2012
DOI: 10.1556/eujmi.2.2012.2.8
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Discrimination ofBurkholderia mallei/pseudomalleifromBurkholderia thailandensisby sequence comparison of a fragment of the ribosomal protein S21 (rpsU) gene

Abstract: Discrimination of Burkholderia (B.) pseudomallei and B. mallei from environmental B. thailandensis is challenging. We describe a discrimination method based on sequence comparison of the ribosomal protein S21 (rpsU) gene. The rpsU gene was sequenced in ten B. pseudomallei, six B. mallei, one B. thailandensis reference strains, six isolates of B. pseudomallei, and 37 of B. thailandensis. Further rpsU sequences of six B. pseudomallei, three B. mallei, and one B. thailandensis were identified via NCBI GenBank. Th… Show more

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Cited by 8 publications
(20 citation statements)
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“…Recently, sequence analysis of a 120-base-pair fragment of rpsU coding for a ribosomal protein S21 homolog with a length of 70 amino acids upstream of the B. pseudomallei fl iC [25] was described as a method for discriminating B. mallei and B. pseudomallei from apathogenic B. thailandensis within the B. pseudomallei complex [26]. The protein belongs to the eubacterial macromolecular synthesis (MMS) operon playing a role in the initiation of protein, DNA, and RNA synthesis.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Recently, sequence analysis of a 120-base-pair fragment of rpsU coding for a ribosomal protein S21 homolog with a length of 70 amino acids upstream of the B. pseudomallei fl iC [25] was described as a method for discriminating B. mallei and B. pseudomallei from apathogenic B. thailandensis within the B. pseudomallei complex [26]. The protein belongs to the eubacterial macromolecular synthesis (MMS) operon playing a role in the initiation of protein, DNA, and RNA synthesis.…”
Section: Introductionmentioning
confidence: 99%
“…The protein belongs to the eubacterial macromolecular synthesis (MMS) operon playing a role in the initiation of protein, DNA, and RNA synthesis. The species-specifi c variations within the Burkholderia genus seem to be low [26,27]. So far, the power of rpsU sequencing to discriminate agents of the B. pseudomallei complex from other Burkholderia spp.…”
Section: Introductionmentioning
confidence: 99%
“…Focusing on combined approaches for the detection of B. pseudomallei and B. mallei , the causative agents of melioidosis and glanders, respectively [1, 2], Ashdown agar was described to be most sensitive for the isolation of B. pseudomallei [25]. The finding of that study [25] that growth of B. mallei generally fails on this medium, however, could not be confirmed by the here presented data using Ashdown + G agar, although the agar did not allow the growth of all B. mallei strains investigated.…”
Section: Discussionmentioning
confidence: 99%
“…mallei , the causative agent of glanders, and Burkholderia pseudomallei , the causative agent of melioidosis [1, 2], species with relevance for cystic fibrosis patients, e.g., the Burkholderia cepacia complex, and environmental species [3, 4]. …”
Section: Introductionmentioning
confidence: 99%
“…Several methods have been developed for detection of various combinations of these species, which include serologic tests (Samosornsuk et al, 1999;Steinmetz et al, 1999;Anuntagool et al, 2000;Chenthamarakshan et al, 2001;Cheng et al, 2006), commercial biochemical tests (Inglis et al, 1998;Lowe et al, 2002;Glass and Popovic, 2005;Amornchai et al, 2007), GC-FAME (Inglis et al, 2003), GLC-FAME (Inglis et al, 2005), microscopic methods (Walsh et al, 1994;Wuthiekanun et al, 2005;Hagen et al, 2011), MALDI-TOF (Inglis et al, 2012), PCR subtractive hybridization technique , PCR-RFLP (Tanpiboonsak et al, 2004) and gene sequencing (Woo et al, 2002;Gee et al, 2003;Frickmann et al, 2012). Serologic tests may be unreliable in endemic areas due to seroconversion (White, 2003) for those previously exposed to the organism.…”
Section: B Pseudomallei and Melioidosismentioning
confidence: 99%