Discrimination of Candida species by paper spray mass spectrometry

Hamid, Ahmed; Pu, Weihua; Jarmusch, Alan K.; Pirro, Valentina; Cooks, R. Graham

doi:10.1016/j.ijms.2014.09.013

Cited by 26 publications

(14 citation statements)

References 70 publications

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“…In this work the lipid profile of bacteria colonies grown on agar was used in combination with multivariate data analysis to identify species of gram positive bacteria with a 98% success rate and of gram negative bacteria with a success rate of 87%. In similar work by the same group, eight different species of Candida were differentiated at the species level based on their lipid profile with similar levels of success [35]. Applications related to microorganisms aren't limited to species identification.…”

Section: B Biomoleculesmentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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Paper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations. _________________________________________________________________________________ AbstractPaper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations.

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Section: B Biomoleculesmentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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“…Other applications include analysis of agrochemicals and contaminants in food [15][16][17], tissue analysis [18], surface swabbing [19,20], profiling of algae [21] and bacteria [22,23], cell culture monitoring [24], and herbicides [25] in water and crop extracts. In addition to applications development, methodological advancements have been reported for paper spray MS such as improving selectivity using differential mobility spectrometry prior to MS detection [26], improving sensitivity using solid phase extraction [27], the use of alternative porous substrates [7,[28][29][30], and using paper spray as an ionization source for microfluidics chips [31,32].…”

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confidence: 99%

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Vega

Spence

Zhang

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Paper spray mass spectrometry is a method for the direct analysis of biofluid samples in which extraction of analytes from dried biofluid spots and electrospray ionization occur from the paper on which the dried sample is stored. We examined matrix effects in the analysis of small molecule drugs from urine, plasma, and whole blood. The general method was to spike stable isotope labeled analogs of each analyte into the spray solvent, while the analyte itself was in the dried biofluid. Intensity of the labeled analog is proportional to ionization efficiency, whereas the ratio of the analyte intensity to the labeled analog in the spray solvent is proportional to recovery. Ion suppression and recovery were found to be compound-and matrix-dependent. Highest levels of ion suppression were obtained for poor ionizers (e.g., analytes lacking basic aliphatic amine groups) in urine and approached -90%. Ion suppression was much lower or even absent for good ionizers (analytes with aliphatic amines) in dried blood spots. Recovery was generally highest in urine and lowest in blood. We also examined the effect of two experimental parameters on ion suppression and recovery: the spray solvent and the sample position (how far away from the paper tip the dried sample was spotted). Finally, the change in ion suppression and analyte elution as a function of time was examined by carrying out a paper spray analysis of dried plasma spots for 5 min by continually replenishing the spray solvent.

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“…Exploratory analysis of VOCs emitted by fungi typically involves a headspace analysis of in vitro cultures. VOCs-based biotyping has been used to identify species of Candida [93,94,98], Penicillium [123], Fusarium [124] and Rhizoctonia [125]. This type of biotyping can employ headspace absorptive extraction techniques associated with gas chromatography-time flight mass spectrometry (GC-TOF-MS) or liquid-liquid extraction methods associated with gas chromatography-mass spectrometry electron ionization (GC-MS-EI).…”

Section: Biotyping: Microbial Classification and Identificationmentioning

confidence: 99%

Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk

Calla-Quispe

Fuentes-Rivera

Ramírez

et al. 2020

Life

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Fungi are a highly diverse group of heterotrophic organisms that play an important role in diverse ecological interactions, many of which are chemically mediated. Fungi have a very versatile metabolism, which allows them to synthesize a large number of still little-known chemical compounds, such as soluble compounds that are secreted into the medium and volatile compounds that are chemical mediators over short and long distances. Mass spectrometry (MS) is currently playing a dominant role in mycological studies, mainly due to its inherent sensitivity and rapid identification capabilities of different metabolites. Furthermore, MS has also been used as a reliable and accurate tool for fungi identification (i.e., biotyping). Here, we introduce the readers about fungal specialized metabolites, their role in ecological interactions and provide an overview on the MS-based techniques used in fungal studies. We particularly present the importance of sampling techniques, strategies to reduce false-positive identification and new MS-based analytical strategies that can be used in mycological studies, further expanding the use of MS in broader applications. Therefore, we foresee a bright future for mass spectrometry-based research in the field of mycology.

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Discrimination of Candida species by paper spray mass spectrometry

Cited by 26 publications

References 70 publications

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk

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