Discrimination of colorectal cancer cell lines using microwave biosensors

Zhang, L.Y.; Puch, Christophe Bounaix Morand Du; Dalmay, Claire; Lacroix, Aurélie; Landoulsi, Alaeddine; Leroy, Jonathan; Mélin, Carole; Lalloué, Fabrice; Battu, Serge; Lautrette, Christophe; Giraud, Stéphanie; Bessaudou, Annie; Blondy, Pierre; Jauberteau, Marie‐Odile; Pothier, A.

doi:10.1016/j.sna.2014.03.022

Cited by 48 publications

(24 citation statements)

References 30 publications

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“…The requirements for the sensing precision expressed in terms of the minimum detectable capacitance are determined as follows. According to [11], there is approximately 10% difference in the relative permittivity between the colorectal cancer cells at different aggressive stages. These percentage differences in permittivity translate to a capacitance change of 16 aF for cells with a diameter of 10 µm.…”

Section: Signal Analysis and System Requirementmentioning

confidence: 99%

“…Assuming the bandwidth of noise filtering is much smaller than that of injection locking, the minimum detectable frequency shift normalized to ω 0 can be derived by dividing (12) to (7) with (11) Δω ω 0 2 n,rms,ILO…”

Section: Sensor Noise Analysismentioning

confidence: 99%

“…This motivates the investigation of using microwave signature as effective discrimination between cells of different phenotypes based on the size and the morphology, the number of organelles, the volumetric ratio between the nucleus and cytoplasm, and the amount of bound and bulk water content [9], [11]. In the past, such study is carried out with large numbers of cells suspended in aqueous medium [10]; it is not until recently that [11] demonstrates there is a significant difference (∼10%) in permittivity between colorectal cancer cells at different aggressive stages at 5-15 GHz. Nevertheless, these measurements are carried out by the dehydration of the medium after loading the cells onto a MEMS resonator, leading to potential alteration of its physiological status.…”

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confidence: 99%

See 2 more Smart Citations

Oscillator-Based Reactance Sensors With Injection Locking for High-Throughput Flow Cytometry Using Microwave Dielectric Spectroscopy

Chien

Niknejad

2016

IEEE J. Solid-State Circuits

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This paper presents the analysis and design of oscillator-based reactance sensors employing injection locking for high-throughput label-free single-cell analysis using dielectric spectroscopy at microwave frequencies. By injection-locking two sensing LC-oscillators with an I/Q excitation source, the measurement of the sample-induced frequency shift caused by the interaction with the electromagnetic fields is performed through phase detection with injection-strength-dependent transducer gain. Such inherent phase amplification offered by the injection locking not only relaxes the design requirement for the readout circuits but also maintains the highest rejection against common-mode errors associated with the drift of the supply voltage and the environmental parameters. To reduce flicker noise contribution, a chopping technique employing phase modulation is exploited. In addition, this paper presents a novel ping-pong chopping approach to alleviate chopping-induced dc offset. In this prototype, four sensing channels, covering frequencies between 6.5 and 30 GHz, are distributed along a microfluidic channel fabricated with standard photolithography. Measurements show that the proposed microwave capacitive sensors achieve a sub−aFrms of noise sensitivity at 100 kHz filtering bandwidth, enabling measurement throughput exceeding 1 k cells/s. The sensor prototype is implemented in 65 nm CMOS technology and consumes 65 mW at 1 V supply.

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Section: Signal Analysis and System Requirementmentioning

confidence: 99%

Section: Sensor Noise Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Oscillator-Based Reactance Sensors With Injection Locking for High-Throughput Flow Cytometry Using Microwave Dielectric Spectroscopy

Chien

Niknejad

2016

IEEE J. Solid-State Circuits

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“…Compared to the other microwave IDC (Interdigitated Coplanar Waveguides) based sensors the current reflectometer demonstrates single cell sensitivity without the need for a bulky Vector Network Analyzer (VNA) [21][22][23]. The sensor functions by detecting electrical length changes induced in interdigitated coplanar transmission lines by presence of a cell.…”

Section: Introductionmentioning

confidence: 99%

A compact microwave frequency reflectometer with attoFarad sensitivity: A path towards an integrated dielectrophoresis cytometer

Mohammad

Braasch

Salimi

et al. 2015

Sensors and Actuators A: Physical

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“…It is especially critical in broadband detection for which the signal-to-noise ratio is usually much lower than that of narrowband resonance [10] or interference [11] techniques. Despite these challenges, broadband electrical detection at or near the single-cell level has recently been demonstrated [12], [13].…”

mentioning

confidence: 99%

Broadband Electrical Detection of Individual Biological Cells

Ning

Multari

Luo

et al. 2014

IEEE Trans. Microwave Theory Techn.

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To resolve the dilemma of cell clogging or solution parasitics encountered by Coulter counters and to evolve a general-purpose electrical detection technique, we used broadband microwave measurements to overcome electrode polarization, ac dielectrophoresis to precisely place cells between narrowly spaced electrodes, and relatively wide microfluidic channels to prevent cell clogging. This unique combination of approaches resulted in reproducible sensing of single Jurkat and HEK cells, both live and dead, of different cultures at different times.

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Discrimination of colorectal cancer cell lines using microwave biosensors

Cited by 48 publications

References 30 publications

Oscillator-Based Reactance Sensors With Injection Locking for High-Throughput Flow Cytometry Using Microwave Dielectric Spectroscopy

Oscillator-Based Reactance Sensors With Injection Locking for High-Throughput Flow Cytometry Using Microwave Dielectric Spectroscopy

A compact microwave frequency reflectometer with attoFarad sensitivity: A path towards an integrated dielectrophoresis cytometer

Broadband Electrical Detection of Individual Biological Cells

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