Discovery of putative novel viruses in the transcriptomes of endangered plant species native to India and China

Transcriptome Mining Expands Knowledge of RNA Viruses across the Plant Kingdom

“…The copyright holder for this preprint this version posted February 8, 2022. ; https://doi.org/10.1101/2022.02.07.479358 doi: bioRxiv preprint in gymnosperms (8)(9)(10)(11)(12). Outside of several viruses found in ferns, we know little of the diversity of viruses in the lycophytes, bryophytes and charophytes that together encompass ~27,000 species (13)(14)(15)(16)) (Figure 1).…”

A glimpse into the DNA virome of the unique “living fossil” Welwitschia mirabilis

“…In the recent years, the analysis of public data constitutes an emerging source of novel bona fide plant viruses, which allows the reliable identification of new viruses in hosts with no previous record of virus infections [10,11]. In general, plant viruses reported to date based in metagenomic data have been detected analyzing publically available plant transcriptome datasets, resulting mostly in the detection of RNA viruses which could have been inadvertently co-isolated with host RNAs during library preparation [10,[12][13][14][15]. WGS DNA datasets have received less attention in virus discovery studies, perhaps given its higher complexity in terms of database size and technical difficulties during processing.…”

Exploring the tymovirids landscape through metatranscriptomics data

“…In the last few years, the employment of HTS, allowed the identification and characterization of several novel viruses associated to the order Tymovirales , which may be asymptomatic [27–33]. Moreover, some tymovirids were also discovered mining publically available transcriptome datasets at the NCBI [34–40]. Thus, many tymovirales-like sequences could be hidden in publicly available plant transcriptome datasets.…”