Discrimination and goodness of fit of multiresponse mechanistic models

Stewart, W. D. P.; Shon, Youkang; Box, George E. P.

doi:10.1002/aic.690440618

Cited by 89 publications

(66 citation statements)

References 40 publications

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“…The effects of varying both time and concentration of the two key process steps, fixation by formaldehyde and lyse/permeabilization by Triton X-100, on light scatter and ZAP-70 expression were examined by a design of experiments (DOE) using either a Box-Behnken (27) or a central composite model (28) and implemented using Design-Expert TM 5 software (Stat-Ease, Inc., Minneapolis, MN). Formaldehyde concentrations from 3% to 5% and Triton X-100 concentrations from to 0.1% to 0.4% were examined in relation to incubation times of 5-15 min or 10-20 min, respectively.…”

Section: Whole Blood Optimization For Zap-70 Stainingmentioning

confidence: 99%

An optimized whole blood method for flow cytometric measurement of ZAP‐70 protein expression in chronic lymphocytic leukemia

Shankey

Forman

Scibelli

et al. 2006

Cytometry Part B Clinical

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Results: Using the optimized fixation and permeabilization method, improvement in assay performance (signal-to-noise, S/N) was seen in most of the antibodies tested. The custom SBZAP conjugate gave the best S/N when used in conjunction with this optimized fixation /permeabilization method. In conjunction with carefully standardized instrument set-up protocols, we obtained both intra-and interlaboratory reproducibility in the analysis of ZAP-70 expression in whole blood samples from normal and CLL patients.Conclusions: The development of a sensitive, specific and highly reproducible ZAP-70 assay represents only the first essential step for any clinical assay. The universal implementation of a validated data analysis method and the establishment of methodology-based cutoff points for clinical outcomes must next be established before ZAP-70 protein analysis can be routinely implemented in the clinical laboratory.

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Section: Whole Blood Optimization For Zap-70 Stainingmentioning

confidence: 99%

An optimized whole blood method for flow cytometric measurement of ZAP‐70 protein expression in chronic lymphocytic leukemia

Shankey

Forman

Scibelli

et al. 2006

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

“…The number of data points is given by n. The observations are left censored as indicated by c i , where c i is 0 if D i O 10 2 and 1 otherwise. The standard deviation of the measurement error, s, was unfortunately not available, and so was estimated by considering it as a parameter that must be fitted (Stewart et al 1998).…”

Section: Model Fitting Methodsmentioning

confidence: 99%

Effect of the initial dose of foot-and-mouth disease virus on the early viral dynamics within pigs

Howey

Quan

Savill

et al. 2008

J. R. Soc. Interface.

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This paper investigates the early viral dynamics of foot-and-mouth disease (FMD) within infected pigs. Using an existing within-host model, we investigate whether individual variation can be explained by the effect of the initial dose of FMD virus. To do this, we consider the experimental data on the concentration of FMD virus genomes in the blood (viral load). In this experiment, 12 pigs were inoculated with one of three different doses of FMD virus: low; medium; or high. Measurements of the viral load were recorded over a time course of approximately 11 days for every 8 hours. The model is a set of deterministic differential equations with the following variables: viral load; virus in the interstitial space; and the proportion of epithelial cells available for infection, infected and uninfected. The model was fitted to the data for each animal individually and also simultaneously over all animals varying only the initial dose. We show that the general trend in the data can be explained by varying only the initial dose. The higher the initial dose the earlier the development of a detectable viral load.

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“…ATHENA visual workbench software (Stewart et al, 1996) is a fully integrated environment for process modeling and nonlinear parameter estimation, is fully automated, and requires a Fortran compiler to operate. The software includes the solver GREGPLUS for the nonlinear parameter estimation with weighted least-square or/and Bayesian estimators (Bard, 1974;Stewart et al, 1992Stewart et al, , 1996Stewart et al, , 1998. GREGPLUS computes modal and interval estimates of the parameters in a user-provided Fortran subroutine MODEL, for single-or multiresponse observations.…”

Section: Parameter Estimationmentioning

confidence: 99%