Discrimination and characterization of Panax polysaccharides by 2D COS-IR spectroscopy with chemometrics

Lü, Yan-Li; Liang, Jun; Zhou, Fang-Yu; Kuang, Hai‐Xue; Xia, Yueyuan

doi:10.1016/j.ijbiomac.2021.04.124

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…The dry plant samples (each, 3 g), P. quinquefolium roots and A. bidentata roots, were washed thoroughly with water to remove any impurities, then dried, and ground into powders and the powders were sieved through a 40‐mesh drug sieve. The samples were soaked in distilled water for 12 h, extracted by ultrasound for 45 min, and extracted twice 25 . The extracted liquid was combined and concentrated to 5 mL, then 15 mL absolute ethanol was added and precipitated for 12 h. After centrifugation (2980 × g , 10 min), the precipitate was collected and redissolved with distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were soaked in distilled water for 12 h, extracted by ultrasound for 45 min, and extracted twice. 25 The extracted liquid was combined and concentrated to 5 mL, then 15 mL absolute ethanol was added and precipitated for 12 h. After centrifugation (2980 Â g, 10 min), the precipitate was collected and redissolved with distilled water. The Sevage reagent (n-butanol-chloroform = 1:3) was added to remove proteins, and the ratio of the Sevage reagent to the liquid was 4:1.…”

Section: Extractions Of Polysaccharidesmentioning

confidence: 99%

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Complete composition analysis of polysaccharides based on HPAEC‐PAD coupled with quantitative analysis of multi‐components by single marker

Yan,

Liu,

Liang

et al. 2023

Phytochemical Analysis

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IntroductionMonosaccharide compositions analysis (MCA) is indispensable for structural characterisations and structure–activity relationships of plant polysaccharides.ObjectivesTo develop a concise and direct MCA method, we established a quantitative analysis of the multi‐monosaccharaides by single marker (QAMS) by high‐performance anion‐exchange chromatography with pulsed‐amperometric detection (HPAEC‐PAD) method.MethodologyA stable and reproducible HPAEC‐PAD method for simultaneous determination of aldoses, ketoses and uronic acids (i.e., l‐arabinose, d‐xylose, d‐ribose, l‐rhamnose, d‐fucose, d‐mannose, d‐glucose, d‐galactose, d‐fructose, d‐glucuronic acid and d‐galacturonic acid) was established by systematic optimisation of stationary phases, column temperatures and elution programmes. On this basis, the QAMS method was proposed through comprehensive investigations of relative correction factor (RCF) variations under different influencing factors, for example, sample concentrations, flow rates, and column temperatures.ResultsUsing rhamnose as an internal reference standard, the contents of the other monosaccharide components in polysaccharides from Panax quinquefolium L. and Achyranthes bidentata Bl. samples were simultaneously determined by QAMS, and there was no significant difference between the results from the QAMS and external standard method (t test, P > 0.520). In addition, a MCA fingerprinting of 30 batches of P. quinquefolium polysaccharide was established by HPAEC‐PAD, and six common peaks were assigned and determined.ConclusionsThe established HPAEC‐PAD‐QAMS method was successfully applied to the MCA of polysaccharides from P. quinquefolium and A. bidentata after optimisation of hydrolysis conditions. HPAEC‐PAD‐QAMS was proposed and established for MCA of plant polysaccharides for the first time.

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Section: Methodsmentioning

confidence: 99%

Section: Extractions Of Polysaccharidesmentioning

confidence: 99%

Complete composition analysis of polysaccharides based on HPAEC‐PAD coupled with quantitative analysis of multi‐components by single marker

Yan,

Liu,

Liang

et al. 2023

Phytochemical Analysis

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“…The monosaccharide composition was analyzed by HPLC, and it was found that there was a large difference in the monosaccharide composition of the two components. A study proposed a new two-dimensional correlation infrared spectroscopy (2DCOS-IR) method for the identification of ginseng polysaccharides [28]. 2D-sATR-FTIR has the advantages of high throughput and high efficiency in polysaccharide quality evaluation of ginseng polysaccharides.…”

Section: Ginsengmentioning

confidence: 99%

Research Progress on Extraction, Isolation, Structural Analysis and Biological Activity of Polysaccharides from Panax Genus

et al. 2023

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The panax genus is a widely used medicinal plant with good biological activity. As one of the main active components of the Panax genus, polysaccharides have various pharmacological effects. This review summarizes the latest research reports on ginseng, American ginseng, and Panax notoginseng polysaccharides and compares the differences in extraction, isolation and purification, structural characteristics, and biological activities. The current research mainly focuses on ginseng polysaccharides, and the process of extraction, isolation, and structure analysis of each polysaccharide is roughly the same. Modern pharmacological studies have shown that these polysaccharides have antioxidants, antitumor, immunomodulatory, antidiabetic, intestinal protection, skin repair, and other biological activities. This review provides new insights into the differences between the three kinds of ginseng polysaccharides which will help to further study the medicinal value of ginseng in traditional Chinese medicine.

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“…As recorded in the research, the constructed 2D COS-IR method combined with chemometric analysis has achieved the quick and effective identification and discrimination of polysaccharides in Chinese medicine. It provides an important methodological reference for us to evaluate the quality of traditional Chinese medicine polysaccharide [141]. In terms of the analysis strategy, the following studies have made outstanding contributions to the quality control of traditional Chinese medicine polysaccharides.…”

Section: Quality Controlmentioning

confidence: 99%

A Systematic Review on the Research Progress on Polysaccharides from Fungal Traditional Chinese Medicine

Bai,

Su,

Zhang

et al. 2023

Molecules

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Traditional Chinese medicine (TCM) is a class of natural drugs with multiple components and significant therapeutic effects through multiple targets. It also originates from a wide range of sources containing plants, animals and minerals, and among them, plant-based Chinese medicine also includes fungi. Fungal traditional Chinese medicine is a medicinal resource with a long history and widespread application in China. Accumulating evidence confirms that polysaccharide is the main pharmacodynamic material on which fungal TCM is based. The purpose of the current systematic review is to summarize the extraction, isolation, structural identification, biological functions, quality control and medicinal and edible applications of polysaccharides from fungal TCM in the past three years. This paper will supplement and deepen the understanding and application of polysaccharides from fungal TCM, and propose some valuable insights for further research and development of drugs and functional foods.

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Discrimination and characterization of Panax polysaccharides by 2D COS-IR spectroscopy with chemometrics

Cited by 18 publications

References 29 publications

Complete composition analysis of polysaccharides based on HPAEC‐PAD coupled with quantitative analysis of multi‐components by single marker

Complete composition analysis of polysaccharides based on HPAEC‐PAD coupled with quantitative analysis of multi‐components by single marker

Research Progress on Extraction, Isolation, Structural Analysis and Biological Activity of Polysaccharides from Panax Genus

A Systematic Review on the Research Progress on Polysaccharides from Fungal Traditional Chinese Medicine

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