Discovery of PTE-1, Tourist-like miniature inverted repeat transposable element (MITE), and its activation in transgenic Brassica rapa ssp. pekinensis plants

Identification and Characterization of PTE-2, a Stowaway-like MITE Activated in Transgenic Chinese Cabbage Lines

“…MITE information of Chinese cabbage from P-MITE was mapped on a pseudomolecule of ‘CT001’. It was discovered that 280,501 MITEs from the P-MITE database are distributed in the ‘CT001’ genome [ 17 ].…”

“…Although DNA transposons are not replicated through the transposition process, PTE-2 , a class II element, has multiple copies within the genome ( Figure 4 ). MITEs are the major type of TEs, comprising approximately 4.05% of the Chinese cabbage genome by having 6637 different elements and 280,501 copies [ 17 ].…”

“…To detect activated MITEs in transgenic Chinese cabbage lines, a three-point analysis was conducted in a previous study [ 17 ]. The resequencing data of ‘IGA’ transgenic lines were aligned and compared with MITE-mapped ‘CT001’ pseudomolecule.…”

“…If MITE is activated, the length of the amplicon should be shorter than that of the inactivated amplicon. Primer sequences were obtained from previous research: 5′-TAT ACA TGA CGA GTA TAC GAG GG-3′ as the forward primer and 5′-CCA CAA GTG ATC GTT GTC TAG-3′ as the reverse primer [ 17 ]. BioFACT™ 2X Taq PCR Pre-Mix (BIOFACT, Seoul, Korea) was used to carry out PCR amplification with 10 pmol of forward primer, 10 pmol of reverse primer, and 50–100 ng of gDNA template.…”

“…Both showed a higher transposition activation ratio in transgenic plants than in regenerated plants [ 15 , 16 ]. In Chinese cabbage (also called kimchi cabbage), a MITE named PTE-1 was found to be activated by the transformation procedure [ 17 ]. In rice, mPing, a MITE in the rice genome, was mobilized in transgenic rice plants [ 18 ].…”