Discrepancy in transcriptomic profiling between CD34 + stem cells and primary bone marrow cells in myelodysplastic neoplasm

Ribeiro, Howard Lopes; Gonçalves, Paola Gyuliane; Moreno, Daniel Antunes; Goes, João Vitor Caetano; Oliveira, Roberta Taiane Germano de; Montefusco-Pereira, Carlos Victor; Komoto, Tatiana Takahasi; Pinheiro, Ronald Feitosa

doi:10.1016/j.leukres.2023.107071

Cited by 2 publications

(3 citation statements)

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Section: Resultsmentioning

confidence: 99%

“…The differential gene expression analysis for UBE2O , UBE2T , USP7 , and USP15 was based on microarray data from 1092 patients previously published by our research group [ 26 ]. We compared gene expression data from patients with MDS, obtained from primary mononuclear bone marrow (PMBM) cells or CD34+ isolated stem cells, to healthy controls [ 26 ]. The analysis of the mononuclear BM cells microarray identified that UBE2T (MDS mean: 7.021 versus Control mean: 5.625; p < 0.001), UBE2O (MDS mean: 8.693 versus Control mean: 7.461; p < 0.001), and USP7 (MDS mean: 12.245 versus Control mean: 11.620; p < 0.001) were all upregulated in PMBM cells of MDS patients compared with healthy controls ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…To predict the expression profile of the USP15 , USP7 , UBE2T , and UBE2O genes in MDS, we used gene expression data based on analyses of Microarray databases from the Gene Expression Omnibus (GEO) database ( ) previously and recently published by Ribeiro-Junior et al [ 26 ]. The authors performed a microarray re-analysis from primary mononucleated bone marrows cell (700 MDS patients and 47 healthy individuals) and CD34+ hematopoietic stem cell (392 MDS patients and 44 healthy individuals) samples from a total of 1092 MDS patients [ 26 ]. We then used R packages to compare the expression of the USP15 , USP7 , UBE2T , and UBE2O genes between the control and MDS groups using Student’s t test.…”

Section: Methodsmentioning

confidence: 99%

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USP15-USP7 Axis and UBE2T Differential Expression May Predict Pathogenesis and Poor Prognosis in De Novo Myelodysplastic Neoplasm

Carvalho

Komoto

Moreno

et al. 2023

IJMS

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The aim of this study was to evaluate the expression of USP7, USP15, UBE2O, and UBE2T genes in Myelodysplastic neoplasm (MDS) to identify possible targets of ubiquitination and deubiquitination in MDS pathobiology. To achieve this, eight datasets from the Gene Expression Omnibus (GEO) database were integrated, and the expression relationship of these genes was analyzed in 1092 MDS patients and healthy controls. Our results showed that UBE2O, UBE2T, and USP7 were upregulated in MDS patients compared with healthy individuals, but only in mononucleated cells collected from bone marrow samples (p < 0.001). In contrast, only the USP15 gene showed a downregulated expression compared with healthy individuals (p = 0.03). Additionally, the upregulation of UBE2T expression was identified in MDS patients with chromosomal abnormalities compared with patients with normal karyotypes (p = 0.0321), and the downregulation of UBE2T expression was associated with MDS hypoplastic patients (p = 0.033). Finally, the USP7 and USP15 genes were strongly correlated with MDS (r = 0.82; r2 = 0.67; p < 0.0001). These findings suggest that the differential expression of the USP15-USP7 axis and UBE2T may play an important role in controlling genomic instability and the chromosomal abnormalities that are a striking characteristic of MDS.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%