Discovery of multiple beta-defensin like homologues in teleost fish

Zou, Jun; Mercier, Claire; Koussounadis, Antonis; Secombes, Christopher J.

doi:10.1016/j.molimm.2006.01.012

Cited by 186 publications

(160 citation statements)

References 37 publications

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“…Defensins from teleost fishes have been identified by molecular methodologies rather than purification of the native peptides in several fish species [112,[177][178][179][180][181]. Zhou et al [179] used EST and complete genome data to identify defensins from zebrafish (Dario rerio) and pufferfish (Takifugu rubripes) that resemble the β-defensins of birds and mammals.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

“…Zhou et al [179] used EST and complete genome data to identify defensins from zebrafish (Dario rerio) and pufferfish (Takifugu rubripes) that resemble the β-defensins of birds and mammals. Falco et al [182], using a recombinant protein based on rainbow trout defensin, found it to be antiviral against viral haemorrhagic septicaemia rhabdovirus (VHSV), one of the most troublesome diseases in fish aquaculture.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

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An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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Section: Antimicrobial Peptidesmentioning

confidence: 99%

Section: Antimicrobial Peptidesmentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

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“…The three subfamilies of defensins (␣, ␤, and ) found in mammals evolved from a common ancestral gene (10,11) whose ␤-defensin progeny extend back to bony fishes (12), if not beyond (13). All of these defensin peptides contain six cysteines, have three intramolecular disulfide bonds, and are cationic.…”

mentioning

confidence: 99%

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

Lehrer

Jung

Ruchala

et al. 2009

The Journal of Immunology

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Four of the six human α-defensins (human neutrophil peptides 1–3 and human α-defensin 5; HD5) have a lectin-like ability to bind glycosylated proteins. Using HD5 as a model, we applied surface plasmon resonance techniques to gain insights into this property. HD5 bound natural glycoproteins > neoglycoproteins based on BSA > nonglycosylated BSA ≫ free sugars. The affinity of HD5 for simple sugars covalently bound to BSA was orders of magnitude greater than its affinity for the same sugars in solution. The affinity of HD5 for protein-bound carbohydrates resulted from multivalent interactions which may also involve noncarbohydrate residues of the proteins. HD5 showed concentration-dependent self-association that began at submicromolar concentrations and proceeded to dimer and tetramer formation at concentrations below 5 μM. The (R9A, R28A) and (R13A, R32A) analogs of HD5 showed greatly reduced self-association as well as minimal binding to BSA and to BSA-affixed sugars. From this and other evidence, we conclude that the extensive binding of HD5 to (neo)glycoproteins results from multivalent nonspecific interactions of individual HD5 molecules with carbohydrate and noncarbohydrate moieties of the target molecule and that the primary binding events are magnified and enhanced by subsequent in situ assembly and oligomerization of HD5. Self-association and multivalent binding may play integral roles in the ability of HD5 to protect against infections caused by viruses and other infectious agents.

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“…In mammals, AMPs typically have broad-spectrum antimicrobial activity, they can often kill multiple pathogens that include bacteria, fungi, parasites and viruses. A large number of AMPs have been isolated from a wide number of fish species during last years, among which pleurocidin from winter flounder (Pleuronectes americanus) [2], cathelicidins from rainbow trout (Oncorhynchus mykiss) [3], defensins from zebrafish (Danio rerio) [4], piscidins from hybrid striped bass (white bass, Morone chrysops, female, x striped bass, Morone saxatilis, male) [5], dicentracin from sea bass (Dicentrarchus labrax) [6], and hepcidin from channel catfish (Ictalurus punctatus) [7], epinecidin from the grouper (Epinephelus coiodes) [8] (for a review see [9]). The activity of fish AMPs have been tested not only against the more common fish bacterial pathogens [8,10] but either against other pathogens like nervous necrosis virus [11].…”

Section: Open Accessmentioning

confidence: 99%

Importance of Fish Antimicrobial Peptides for Aquaculture and Biomedicine

Buonocore¹

2011

J Aquac Res Development

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Discovery of multiple beta-defensin like homologues in teleost fish

Cited by 186 publications

References 37 publications

An Overview of the Immunological Defenses in Fish Skin

An Overview of the Immunological Defenses in Fish Skin

Multivalent Binding of Carbohydrates by the Human α-Defensin, HD5

Importance of Fish Antimicrobial Peptides for Aquaculture and Biomedicine

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