“…To capture cryoEM structures of various biotin-binding complexes, we performed streptavidin pull-down of endogenous complexes from L. tarentolae mitochondrial lysate fractionated by glycerol density gradient and determined their structures. One subset of these structures has a three-fold symmetric architecture reminiscent of carboxylases ( Huang et al, 2010 , Tong, 2013 , Huang et al, 2012 , Jurado et al, 2015 , Hu et al, 2023 ); by using the cryoID approach ( Ho et al, 2020 ), we confirmed its identity as propionyl-CoA carboxylase from L. tarentolae (LtPCC).…”