Discriminating activated sludge flocs from biofilm microbial communities in a novel pilot-scale reciprocation MBR using high-throughput 16S rRNA gene sequencing

Sotto, Ryan De; Ho, Jaeho; Lee, Woonyoung; Bae, Sung Woo

doi:10.1016/j.jenvman.2018.03.081

Cited by 25 publications

(3 citation statements)

References 35 publications

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“…As economies develop, the environment is being irresistibly degraded, leading to more stringent sewage discharge standards at wastewater treatment plants (WWTPs) to prevent environmental pollution [1][2][3]. Membrane bioreactors (MBRs) have emerged as a superior technology to the conventional activated sludge (CAS) process due to their high pollutant removal rate, compact footprint, high effluent quality, flexible operation, minimal sludge formation, and high biomass concentration [4][5][6][7]. In fact, MBRs are capable of removing 30-67% total nitrogen (TN) and 12-59% total phosphorus (TP) from domestic wastewater [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Municipal Sewage Treatment Technology: A2/O-VMBR Integrated Technology for Municipal Treatment and Improved Pollutant Removal

Han

Lyu

et al. 2023

Water

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To modernize wastewater treatment plants, a pilot-scale anaerobic/anoxic/oxic-vibrating membrane bioreactor (A2/O-VMBR) was developed and successfully operated. Despite a low C/N ratio, the A2/O-VMBR achieved removal rates of 61.10%, 93.77%, 72.86%, and 54.75% for COD, TN, TP, and NH3-N, respectively. The maximum and extremity transmembrane pressures were 45 kPa, and 80 kPa, respectively, with no sludge bulking observed. The VMBR saved over 96–98% of energy compared to traditional MBR plants, making it a better option for municipal wastewater treatment. High-throughput sequencing analysis revealed identical bacterial population structures in samples obtained from the treatment units, with genera having nitrifying, denitrifying, hydrolyzing, and glycogen-accumulating activities, which allowed for nitrogen removal. The key functional microorganisms responsible for nitrification–denitrification were species belonging to the genera FCPU426, Fusobacteria, Planctomycetes, Verrucomicrobia, and Epsilonbacteraeota. The integrated experimental system produced favorable results in improving wastewater quality, highlighting the usability of the A2/O-VMBR technology. Therefore, this technique holds potential for further investigation into the context of wastewater treatment and recovery.

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Section: Introductionmentioning

confidence: 99%

Municipal Sewage Treatment Technology: A2/O-VMBR Integrated Technology for Municipal Treatment and Improved Pollutant Removal

Han

Lyu

et al. 2023

Water

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“…Backwashing as an effective membrane control method has been reported to successfully remove fouling deposits and is particularly convenient as a part of a deadend hollow fiber filtration cycle (Cui et al, 2017;Cui et al, 2018). Previous studies focused on the intensity, optimal backwash frequency, duration and air sparging to improve backwash efficiency (De Sotto et al, 2018;Shekhar et al, 2017;Yigit et al, 2009). The aim of a series of tests in this study is to investigate the effect of dynamic change of membrane fouling on effective backwashing length in MBR system with the evolution software of CFD.…”

Section: Introductionmentioning

confidence: 99%

Investigation of backwashing effectiveness in membrane bioreactor (MBR) based on different membrane fouling stages

Zhao

Wang

Zhang

et al. 2018

Bioresource Technology

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In this study the effect of different fouling stages of hollow fiber membranes on effective backwashing length in MBR has been investigated. Computational fluid dynamics (CFD) is imported to simulate backwashing process. A multi-physics coupling model for free porous media flow, convective mass transfer and diluted species transport was established. The laser bijection sensors (LBS) were imported to monitor the backwashing solution position inside fiber lumen. Simulation results indicated that membrane fouling degree could change the velocity of backwash solution inside fiber lumen and make a further effect on effective backwash length. The signal variations of LBS are in accordance with the simulation results. The backwashing process can only play an active role when the filtration pressure is below the critical TMP. It can be concluded that backwash duration in industrial applications need to be set based on changes in TMP.

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“…Introduction Nitrogen (N) removal in sewage is one of the main goals of biological sewage treatment. High N emissions can cause accelerated eutrophication in a receiving water body (De Sotto et al, 2018). The sequencing batch reactor (SBR) activated sludge process is commonly used in sewage treatment plants, offering advantages in terms of footprint, investment, energy consumption, as well as good removal of organic matter and phosphate (Maslon et al, 2019).…”

mentioning

confidence: 99%

Microbial composition and nitrogen removal pathways in a novel sequencing batch reactor integrated with semi-fixed biofilm carrier: Evidence from a pilot study for low- and high-strength sewage treatment

Cui¹

2021

Preprint

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The sequencing batch reactor (SBR) activated sludge process is a well-established technology for sewage treatment. One of the drawbacks of SBRs, however, is the failure to conform to the standard for total nitrogen (TN) discharge. By means of introducing four improvements, including semi-fixed biofilm carrier, sludge elevation mixing and change for the mode of influent and effluent, compliant standard for TN discharge was obtained in this novel SBR configuration during low- and high- -strength sewage load. To illustrate the microbial compositions and functions of the attached biofilm on semi-fixed carrier and the suspended aggregates, as well as the nitrogen removal pathway, high throughput 16S rRNA gene amplicon sequencing, PICRUSt2 algorithm and KEGG database were applied. The results revealed that: i) the microbial communities from suspended aggregates and biofilm samples were significantly different from each other; ii) during low-strength sewage loads, TN removal was mainly by nitrification-denitrification. The suspended aggregates was responsible for denitrification, while the biofilm was focused on ammonium oxidation; iii) during high-strength sewage loads, function of nitrate reductase from suspended aggregates was faded, and anammox and N assimilation by biofilm became dominant. Meanwhile, TN removal referring to the formation of L-glutamine via assimilation was the main pathway.

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Discriminating activated sludge flocs from biofilm microbial communities in a novel pilot-scale reciprocation MBR using high-throughput 16S rRNA gene sequencing

Cited by 25 publications

References 35 publications

Municipal Sewage Treatment Technology: A2/O-VMBR Integrated Technology for Municipal Treatment and Improved Pollutant Removal

Municipal Sewage Treatment Technology: A2/O-VMBR Integrated Technology for Municipal Treatment and Improved Pollutant Removal

Investigation of backwashing effectiveness in membrane bioreactor (MBR) based on different membrane fouling stages

Microbial composition and nitrogen removal pathways in a novel sequencing batch reactor integrated with semi-fixed biofilm carrier: Evidence from a pilot study for low- and high-strength sewage treatment

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