Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera

Uthailak, Naphatsamon; Adisakwattana, Poom; Thiangtrongjit, Tipparat; Limpanont, Yanin; Chusongsang, Phiraphol; Chusongsang, Yupa; Tanasarnprasert, Kanthi; Reamtong, Onrapak

doi:10.1371/journal.pone.0275992

Cited by 5 publications

(9 citation statements)

References 95 publications

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“…S. haematobium has been classified as a probable human carcinogen by the International Agency for Research on Cancer (IARC) [ 21 ]. S. intercalatum and S. mekongi are two species that are locally widespread, but to a lesser extent than the other species [ 22 ]. S. intercalatum is common in the Democratic Republic of Congo, while S. mekongi is common in certain areas of Cambodia and Laos [ 23 ].…”

Section: Epidemiologymentioning

confidence: 99%

Insights into the epidemiology, pathogenesis, and differential diagnosis of schistosomiasis

Ponzo,

Midiri,

Manno

et al. 2024

EuJMI

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Schistosomiasis is a neglected tropical disease that is prevalent in low- and middle-income countries. There are five human pathogenic species, of which Schistosoma haematobium, Schistosoma mansoni and Schistosoma japonicum are the most prevalent worldwide and cause the greatest burden of disease in terms of mortality and morbidity. In addition, hybrid schistosomes have been identified through molecular analysis. Human infection occurs when cercariae, the larval form of the parasite, penetrate the skin of people while bathing in contaminated waters such as lakes and rivers. Schistosomiasis can cause both urogenital and intestinal symptoms. Urogenital symptoms include haematuria, bladder fibrosis, kidney damage, and an increased risk of bladder cancer. Intestinal symptoms may include abdominal pain, sometimes accompanied by diarrhoea and blood in the stool. Schistosomiasis affects more than 250 million people and causes approximately 70 million Disability-Adjusted Life Years (DALYs), mainly in Africa, South America, and Asia. To control infection, it is essential to establish sensitive and specific diagnostic tests for epidemiological surveillance and morbidity reduction. This review provides an overview of schistosomiasis, with a focus on available diagnostic tools for Schistosoma spp. Current molecular detection methods and progress in the development of new diagnostics for schistosomiasis infection are also discussed.

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Section: Epidemiologymentioning

confidence: 99%

Insights into the epidemiology, pathogenesis, and differential diagnosis of schistosomiasis

Ponzo,

Midiri,

Manno

et al. 2024

EuJMI

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“…Nucleic acid such as DNA of schistosomes can be released to the host’s blood circulation upon entry and during the parasite’s development [ 7 , 71 ]. Hence, the nucleic acid detection is useful in diagnosing active schistosome infection [ 25 , 59 ]. [ 55 ] likewise demonstrated that schistosome DNA can be detected in experimentally infected mice before parasite eggs are demonstrated on the feces.…”

Section: Diagnosis Of S Mekongi Infectionmentioning

confidence: 99%

“…Affecting approximately 250 million people worldwide, schistosomiasis has persisted as a public health concern in selected regions throughout Africa, South America, and Asia [ 17 ]. There are three known species of schistosomes affecting the Asian region: S. japonicum, S. mekongi , and S. malayensis [ 17 , 40 , 59 ]. Among the three, S. mekongi will be the focus of this review.…”

Section: Introductionmentioning

confidence: 99%

“…Its endemic distribution is limited to the areas inhabited by Neotricula aperta the intermediate host snail, whose range is limited by specific ecological conditions [ 3 , 27 , 47 , 53 ]. Similar to its closely related species S. japonicum , S. mekongi may likewise infect a variety of domestic animals including pigs, water buffalo, and cattle [ 59 ].…”

Section: Introductionmentioning

confidence: 99%

“…To address the current difficulty faced using traditional stool microscopy, several studies have been done to detect S. mekongi infection. Modern molecular biological techniques based on parasite-derived biomarkers and nucleic acid as well as host-derived antibodies indicative of infection has shown promising results [ 9 , 25 , 31 , 49 , 59 , 65 ]. Here, we review the current molecular and serological methods in detecting S. mekongi infection and its potential contribution towards the successful elimination of schistosomiasis mekongi.…”

Section: Introductionmentioning

confidence: 99%

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Current advances in serological and molecular diagnosis of Schistosoma mekongi infection

Macalanda,

Wanlop,

Ona

et al. 2024

Trop Med Health

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Schistosomiasis, a neglected tropical disease, caused by blood flukes belonging to the genus Schistosoma; it persists as a public health problem in selected regions throughout Africa, South America, and Asia. Schistosoma mekongi, a zoonotic schistosome species endemic to the Mekong River in Laos and Cambodia, is one of the significant causes of human schistosomiasis along with S. japonicum, S. mansoni, S. haematobium and S. intercalatum. Since its discovery, S. mekongi infection has been highly prevalent in communities along the Mekong River. Although surveillance and control measures have shown success in recent years, more robust diagnostic tools are still needed to establish more efficient control and prevention strategies to achieve and sustain an elimination status. Diagnosis of S. mekongi infection still relies on copro-parasitological techniques, commonly made by Kato-Katz stool examination. Serological techniques such as enzyme-linked immunosorbent assay (ELISA) may also be applicable but in a limited setting. Targeted molecular and serological tools specific to the species, on the other hand, have been limited. This is due, in part, to the limited research and studies on the molecular biology of S. mekongi since genome information of this species has not yet been released. In this review, current advances, and gaps and limitations in the molecular and immunological diagnosis of S. mekongi are discussed.

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Chromosome-scale genome of the human blood fluke Schistosoma mekongi and its implications for public health

Zhou,

Xu,

et al. 2023

Infect Dis Poverty

Self Cite

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Background Schistosoma mekongi is a human blood fluke causing schistosomiasis that threatens approximately 1.5 million humans in the world. Nonetheless, the limited available S. mekongi genomic resources have hindered understanding of its biology and parasite-host interactions for disease management and pathogen control. The aim of our study was to integrate multiple technologies to construct a high-quality chromosome-level assembly of the S. mekongi genome. Methods The reference genome for S. mekongi was generated through integrating Illumina, PacBio sequencing, 10 × Genomics linked-read sequencing, and high-throughput chromosome conformation capture (Hi-C) methods. In this study, we conducted de novo assembly, alignment, and gene prediction to assemble and annotate the genome. Comparative genomics allowed us to compare genomes across different species, shedding light on conserved regions and evolutionary relationships. Additionally, our transcriptomic analysis focused on genes associated with parasite-snail interactions in S. mekongi infection. We employed gene ontology (GO) enrichment analysis for functional annotation of these genes. Results In the present study, the S. mekongi genome was both assembled into 8 pseudochromosomes with a length of 404 Mb, with contig N50 and scaffold N50 lengths of 1168 kb and 46,759 kb, respectively. We detected that 43% of the genome consists of repeat sequences and predicted 9103 protein-coding genes. We also focused on proteases, particularly leishmanolysin-like metalloproteases (M8), which are crucial in the invasion of hosts by 12 flatworm species. Through phylogenetic analysis, it was discovered that the M8 gene exhibits lineage-specific amplification among the genus Schistosoma. Lineage-specific expansion of M8 was observed in blood flukes. Additionally, the results of the RNA-seq revealed that a mass of genes related to metabolic and biosynthetic processes were up-regulated, which might be beneficial for cercaria production. Conclusions This study delivers a high-quality, chromosome-scale reference genome of S. mekongi, enhancing our understanding of the divergence and evolution of Schistosoma. The molecular research conducted here also plays a pivotal role in drug discovery and vaccine development. Furthermore, our work greatly advances the understanding of host-parasite interactions, providing crucial insights for schistosomiasis intervention strategies. Graphical Abstract

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Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera

Cited by 5 publications

References 95 publications

Insights into the epidemiology, pathogenesis, and differential diagnosis of schistosomiasis

Insights into the epidemiology, pathogenesis, and differential diagnosis of schistosomiasis

Current advances in serological and molecular diagnosis of Schistosoma mekongi infection

Chromosome-scale genome of the human blood fluke Schistosoma mekongi and its implications for public health

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