Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization

Humphreys, M. W.; Thomas, Hugh; Morgan, W. G.; Meredith, M. R.; Harper, John A.; Zwierzykowski, Z.; Ghesquière, Marc

doi:10.1038/hdy.1995.120

Cited by 111 publications

(73 citation statements)

References 12 publications

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“…pratensis has been proposed as one of the progenitors of the hexaploid genome (Humphreys et al 1995). One possible reason why the S Fa against Lolium (0.68 and 0.59 against L. perenne and L. multiflorum, respectively) is less than the S Fp with regard to Lolium (0.84 and 0.72) is the possible divergence of the P genome during evolution in the hexaploid from its original diploid progenitor (Chen et al 1998 (Fig.…”

Section: Species Specificities Of the Markers Within Combinations Of mentioning

confidence: 99%

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

et al. 2009

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DNA markers able to distinguish species or genera with high specificity are valuable in the identification of introgressed regions in interspecific or intergeneric hybrids.Intergeneric hybridization between the genera of Lolium and Festuca, leading to the reciprocal introgression of chromosomal segments, can produce novel forage grasses with unique combinations of characteristics. To characterize Lolium/Festuca introgressions, novel PCR-based expression sequence tag (EST) markers were developed. These markers were designed around intronic regions which show higher polymorphism than exonic regions. Intronic regions of the grass genes were predicted from the sequenced rice genome. Two hundred and nine primer sets were designed from Lolium/Festuca ESTs that showed high similarity to unique rice genes dispersed uniformly throughout the rice genome. We selected 61 of these primer sets as insertion-deletion (indel)-type markers and 82 primer sets as cleaved amplified polymorphic sequence (CAPS) markers to distinguish between Lolium perenne and Festuca pratensis. Specificity of these markers to each species was evaluated by the genotyping of four cultivars and accessions (32 individuals) of L. perenne and F. pratensis respectively. Evaluation using specificity indices proposed in this study suggested that many indel-type markers had high species specificity to L. perenne and F. pratensis, including 15 markers completely specific to both species. Forty-nine of the CAPS markers completely distinguish between the two species at bulk level.Chromosome mapping of these markers using a Lolium/Festuca substitution line 3 revealed syntenic relationships between Lolium/Festuca and rice largely consistent with previous reports. This intron-based marker system that shows a high level of polymorphisms between species in combination with high species specificity will consequently be a valuable tool in Festulolium breeding. Keywordsintergeneric (interspecific) hybrid -intron polymorphismsinsertion-deletion (indel) -specificity to species -rice genome -comparative genomics

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Section: Species Specificities Of the Markers Within Combinations Of mentioning

confidence: 99%

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

et al. 2009

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“…A good example was the determination of the ancestry of F. arundinacea Schreb. (Humphreys et al, 1995 In addition to the hybridization that has occurred naturally between species within Festuca subg. Schedonorus, there are equivalent occurrences between these Festuca species and representatives of genus Lolium.…”

Section: Genomic In Situ Hybridization (Gish) and Fluorescence In Sitmentioning

confidence: 99%

“…Incidents of heterosis abound within the species of the Lolium-Festuca complex capable of providing adaptive traits that otherwise are unavailable to their parental genotypes. The hexaploid species F. arundinacea is a good example (Humphreys et al, 1995).…”

Section: Genomic In Situ Hybridization (Gish) and Fluorescence In Sitmentioning

confidence: 99%

“…arundinacea Shreb. is an allohexaploid species developed from hybridization of F. pratensis and tetraploid F. glaucescens (Humphreys et al, 1995). Consequently, labeled gDNAs of F. glaucescens and F. pratensis were selected as probes for GISH and applied to denatured chromosomes of the pentaploid plants.…”

Section: Genomic In Situ Hybridization (Gish) and Fluorescence In Sitmentioning

confidence: 99%

“…(2n = 4x = 28) that gave rise to the now widely dispersed hexaploid tall fescue (Festuca arundinacea Schreb.) (2n = 6x = 42), used widely in grassland agriculture and in particular where abiotic stresses are too severe for less well adapted Lolium varieties (Humphreys et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An Increasing Need for Productive and Stress Resilient Festulolium Amphiploids: What Can Be Learnt from the Stable Genomic Composition of Festuca pratensis subsp. apennina (De Not.) Hegi?

Kopecký

Harper

Bartoš

et al. 2016

Front. Environ. Sci.

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Designing agricultural grasses to help mitigate proteolysis during ensiling to optimize protein feed provisions for livestock

Muhandiram

Humphreys

Fychan

et al. 2023

Food and Energy Security

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The efficient preservation of protein in silage for livestock feed is dependent on the rate and extent of proteolysis. Previous research on fresh forage indicated enhanced protein stability in certain Festulolium (ryegrass × fescue hybrids) cultivars compared to ryegrass. This is the first report of an experiment to test the hypothesis that a Lolium perenne × Festuca arundinacea var glaucescens cultivar had reduced proteolysis compared to perennial ryegrass (L. perenne) during the ensiling process. Forages were harvested in May (Cut 2) and August (Cut 4), wilted for 24 h and ensiled in laboratory-scale silos. Silage was destructively sampled at 0 h, 9 h, 24 h, 48 h, 72 h, 14 days and 90 days post-ensiling, and dry matter (DM), pH and chemical composition were determined. At Cut 2, there was no difference in crude protein between treatments but ryegrass had higher soluble nitrogen (SN) (P < 0.001) and grass × time interactions (p = 0.03) indicated higher rates of proteolysis. By Cut 4, Festulolium had (5.5% units) higher CP than ryegrass (p < 0.001) but SN did not differ. Ammonia-N did not differ between silages in either cut. DM differences (11.8% units) between treatments in Cut 4 (v.2.2% in Cut 2) may have masked effects on proteolysis, highlighting the importance of management on silage quality. This was despite higher WSC in ryegrass in both cuts (p < 0.001), with grass × time interactions (Cut 2; p = 0.03) showing slower WSC decline in ryegrass in Cut 4 (p < 0.001). Silage pH values did not differ between grasses in either cut, but grass × time interactions (p < 0.001) showed a slower decline in both ryegrass cuts, resulting in higher (p < 0.05) pH at 24 h and 72 h for Cuts 2 and 4, respectively.Overall, the hypothesis for an enhanced protein stability in Festulolium when ensiled as ruminant feed was evidenced by lower SN but not ammonia-N in an earlycut silage with a comparable DM to ryegrass.

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Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization

Cited by 111 publications

References 12 publications

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

An Increasing Need for Productive and Stress Resilient Festulolium Amphiploids: What Can Be Learnt from the Stable Genomic Composition of Festuca pratensis subsp. apennina (De Not.) Hegi?

Designing agricultural grasses to help mitigate proteolysis during ensiling to optimize protein feed provisions for livestock

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