Discovery of Fungal Denitrification Inhibitors by Targeting Copper Nitrite Reductase from <i>Fusarium oxysporum</i>

Matsuoka, Masaki; Kumar, Ashutosh; Muddassar, Muhammad; Matsuyama, Akihisa; Yoshida, Minoru; Zhang, Kam Y. J.

doi:10.1021/acs.jcim.6b00649

Cited by 29 publications

(16 citation statements)

References 75 publications

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“…These predominantly heterotrophic microorganisms are facultative anaerobes that are able to use NO 3instead of oxygen as an electron acceptor in respiration to cope with low oxygen or anaerobic conditions (Wrage et al, 20 | Wageningen Environmental Research report 2921 2001). Bacteria are mostly responsible for emission of N 2 O in soils (Syakila & Kroeze, 2011;Reay et al, 2012), however, fungi are also able to denitrify (Matsuoka et al, 2017). Nitrous oxide is a regular intermediate in denitrification which contrasts with nitrification where N 2 O is a by-product.…”

Section: Biological Denitrificationmentioning

confidence: 99%

Nitrous oxide emission from agricultural soils

Velthof

Rietra

2018

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2018. Nitrous oxide emission from agricultural soils. Wageningen, Wageningen Environmental Research, Report 2921. 58 pp.; 23 fig.; 19 tab.; 166 ref. Lachgas (N 2 O) is een broeikasgas met op mondiaal niveau een aandeel van 6% in de emissies van broeikasgassen. Bemeste landbouwgronden zijn een belangrijke bron van N 2 O. Er is een literatuurstudie uitgevoerd naar N 2 O-emissie uit landbouwgronden met focus op de emissie uit verschillende typen kunstmest en de mogelijkheden om de emissie uit kunstmest te beperken. De N 2 O-emissie uit kunstmest draagt voor 0,8% bij aan de totale broeikasgasemissie in Nederland. Uit de literatuur volgt dat toediening van kunstmest onder gemiddelde en relatief droge omstandigheden in de bodem leidt tot een beperkt risico op N 2 O-emissie voor zowel grasland als bouwland. Het risico op N 2 O-emissie neemt toe als nitraathoudende kunstmeststoffen, zoals kalkammonsalpeter en urean, onder natte omstandigheden aan grasland (alle grondsoorten) en bouwland (met name klei-en veengrond) worden toegediend. Het toedienen van een ammoniummeststof (eventueel met nitrificatieremmer) of ureum onder natte omstandigheden of het uitstellen van bemesting van de nitraathoudende kunstmest tot drogere omstandigheden zijn mogelijkheden om N 2 O-emissie te beperken. De zogenaamde 4R-strategie om de benutting van nutriënten uit meststoffen te verhogen, kan ook leiden tot een lagere N 2 O-emissie, namelijk N-toediening met het juiste type, juiste hoeveelheid, juiste tijdstip en juiste plaats.Nitrous oxide (N 2 O) is a greenhouse gas contributing to 6% of the global greenhouse effect. Fertilized agricultural soils are a major source of N 2 O. A literature study on N 2 O emission from agricultural soils was carried out focussing on the effect of different types of mineral nitrogen (N) fertilizers and strategies to mitigate N 2 O emission from fertilizers. The N 2 O emission from mineral N fertilizer application contributes to 0.8% of the total greenhouse gas emission in the Netherlands. The study shows that application of mineral N fertilizer under moderate and relatively dry conditions results in a relatively low risk of N 2 O emission from both grassland and arable land. However, during wet conditions, application of nitrate containing mineral fertilizers such as (calcium) ammonium nitrate and urean to grasslands (all soil types) and arable crops on clay and peat soils increases the risk of N 2 O emission. To decrease the risk of N 2 O emission during wet conditions, an ammonium based fertilizer (possibly with a nitrification inhibitor) or urea can be used. Alternatively, the application of nitrate based fertilizer should be postponed until drier periods. The 4R strategy to increase nutrient use efficiency of fertilizers will also reduce N 2 O emission, i.e., N application with the Right type, at the Right rate, at the Right time, and in the Right place.

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Section: Biological Denitrificationmentioning

confidence: 99%

Nitrous oxide emission from agricultural soils

Velthof

Rietra

2018

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“…As NO is already known as an inducer of dormancy in mycobacteria coupled with the reported fact that it could be produced from nitrite as well in several other bacterial species, we checked the production of NO in Mtb cells in the presence of nitrite in the medium 11 , 26 . In order to do this, Mtb cells were exposed to a cell permeable DAF2-DA dye, of which DAF2 specifically reacts with NO 27 , 28 to produce green fluorescence of triazofluorescein complex 29 . A significant difference in fluorescence intensity was observed when Mtb cells are exposed to (356 ± 11 relative fluorescent units (RFU)) nitrite (10 mM) compared to (43 ± 2 RFU) untreated control Mtb cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel gene similar to nitrite reductase (NO forming) plays potentially important role in the latency of tuberculosis

Agrawal

Gample

Yeware

et al. 2021

Sci Rep

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The development of the latent phenotype of Mycobacterium tuberculosis (Mtb) in the human lungs is the major hurdle to eradicate Tuberculosis. We recently reported that exposure to nitrite (10 mM) for six days under in vitro aerobic conditions completely transforms the bacilli into a viable but non-cultivable phenotype. Herein, we show that nitrite (beyond 5 mM) treated Mtb produces nitric oxide (NO) within the cell in a dose-dependent manner. Our search for the conserved sequence of NO synthesizing enzyme in the bacterial system identified MRA2164 and MRA0854 genes, of which the former was found to be significantly up regulated after nitrite exposure. In addition, the purified recombinant MRA2164 protein shows significant nitrite dependent NO synthesizing activity. The knockdown of the MRA2164 gene at mRNA level expression resulted in a significantly reduced NO level compared to the wild type bacilli with a simultaneous return of its replicative capability. Therefore, this study first time reports that nitrite induces dormancy in Mtb cells through induced expression of the MRA2164 gene and productions of NO as a mechanism for maintaining non-replicative stage in Mtb. This observation could help to control the Tuberculosis disease, especially the latent phenotype of the bacilli.

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“…The use of these inhibitors can also lead to less than desirable results: DMPP and 3-methylpyrazole 1,2,4-triazole (3MP + TZ) have been shown to increase N 2 O emissions in vegetable crop systems, as the inhibitors promote the buildup of N in the fraction of the soil most available to bacteria during the breakdown of vegetative matter. Synthetic denitrification inhibitors (SDIs) suppress denitrification via unknown mechanisms [82], although some are known to inhibit the activity of fungal copper reductase [83].…”

Section: Mechanism Of Action Pros Consmentioning

confidence: 99%

New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase

Demone

Wan

Nourimand

et al. 2018

Climate

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Nitrous oxide (N2O) is a potent greenhouse gas (GHG). Although it comprises only 0.03% of total GHGs produced, N2O makes a marked contribution to global warming. Much of the N2O in the atmosphere issues from incomplete bacterial denitrification processes acting on high levels of nitrogen (N) in the soil due to fertilizer usage. Using less fertilizer is the obvious solution for denitrification mitigation, but there is a significant drawback (especially where not enough N is available for the crop via N deposition, irrigation water, mineral soil N, or mineralization of organic matter): some crops require high-N fertilizer to produce the yields necessary to help feed the world’s increasing population. Alternatives for denitrification have considerable caveats. The long-standing promise of genetic modification for N fixation may be expanded now to enhance dissimilatory denitrification via genetic engineering. Biotechnology may solve what is thought to be a pivotal environmental challenge of the 21st century, reducing GHGs. Current approaches towards N2O mitigation are examined here, revealing an innovative solution for producing staple crops that can ‘crack’ N2O. The transfer of the bacterial nitrous oxide reductase gene (nosZ) into plants may herald the development of plants that express the nitrous oxide reductase enzyme (N2OR). This tactic would parallel the precedents of using the molecular toolkit innately offered by the soil microflora to reduce the environmental footprint of agriculture.

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Discovery of Fungal Denitrification Inhibitors by Targeting Copper Nitrite Reductase from Fusarium oxysporum

Cited by 29 publications

References 75 publications

Nitrous oxide emission from agricultural soils

Nitrous oxide emission from agricultural soils

Novel gene similar to nitrite reductase (NO forming) plays potentially important role in the latency of tuberculosis

New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase

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