Discovery proteomics: application to HIV infection

Haarburger, David; Pillay, Tahir S

doi:10.1136/jcp.2009.073148

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…But it will not be easy to translate these snapshot datasets into a comprehensive mechanistic understanding of all interactions involved (Haarburger and Pillay, 2010; Zhang et al, 2010). As mentioned, another problem of proteomic studies is identification and quantification of proteins with lower abundancies.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of HIV–T cell interaction: an update

Kramer¹,

Moerland²,

Jeeninga³

et al. 2012

Front. Microbio.

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This mini-review summarizes techniques applied in, and results obtained with, proteomic studies of human immunodeficiency virus type 1 (HIV-1)–T cell interaction. Our group previously reported on the use of two-dimensional differential gel electrophoresis (2D-DIGE) coupled to matrix assisted laser-desorption time of flight peptide mass fingerprint analysis, to study T cell responses upon HIV-1 infection. Only one in three differentially expressed proteins could be identified using this experimental setup. Here we report on our latest efforts to test models generated by this data set and extend its analysis by using novel bioinformatic algorithms. The 2D-DIGE results are compared with other studies including a pilot study using one-dimensional peptide separation coupled to MSE, a novel mass spectrometric approach. It can be concluded that although the latter method detects fewer proteins, it is much faster and less labor intensive. Last but not least, recent developments and remaining challenges in the field of proteomic studies of HIV-1 infection and proteomics in general are discussed.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of HIV–T cell interaction: an update

Kramer¹,

Moerland²,

Jeeninga³

et al. 2012

Front. Microbio.

View full text Add to dashboard Cite

show abstract

“…Mass spectrometry proteomic techniques have been extensively used to understand the dynamics of host-virus interactions with respect to HIV in several in-vitro studies, and have been previously reviewed [22,23]. Perhaps more importantly, these MS proteomic technologies have the potential to increase our understanding of the interplay between HIV and host ex-vivo responses.…”

Section: Use Of Quantitative Mass Spectrometry Approaches In Hiv Infementioning

confidence: 99%