Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant  Assay and Chemical Profiles Combined with  Multivariate Statistical Analysis

Chan, Chi-On; Lau, Chi Keung Marco; Ng, Yam-Fung; Xu, Lijia; Chen, Sibao; Chan, Shun‐Wan; Mok, Daniel K. W.

doi:10.3390/antiox4020359

Cited by 14 publications

(12 citation statements)

References 16 publications

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“…19 . Previous studies using DPPH assay and our results tested by DPPH and FRAP indicated that A. venetum has high antioxidant capacity 28,29 , which were similar with that of common black tea. Li application did not decrease the antioxidant capacity in A. venetum .…”

Section: Discussionsupporting

confidence: 77%

Lithium biofortification of medicinal tea Apocynum venetum

Jiang

Wang

Tanveer

et al. 2019

Sci Rep

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Lithium (Li) could be much safer and successful approach to supply Li via Li-fortified food products. This study is highlighting the potential scope of Li supply via Li-biofortification of Luobuma tea (made from Apocynum venetum leaves), which is a very popular beverage in Asia with several medical properties. We explored the possibility of A. venetum as Li-enriched tea and investigated plant growth, Li accumulation, total flavonoids (TFs), rutin and hyperoside concentrations, and the antioxidant capacity of A. venetum . With the increase of additional Li, Li concentration in roots, stems and leaves increased gradually. Compared with the control treatment, 10–15 mg kg −1 Li addition stimulated the growth of A. venetum and 25 mg kg −1 Li addition significantly increased the Li concentration in leaves by 80 mg kg −1 . Li application did not decrease TFs, rutin, hyperoside and antioxidant capacity of this medicinal herb. A daily consumption of 10 g Li-biofortified A. venetum leaves (cultivated with 25 mg kg −1 LiCl) can give 592 μg Li intake and would constitute 59% of the provisional recommended dietary daily intake of Li. Our results showed that Li-biofortified A. venetum leaves can be used as Li-fortified tea to enhance Li supply and to improve human health when it was used as daily drink.

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Section: Discussionsupporting

confidence: 77%

Lithium biofortification of medicinal tea Apocynum venetum

Jiang

Wang

Tanveer

et al. 2019

Sci Rep

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“…The results show the amount of flavonoids compounds selected in the two species to have varied tremendously in accordance with the LC/MC profile ( Table 5). The highest level of isoquercitrin (5.254 mg/g of DW, dry weight) was obtained in A. venetum, which was significantly higher than that obtained in A. hendersonii (1.718 mg/g of DW), suggesting that, in addition to hyperoside [7], isoquercitrin could also be a good chemical marker to differentiate A. venetum and A. hendersonii. The amount of total anthocyanins was determined using the previously described pH differential method.…”

Section: Analysis Of Flavonoids Biosynthesis-related Transcript Levelmentioning

confidence: 74%

“…A. venetum, in addition to serving as source of bast fiber, is very well known for possessing broad medicinal uses as anti-platelets [4], anti-depressive [5], anti-hypertensive [6], anti-oxidant [7], anti-aging [8], anxiolytic activity [1], stimulant as tea [9] etc. Meanwhile, A. hindersonii is morphologically similar to A. venetum but with a major visible distinction being in the color of stem and leaf, and the shape of their flower, owing likely to their local names "White Hemp" and "Red Hemp", respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Applications of A. hendersonii are usually limited to fibers for spinning and paper making, rather than medicinal purpose [8], due to the relative low total flavonoid content. Chan et al tentatively identified ten kinds of flavonoid components by ultra-visible and mass spectra, and hyperoside was identified as a critical parameter or a suitable chemical marker for discrimination of two species [7]. However, as A. venetum and A. hendersonii are distributed widely from the northwest to the coast of the Yellow Sea in China, the tremendous differences of the natural conditions may lead to the great differences in phenotype, structure, and chemical compositions.…”

Section: Introductionmentioning

confidence: 99%

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Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways

Gao

Chen

et al. 2019

Metabolites

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Apocynum plants, especially A. venetum and A. hendersonii, are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb (A. venetum) and 233.74 Mb (A. hendersonii) and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in A. venetum were found to be much higher than in A. hendersonii, and such was thought to be the reason for the morphological difference in color of A. venetum and A. hendersonii. This study provides valuable genomic and metabolome information for understanding of A. venetum and A. hendersonii, and lays a foundation for elucidating Apocynum genus plant flavonoid biosynthesis.

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“…Previous studies have shown that A. venetum has a higher flavonoid content than its related species Apocynum. hendersonii , and some flavonoid components such as hyperoside have been identified as suitable chemical markers for the discrimination of the two species [ 34 ]. In a recent metabolome and transcriptome analyses of Apocynum, Gao et al [ 21 ] found that the flavonoid biosynthetic pathway is responsible for a considerable proportion of the diversity between A. venetum and A. hendersonii , and identified anthocyanin as the key component that determines the phenotypic diversity of the stem and leaf color of A. venetum and A. hendersonii .…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome assembly and population genetic analyses of an important coastal shrub, Apocynum venetum L

Jia

2020

BMC Plant Biol

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Background: Apocynum venetum L. is an important medicinal plant that is mainly distributed in the coastal areas and northwest of China. In addition to its high medical and economic value, its adaptation to saline-alkali and coastal saline lands makes A. venetum an ideal candidate for use in vegetation restoration. To date, the study of A. venetum has been limited in the northwest region of China, little attention has been paid to the genetic diversity and population structure of A. venetum populations in the coastal region. Here, we performed transcriptome sequencing of total RNA from A. venetum leaves and developed efficient expressed sequence tag-simple sequence repeat (EST-SSR) markers for analyzing the genetic diversity and population structure of A. venetum in the coastal region. Results: A total of 86,890 unigenes were generated after de novo assembly, and 68,751 of which were successfully annotated by searching against seven protein databases. Furthermore, 14,072 EST-SSR loci were detected and 10, 243 primer pairs were successfully designed from these loci. One hundred primer pairs were randomly selected and synthesized, twelve primer pairs were identified as highly polymorphic and further used for population genetic analysis. Population genetic analyses showed that A. venetum exhibited low level of genetic diversity (mean alleles per locus, N A = 3.3; mean expected heterozygosity, H E = 0.342) and moderate level of genetic differentiation among the populations (genetic differentiation index, F ST = 0.032-0.220) in the coastal region. Although the contemporary (mean m c = 0.056) and historical (mean m h = 0.106) migration rates among the six A. venetum populations were moderate, a decreasing trend over the last few generations was detected. Bayesian structure analysis clustered six populations into two major groups, and genetic bottlenecks were found to have occurred in two populations (QG, BH). Conclusions: Using novel EST-SSR markers, we evaluated the genetic variation of A. venetum in the coastal region and determined conservation priorities based on these findings. The large dataset of unigenes and SSRs identified in our study, combining samples from a broader range, will support further research on the conservation and evolution of this important coastal plant and its related species.

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Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant Assay and Chemical Profiles Combined with Multivariate Statistical Analysis

Cited by 14 publications

References 16 publications

Lithium biofortification of medicinal tea Apocynum venetum

Lithium biofortification of medicinal tea Apocynum venetum

Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways

De novo transcriptome assembly and population genetic analyses of an important coastal shrub, Apocynum venetum L

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