, respectively (Fig.…”

“…We speculate that it may be connected with not only a difference in genes but also swimming ability, because the muscle fiber type in the dorsal ordinary muscle differed between the cultured and wild specimens of the red sea bream (Jabarsyah et al, 1999a), and metabolism is more aerobic in pink than in white muscle fiber. We reported in previous studies that the histochemical actomyosin ATPase activity of pink muscle fibers in the mosaic pattern of ordinary muscle was very stable in both acid and alkaline preincubations, which was similar to that of these fibers in the intermediate part of the muscle (Jabarsyah et al, 1999a), and also that the glycogen content in the pink muscle fibers in ordinary muscles was considerably higher, which was also similar to that in the intermediate part of the muscle (Yada et al, 2000). Namely, the histochemical characteristics of pink muscle fibers in both ordinary and intermediate muscles were very similar.…”

“…Korneliussen et al (1978) and Walesby and Johnston (1980) reported that a boundary region between the layers of intermediate muscle and ordinary muscle was not remarkable in the cod Gadus morhua, and that the Antarctic toleost Notothenia rosii had no pink muscle fiber in its locomotory muscles. In our previous study, we reported that there were not only white muscle fibers but also pink muscle fibers in the dorsal ordinary muscle of many fish species, and that the fiber type in this muscle was differed remarkably among species, between wild and cultured fish of the same species, and among regions along the length of the body and toward the neural spine (Jabarsyah et al, 1999a).…”

“…Korneliussen et al (1978) and Walesby and Johnston (1980) reported that a boundary region between the layers of intermediate muscle and ordinary muscle was not remarkable in the cod Gadus morhua, and that the Antarctic toleost Notothenia rosii had no pink muscle fiber in its locomotory muscles. In our previous study, we reported that there were not only white muscle fibers but also pink muscle fibers in the dorsal ordinary muscle of many fish species, and that the fiber type in this muscle was differed remarkably among species, between wild and cultured fish of the same species, and among regions along the length of the body and toward the neural spine (Jabarsyah et al, 1999a).We also reported that the presence of pink muscle fiber in the ordinary muscle raised the maximum level in rigor mortis in various species (Jabarsyah et al, 1999b), and accelerated the temporal change of K-value (one of the freshness indices) in the carp (Yada et al, 2000). However, the influence of muscle fiber type on the temporal change of K-value, especially by the presence or absence of pink muscle fiber in the ordinary muscle has not yet been examined thoroughly.…”

“…Functionally, the former is implicated in sustained slow swimming and the latter is in bursts of violent activity (Matsuoka & Iwai, 1984). The white muscle fibers are subdivided into Types IIa and IIb, the IIa subtype being used not only for anaerobic metabolism but also for aerobic metabolism, and the IIb subtype especially for anaerobic metabolism in response to typical escape and attack movement; the pink muscle fiber is more particularly for aerobic metabolism than the IIa subtype (Jabarsyah et al, 1999a). The pink muscle fiber is characterized as fast contracting with intermediate resistance to fatigue and intermediate speed of shortening between red and white muscle fibers (Johnston et al, 1977).…”