Discrimination of different generations of Zeugodacus scutellata using age grading technique and their local genetic variation

Kim, Yonggyun; Baki, Abdullah Al; Kwon, Gimyeon; Kim, Dongsoon; Park, Kye Chung; Ahn, Jeong Joon

doi:10.1016/j.aspen.2019.07.010

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…gDNA was extracted as described above. Two RAPD primers, N-8017 (5 -TGCTCTGCCC-3 ) and N-8041 (5 -ATCGGGTCCG-3 ) [18], were used for genotyping of individual thrips. PCR reaction mixture (25 µL) consisted of 2 µL of gDNA, 2.5 µL of 10× PCR buffer, 2.5 µL of dNTP, 2 µL of a single RAPD primer, 1 µL of Taq DNA polymerase (GeneAll), and 15 µL of deionized distilled water.…”

Section: Random Amplified Polymorphic Dna (Rapd) Analysis Of Local Populationsmentioning

confidence: 99%

Thelytokous Reproduction of Onion Thrips, Thrips tabaci Lindeman 1889, Infesting Welsh Onion and Genetic Variation among Their Subpopulations

Khan

Roy

Kim

2022

Insects

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Parthenogenesis is not uncommon in thrips. This asexual reproduction produces males (arrhenotokous) or female (thelytokous). Only females are found in the onion thrips (Thrips tabaci Lindeman 1889) infesting Welsh onion (Allium fistulosum) in several areas of Korea. To determine the reproduction mode of T. tabaci, thrips infesting Welsh onion were collected from different localities in Korea. Cytochrome oxidase I (COI) sequences were then assessed. Results showed that all test local populations had signature motif specific to a thelytokous type. These COI sequences were clustered with other thelytokous populations separated from arrhenotokous T. tabaci populations. In a laboratory test, individual rearing produced female progeny without any males. These results support that Korean onion thrips infesting Welsh onion have the thelytokous type of parthenogenesis. Local thrips populations exhibited significant variations in susceptibility to chemical and biological insecticides. Random amplified polymorphic DNA (RAPD) analysis indicated genetic variations of local populations. However, the genetic distance estimated from RAPD was independent of the actual distance among different local populations. These results suggest that genetic variations of T. tabaci are arisen from population subdivision due to asexual thelytokous reproductive mode.

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Section: Random Amplified Polymorphic Dna (Rapd) Analysis Of Local Populationsmentioning

confidence: 99%

Thelytokous Reproduction of Onion Thrips, Thrips tabaci Lindeman 1889, Infesting Welsh Onion and Genetic Variation among Their Subpopulations

Khan

Roy

Kim

2022

Insects

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“…Identifying new ligands that are likely to exist could serve as helpful pest management targets, emphasizing the significance of understanding this underlying biology, particularly for applied sterile insect technique (SIT) approaches. Kim et al (2020) used electron beam irradiation to develop SIT in Z. scutellata . When male pupae were exposed to 200 Gy, they were developed and mated with nonirradiated female.…”

Section: Introductionmentioning

confidence: 99%

Insights on reproduction‐related genes in the striped fruit fly, Zeugodacus scutellata (Hendel) (Diptera: Tephritidae)

Gokulanathan,

Mo,

Park

2023

Arch Insect Biochem Physiol

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The striped fruit fly, Zeugodacus scutellata is a significant pest in East and Southeast Asia by damaging Cucurbitaceae blossoms and fruits. To control this pest, a novel strategy to suppress the gene(s) associated with sexually dimorphic phenotypes has been devised and implemented in a laboratory scale. However, comprehensive transcriptomic analysis related to this sex differentiation of Z. scutellata was necessary to determine effective target genes for the genetic control. We performed de novo assembly of the transcript obtained by paired‐end sequencing using an Illumina HiSeq platform and let to 217,967 unigenes (i.e., unique genes) with a minimum length of 200 bp. The female produced 31, 604, 442 reads with 97.93% of Q20, 94.76% of Q30, and the male produced 130, 592, 828 reads with 97.93% of Q20 and 94.76 of Q30%. The differentially expressed genes were used to predict genetic factors associated with sex differentiation, which included Rho1, extra‐macrochaetae (emc), hopscotch (hop), doublesex (dsx), sex‐lethal (sxl), transformer‐2 (tra‐2), testis‐specific serine/threonine‐protein kinase (tssk1), tektin1 (tkt1) and 2 (tkt2), odorant binding proteins (OBPs), fruitless (fru), vitellogenin receptor, and hormone receptors in Z. scutellata. In addition, this transcriptome analysis provides the additional gene associated with sex determination and mating behaviors, which would be applied to develop a novel sterile insect technique against Z. scutellata.

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Discrimination of different generations of Zeugodacus scutellata using age grading technique and their local genetic variation

Cited by 2 publications

References 19 publications

Thelytokous Reproduction of Onion Thrips, Thrips tabaci Lindeman 1889, Infesting Welsh Onion and Genetic Variation among Their Subpopulations

Thelytokous Reproduction of Onion Thrips, Thrips tabaci Lindeman 1889, Infesting Welsh Onion and Genetic Variation among Their Subpopulations

Insights on reproduction‐related genes in the striped fruit fly, Zeugodacus scutellata (Hendel) (Diptera: Tephritidae)

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