Discrimination of Campylobacter jejuni isolates by fla gene sequencing

Meinersmann, Richard J.; Helsel, Leta O.; Fields, Patricia I.; Hiett, Kelli L.

doi:10.1128/jcm.35.11.2810-2814.1997

Cited by 201 publications

(95 citation statements)

References 12 publications

(24 reference statements)

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“…Carriage of multiple STs was lower among farmed ducks (1-3 STs per bird, average D = 0.19, range 0-0.56) compared with wild ducks (1-5 STs per bird, average D = 0.42, range 0-0.86). Only one ST, the C. jejuni ST-45, with fine type flaA SVR allele 2, peptide 27, was shared between both host types, accounting for 0.9% of wild duck isolates and 5% of farmed duck isolates (Meinersmann et al, 1997;Dingle et al, 2002). This genotype is thought to show adaption for environmental survival and is frequently isolated from water (Kwan et al, 2008;Sopwith et al, 2008;Carter et al, 2009).…”

Section: Campylobacter Species and Sequence Type Diversitymentioning

confidence: 99%

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Colles

Ali

Sheppard

et al. 2011

Environ Microbiol Rep

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Identifying the Campylobacter genotypes that colonize farmed and wild ducks will help to assess the proportion of human disease that is potentially attributable to the consumption of duck meat and environmental exposure to duck faeces. Comparison of temporally and geographically matched farmed and wild ducks showed that they had different Campylobacter populations in terms of: (i) prevalence, (ii) Campylobacter species and (iii) diversity of genotypes. Furthermore, 92.4% of Campylobacter isolates from farmed ducks were sequence types (STs) commonly associated with human disease, in contrast to just one isolate from the wild ducks. Only one ST, ST-45, was shared between the two sources, accounting for 0.9% of wild duck isolates and 5% of farmed duck isolates. These results indicate that domestic ‘niche’ as well as host type may affect the distribution of Campylobacter, and that husbandry practises associated with intensive agriculture may be involved in generating a reservoir of human disease associated lineages.

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Section: Campylobacter Species and Sequence Type Diversitymentioning

confidence: 99%

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Colles

Ali

Sheppard

et al. 2011

Environ Microbiol Rep

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“…To confirm flock and environmental matches, flagellin gene typing of selected strains was undertaken by sequencing of the PCR product of the flaA short variable region (SVR) with the primers FLA242FU and FLA625RU (Meinersmann et al 1997). A 321 bp sequence containing the flaA SVR nucleotide sequence was then compared with the database at http://pubmlst.org/campylobacter/ flaA/ (Dingle et al 2005).…”

Section: Molecular Typingmentioning

confidence: 99%

Potential sources of Campylobacter infection on chicken farms: contamination and control of broiler-harvesting equipment, vehicles and personnel

Ridley

Morris

Gittins

et al. 2011

Journal of Applied Microbiology

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Aims: To test the efficacy of enhanced biosecurity measures on poultry farms for reducing environmental contamination with Campylobacter during partial depopulation of broiler flocks prior to normal slaughter age. The study has also evaluated the risk of infection from live‐bird transport crates that are routinely cleaned at the slaughterhouse, but may remain contaminated. Methods and Results: On‐farm sampling and Campylobacter isolation was undertaken to compare the prevalence of contamination on vehicles, equipment and catching personnel during farm visits that took place under normal or enhanced biosecurity. Campylobacters were found in almost all types of sample examined and enhanced biosecurity reduced the prevalence. However, the additional measures failed to prevent colonisation of the flocks. For transport crates, challenge trials involved exposure of broilers to commercially cleaned crates and genotyping of any campylobacters isolated. The birds were rapidly colonised with the same genotypes as those isolated from the cleaned crates. Conclusions: The enhanced biosecurity measures were insufficient to prevent flock colonisation, and the problem was exacerbated by inadequate cleaning of transport crates at the slaughterhouse. Significance and Impact of the Study: Current commercial practices in the United Kingdom facilitate the spread of campylobacters among broiler chicken flocks. Prevention of flock infection appears to require more stringent biosecurity than that studied here.

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“…FlaA short variable region (SVR) sequencing was performed on the same subset of 52 isolates according to the standardized Oxford protocol (Clark et al 2005). The extracted DNA (same as for MLST) was used as a template in a PCR, using primers described previously (Meinersmann et al 1997). Amplification products were verified by gel electrophoresis.…”

Section: Fla-svr Sequencingmentioning

confidence: 99%

Comparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing

Magnússon

Gudmundsdóttir

Reynisson

et al. 2011

Journal of Applied Microbiology

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Aims: Campylobacter jejuni isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans. Methods and Results: A collection of 584 Campylobacter isolates were collected from clinical cases, food, animals and environment in Iceland in 1999-2002, during a period of national Campylobacter epidemic in Iceland. All isolates were characterized by pulse field gel electrophoresis (PFGE), and selected subset of 52 isolates representing the diversity of the identified PFGE types was further genotyped using multilocus sequence typing (MLST) and fla-SVR sequencing to gain better insight into the population structure. Conclusions:The results show a substantial diversity within the Icelandic Campylobacter population. Majority of the human Campylobacter infections originated from domestic chicken and cattle isolates. MLST showed the isolates to be distributed among previously reported and common sequence type complexes in the MLST database. Significance and Impact of the Study: The genotyping of Campylobacter from various sources has not previously been reported from Iceland, and the results of the study gave a valuable insight into the population structure of Camp. jejuni in Iceland, source distribution and transmission routes to humans. The geographical isolation of Iceland in the north Atlantic provides new information on Campylobacter population dynamics on a global scale.

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Discrimination of Campylobacter jejuni isolates by fla gene sequencing

Cited by 201 publications

References 12 publications

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Potential sources of Campylobacter infection on chicken farms: contamination and control of broiler-harvesting equipment, vehicles and personnel

Comparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing

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