Discovery of Differentially Expressed MicroRNAs in Porcine Ovaries With Smaller and Larger Litter Size

Xu, Guoqiang; Hu, Yamei; Yu, Dongling; Chen, Xingfa; Xiao, Li; Duan, Saixing; Zhang, Ning; Hu, Jianhong; Yang, Gongshe; Sun, Shiduo; Liu, Yong

doi:10.3389/fgene.2022.762124

Cited by 5 publications

(4 citation statements)

References 34 publications

(44 reference statements)

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“…The ovary is one of the important reproductive organs of female animals, regulating follicle development and hormone secretion ( 23 ). Proliferation and apoptosis are natural physiological processes that occur naturally during the growth and development of ovarian follicles ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of lncRNA and mRNA for the apoptosis of porcine ovarian granulosa cells after polyphenol resveratrol treatment

et al. 2023

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Resveratrol (RES) is a non-flavonoid polyphenol compound that can be involved in follicular development and ovulation. However, the mechanism by which resveratrol regulates the apoptosis of porcine ovarian granulosa cells (POGCs) through long non-coding RNA (lncRNA) is poorly understood. We generated POGCs models of different doses of RES (0, 25, 50, 75, and 100 μM). It was observed that the cell viability was the highest in the 50 μM group, and the highest apoptosis rates were recorded in the 100 μM group. Therefore, a control group (n = 3, 0 μM RES group), a low RES group (n = 3, 50 μM RES group), and a high RES group (n = 3, 100 μM RES group) of POGCs were created for next RNA sequencing. Gene Ontology (GO) indicated that differentially expressed lncRNAs associated with apoptotic process were highly enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of lncRNA target genes found that the Wnt signaling pathway and PI3K-Akt signaling pathway were both enriched. Furthermore, we constructed lncRNA-mRNA networks related to Metabolic and Cell Apoptosis, respectively. In the networks, five key-lncRNAs were screened, which may play a significant role in the process of POGCs metabolism and apoptosis. Furthermore, we focused on the function of a lnc-GAM (lncRNA associated with Granulosa cells Apoptosis and Metabolism) and verified that lnc-GAM could influence cell apoptosis in POGCs development by affecting the mRNA expression of apoptosis-related markers, and also affects the secretion of steroid hormones and related genes expression in POGCs cultured in vitro. Our study provides seminal data and important new insights into the regulation of reproductive mechanisms in porcine and other female mammals.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of lncRNA and mRNA for the apoptosis of porcine ovarian granulosa cells after polyphenol resveratrol treatment

et al. 2023

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“…Furthermore, miRNAs also have been found to play a pivotal role for the establishment of the proper uterine environment required for implantation [ 15 ]. Differentially expressed miRNAs in porcine ovaries have been identified as important regulators of the litter size [ 16 ]. However, there are few studies on endometrial miRNAs in the ovine fecundity.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

Dong,

Jiang,

Liu

et al. 2023

BMC Genomics

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Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of endometrial miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity, RNA-seq was used to comparatively analyze miRNA expression profiles of endometrium between high prolificacy sheep (HP, litter size = 3) and low prolificacy sheep (LP, litter size = 1) with FecB genotype. Results Firstly, genomic features of miRNAs from endometrium were analyzed. Furthermore, 58 differentially expressed (DE) miRNAs were found in the endometrium of Hu sheep with different litter size. A co-expression network of DE miRNAs and target genes has been constructed, and hub genes related litter size are included, such as DE miRNA unconservative_NC_019472.2_1229533 and unconservative_NC_019481.2_1637827 target to estrogen receptor α (ESR1) and unconservative_NC_019481.2_1637827 targets to transcription factor 7 (TCF7). Moreover, functional annotation analysis showed that the target genes (NRCAM and NEGR1) of the DE miRNAs were significantly enriched in cell adhesion molecules (CAMs) signaling pathway, which was related to uterine receptivity. Conclusion Taken together, this study provides a new valuable resource for understanding the molecular mechanisms underlying Hu sheep prolificacy.

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“…Furthermore, miRNAs also have been found to play a pivotal role for the establishment of the proper uterine environment required for implantation [14]. Differentially expressed miRNAs in porcine ovaries have been identi ed as important regulators of the litter size [15]. However, there are few studies on endometrial miRNAs in the ovine fecundity.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

Dong

Jiang

Liu

et al. 2023

Preprint

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Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of uterine miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity, RNA-seq was used to comparatively analyze miRNA expression profiles of endometrium between high prolificacy sheep (HP, litter size = 3) and low prolificacy sheep (LP, litter size = 1) with FecBB genotype. Results Firstly, genomic features of miRNAs from endometrium were analyzed. Furthermore, 58 differentially expressed (DE) miRNAs were found in the endometrium of Hu sheep with different litter size. A co-expression network of DE miRNAs and target genes has been constructed, and hub genes related litter size are included, such as DE miRNA unconservative_NC_019472.2_1229533 and unconservative_NC_019481.2_1637827 target to ESR1 and unconservative_NC_019481.2_1637827 targets to TCF7. Moreover, functional annotation analysis showed that the target genes (NRCAM and NEGR1) of the DE miRNAs were significantly enriched in cell adhesion molecules (CAMs) signaling pathway, which was related to uterine receptivity. Conclusion Taken together, this study provides a new valuable resource for understanding the molecular mechanisms underlying Hu sheep prolificacy.

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Discovery of Differentially Expressed MicroRNAs in Porcine Ovaries With Smaller and Larger Litter Size

Cited by 5 publications

References 34 publications

Integrated analysis of lncRNA and mRNA for the apoptosis of porcine ovarian granulosa cells after polyphenol resveratrol treatment

Integrated analysis of lncRNA and mRNA for the apoptosis of porcine ovarian granulosa cells after polyphenol resveratrol treatment

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity

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