Discovery of mosaic genomic islands in Pseudomonas spp.

Jani, Mehul; Azad, Rajeev K.

doi:10.1007/s00203-021-02253-2

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…In total, 10 genomic islands were annotated, of which 7 of them were longer than 15 kb. These repetitive sequences and genomic islands in bacterium are of great importance in the regulation of gene expression and acquiring of versatile characteristics [51]. This whole genome has been deposited in the DDBJ/ENA/GenBank under the accession number JBANFQ000000000.…”

Section: Genome Features and Mining Of Potential Cellulasesmentioning

confidence: 99%

Introduction of Cellulolytic Bacterium Bacillus velezensis Z2.6 and Its Cellulase Production Optimization

Cai,

Wang,

You

et al. 2024

Microorganisms

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Enzyme-production microorganisms typically occupy a dominant position in composting, where cellulolytic microorganisms actively engage in the breakdown of lignocellulose. Exploring strains with high yields of cellulose-degrading enzymes holds substantial significance for the industrial production of related enzymes and the advancement of clean bioenergy. This study was inclined to screen cellulolytic bacteria, conduct genome analysis, mine cellulase-related genes, and optimize cellulase production. The potential carboxymethylcellulose-hydrolyzing bacterial strain Z2.6 was isolated from the maturation phase of pig manure-based compost with algae residuals as the feedstock and identified as Bacillus velezensis. In the draft genome of strain Z2.6, 31 related cellulolytic genes were annotated by the CAZy database, and further validation by cloning documented the existence of an endo-1,4-β-D-glucanase (EC 3.2.1.4) belonging to the GH5 family and a β-glucosidase (EC 3.2.1.21) belonging to the GH1 family, which are predominant types of cellulases. Through the exploration of ten factors in fermentation medium with Plackett–Burman and Box–Behnken design methodologies, maximum cellulase activity was predicted to reach 2.98 U/mL theoretically. The optimal conditions achieving this response were determined as 1.09% CMC-Na, 2.30% salinity, and 1.23% tryptone. Validation under these specified conditions yielded a cellulose activity of 3.02 U/mL, demonstrating a 3.43-fold degree of optimization. In conclusion, this comprehensive study underscored the significant capabilities of strain Z2.6 in lignocellulolytic saccharification and its potentialities for future in-depth exploration in biomass conversion.

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Section: Genome Features and Mining Of Potential Cellulasesmentioning

confidence: 99%