Discrimination of Influenza Virus Strains and Super High Sensitive Detection of Viruses Using Sugar Chip and Sugar-Chain Immobilized Gold Nanoparticles

Suda, Yasuo; Xu, Zhifeng; Takahashi, Yuko; Yokoyama, Risa; Nagatomo, Mami; Aoyama, Kazue; Okuno, Toshiomi; Saito, Shigeru; Morikawa, Saeko; Hiroi, Satoshi; Kase, Tetsuo; Murakami, Naoki; Nishi, Junichiro; Wakao, Masahiro

doi:10.1021/bk-2013-1135.ch020

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…Sugar-chain-immobilized particles can be expected to be useful in a wide variety of biomedical applications such as in the separation and purification of toxic proteins, pathogenic viruses, or bacteria, because sugar chains are known to adsorb these toxins [1][2][3][4]. By introducing fluorescent substances into the sugar-chain-immobilized particles, highly sensitive fluorescent detection methods are made possible.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a sugar-immobilized fluorescent PMMA shell on a Ni core particle via soap-free emulsion polymerization

et al. 2022

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Sugar chain immobilized polymer particles having both magnetic and fluorescent properties can be expected to be useful in a wide variety of biomedical applications such as the detection, separation, and purification of proteins, viruses, or bacteria, because sugar chains specifically adsorb them. Since high magnetic responsiveness is required for such applications, we attempted to fabricate core-shell particles consisting of a submicron-sized magnetic core and a thin polymer shell (nano-to dozens of nanometers thick) that incorporates a fluorescent dye, with sugar molecules immobilized on the surface. Soapfree emulsion polymerization using methyl methacrylate (MMA) monomer and potassium persulfate (KPS) initiator in the presence of aminopropyltrimethoxysilane-treated Ni particles, octyl-β-D-glucopyranoside (octyl-glc), and rhodamine B (RhB) produced a glucose-immobilized fluorescent PMMA thin shell on a Ni particle (Ni/PMMA/RhB/octyl-glc). Electrostatic interaction was used both to incorporate RhB into the PMMA shell and to coat the Ni core with the PMMA-RhB shell. Glucose was immobilized on the PMMA shell by embedding a hydrophobic octyl group derived from octyl-glc in the PMMA matrix, and the resulting sugar-immobilized PMMA shell was able to adsorb protein (concanavalin A; a protein that specifically adsorbs glucose). The resulting Ni/PMMA/RhB/octyl-glc particles were well-dispersed in water, detected by highly sensitive fluorescence techniques, and could be collected by a magnet within 10 sec. They are expected to be applied to detect biological substances such as various proteins and viruses by changing the glucose moiety of the particle surface to other functional glycans.

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Section: Introductionmentioning

confidence: 99%

Fabrication of a sugar-immobilized fluorescent PMMA shell on a Ni core particle via soap-free emulsion polymerization

et al. 2022

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“…Even though the capability of the Boom method has been improved by several companies including Qiagen, it still takes time and it also extracts and purifies free RNA of SARS-CoV-2 in specimens that have no infectious activity due to the loss of spike protein or envelope in the matured viral structure. We applied our nano-biotechnology, SGNP method [10][11][12][13][14], in which sugar chain-immobilized magnetized gold nanoparticles and micro-meter sized magnetic particles were used to collect and purify virions that have matured spike protein and viral particles. Then, by adding detergent solution to the collected mixture, viral RNAs were extracted from the purified virion, followed by applying the solution to qRT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients’ Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients

Kajiya¹,

Sawayama

Arima

et al. 2022

Viruses

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Background: The transmissible capacity and toxicity of SARS-CoV-2 variants are continually changing. We report here the follow-up study of hospitalized COVID-19 patients from 2020 to 2022. It is known that the PCR diagnosis for hospitalized patients sometimes causes confusion because of the incompatibility between their diagnosis and symptoms. We applied our sugar chain-immobilized gold-nanoparticles for the extraction and partial purification of RNA from specimens for quantitative RT-PCR assay and evaluated whether the results correlate with patients’ symptoms. Methods and Results: Saliva specimens were taken from hospitalized patients with mild or moderate symptoms every early morning. At the time of RT-PCR diagnosis, two methods for the extraction and partial purification of RNA from the specimen were performed: a commonly used Boom (Qiagen) method and our original sugar chain-immobilized gold nanoparticle (SGNP) method. For symptoms, body temperature and oxygen saturation (SpO2) of patients were monitored every 4 h. Conclusions: It was clear that patients infected with the Delta variant needed more time to recover than those with the Omicron variant, and that the SGNP method showed more realistic correlation with the symptoms of patients compared with the common Qiagen method.

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Diagnostic Method for COVID-19 Using Sugar Chain–Immobilized Nanoparticles and Saliva Specimens

Suda

Tajima

Nishi

et al. 2022

Methods in Molecular Biology

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Discrimination of Influenza Virus Strains and Super High Sensitive Detection of Viruses Using Sugar Chip and Sugar-Chain Immobilized Gold Nanoparticles

Cited by 8 publications

References 11 publications

Fabrication of a sugar-immobilized fluorescent PMMA shell on a Ni core particle via soap-free emulsion polymerization

Fabrication of a sugar-immobilized fluorescent PMMA shell on a Ni core particle via soap-free emulsion polymerization

Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients’ Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients

Diagnostic Method for COVID-19 Using Sugar Chain–Immobilized Nanoparticles and Saliva Specimens

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