Discovery of Four New Compounds from Macropanax membranifolius and Their Cytotoxic Activity

Abstract

A phytochemical investigation of the methanolic extract of the Macropanax membranifolius C.B. Shang leaves led to the isolation of three new flavans, (2R,3R)‐4′‐O‐methylcatechin 5‐O‐β‐D‐glucopyranoside (1), (2S,3S)‐4′‐O‐methylcatechin 5‐O‐β‐D‐glucopyranoside (2), (2S,3R)‐4′‐O‐methylcatechin 5‐O‐β‐D‐glucopyranoside (3), one new triterpene glycoside 3‐O‐β‐D‐xylopyranosyl‐(1→6)‐[β‐D‐xylopyranosyl‐(1→2)]‐β‐D‐glucopyranosyl‐oleanolic acid 28‐O‐β‐D‐glucopyranoside (4), together with nine known compounds (5‐13). Their chemical structures were elucidated based on HR‐ESI‐MS, NMR spectroscopic data. The absolute configurations of compounds 1‐3 were established by electronic circular dichroism (ECD) spectra. At concentration of 20 µM, compounds 1‐ 13 showed the percentages of dead cell in the range of 2.14% to 33.61% against KB, HepG2, HL60, P388, HT29, and MCF7 cancerous cell lines by SRB assay