Discovery of crystalline inclusions in <i>Bacillus licheniformis</i> that resemble parasporal crystals of <i>Bacillus thuringiensis</i>

Yan, Ming; Roehrl, Michael H.; Wang, Julia Y.

doi:10.1139/w07-076

Cited by 9 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 2003, some strains were transformed to express B. thuringiensis δ-endotoxin cry1Ab gene [40]. Furthermore, a newly discovered crystalline inclusion, that closely resembles the parasporal crystals of B. thuringiensis, suggests that it could be a gene transfer to them from B. thuringiensis [41]. The same study suggests that B. licheniformis adapts better naturally in phylloplane and that if potential insecticidal properties are discovered from B. licheniformis, they will make better candidates for insect biocontrol.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal Activity of Bacteria from Larvae Breeding Site with Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions

et al. 2020

View full text Add to dashboard Cite

Mosquitoes can transmit to humans devastating and deadly pathogens. As many chemical insecticides are banned due to environmental side effects or are of reduced efficacy due to resistance, biological control, including the use of bacterial strains with insecticidal activity, is of increasing interest and importance. The urgent actual need relies on the discovery of new compounds, preferably of a biological nature. Here, we explored the phenomenon of natural larvae mortality in larval breeding sites to identify potential novel compounds that may be used in biological control. From there, we isolated 14 bacterial strains of the phylum Firmicutes, most of the order Bacillales. Cultures were carried out under controlled conditions and were separated on supernatant and pellet fractions. The two fractions and a 1:1 mixture of the two fractions were tested on L3 and early L4 Aedes albopictus. Two concentrations were tested (2 and 6 mg/L). Larvae mortality was recorded at 24, 48 and 72 h and compared to that induced by the commercialized B. thuringiensis subsp. israelensis. Of the 14 strains isolated, 11 were active against the A. albopictus larvae: 10 of the supernatant fractions and one pellet fraction, and mortality increased with the concentration. For the insecticide activity prediction in three strains of the Bacillus cereus complex, PCR screening of the crystal (Cry) and cytolytic (Cyt) protein families characteristic to B. thuringiensis subsp. israelensis was performed. Most of the genes coding for these proteins’ synthesis were not detected. We identified bacterial strains that exhibit higher insecticidal activity compared with a commercial product. Further studies are needed for the characterization of active compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Insecticidal Activity of Bacteria from Larvae Breeding Site with Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Bt biopesticide is as effective as chemical pesticides and fits within the pest management approach, which supports non-chemical pesticides (Matyjaszczyk 2018). Bt can produce insecticidal toxin proteins such as crystalline, proteinaceous (Yan, et al, 2007), and antifungal toxin Yvgo protein (Manns, et al, 2012). Most strains of Bt can produce cry toxins, which are responsible for the toxic effect (Rubio & Moreno, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mammalian Biosafety of Bacillus thuringiensis subsp. kurstaki -Based Bioinsecticide

El-Saadany

Kotb

Ibrahim

et al. 2022

Egyptian Academic Journal of Biological Sciences, B. Zoology

View full text Add to dashboard Cite

Bacillus thuringiensis subsp. kurstaki (Btk) is well-known and widely used as a biological insecticide. Registration of biopesticides with the national pesticides broad must be laboratory testing for acute toxicity as short-term and long-term health effects. Acute oral toxicity tests were carried out on rats with -endotoxin and formulation (9.4% WP) of Btk based on the Organization for Economic Co-operation and Development (OECD). The purpose of this study is to assess the biosafety of the bioinsecticide Btk (-endotoxin and 9.4% WP) in adult rats after a single oral dose based on hematotoxicity, hepato-renal toxicity, and the lipid profile. Results illustrate that most haematological parameters increased in treated rats. Compared to the control, liver enzymes (AST, ALT, and ALP) and globulin (Glb) concentration were increased and decreased in male rats subjected to both kinds of Btk. While female rats treated with both Btk products are showing a significant elevation in ALP, total protein (TP), albumin (Alb), and Glb levels versus the levels of ALT and AST. Urea concentrations decreased significantly in all treated rats, but Creatinine levels appeared to remain unchanged. Moreover, there was a decline and elevation in the serum of lipid profiles (T cholesterol and HDL) in treated male rats and female rats, respectively. LDL levels increased in treated male animals, while they decreased in treated females. All treated animals displayed negligible modifications in tissue somatic index, except a slight increase in lung weights when compared to the control. Contradictory, the body weight gain levels were reduced in all treated animals compared to untreated. In addition, there are no mortality or pathogenicity symptoms in all treated animals. These findings suggest that both treatments can induce hepato-renal toxicity and hematotoxicity with side effects on lipid profile aspects. Therefore, additional research, including subacute and chronic studies, is recommended.

show abstract

“…2 This biopesticides can produce crystalline, proteinaceous and delta-endotoxin, which are highly toxic for different types of agricultural pests. 3,4 The B. thuringiensis toxins have massive potential for the control of agricultural diseases. 5 They target a limited spectrum of pest insects that contain specific physiological properties (i.e., gut pH and toxin receptor sites in the midgut) and eventually have low risk to non-target species than broad-spectrum insecticides.…”

Section: Introductionmentioning

confidence: 99%

Toxicological Examinations Following Oral Administration of Bacillus thuringiensis var kurstaki Biological Insecticide in Wistar Rats

Larki

Zayerzadeh²,

Harzandi

et al. 2019

J Apple Biotechnol Rep

View full text Add to dashboard Cite

Introduction: Bacillus thuringiensis var kurstaki is one of the best known biological insecticide which is used extensively in the world. This biopesticide has been broadly used against important insect pests and vectors of animals and humans. However, the controversial data was published to date considering toxicological studies on non-target species are urgent to determine probable adverse effects and risk assessment of this biopesticide. In this research, histopathological changes, hematological and some biochemical factors were evaluated following the single dose oral administration of B. thuringiensis in rats. Materials and Methods: Twelve Wistar rats were randomly divided into 2 groups including experimental and control groups. Animals were treated orally by gavage to single dose of sub-lethal dose (5000 mg/kg) of B. thuringiensis suspension. Finally, hematological factors (WBC, RBC, Hb, HCT, MCV, MCH, MCHC and PLT), some biochemical parameters (ALT, AST, ALP, BUN and Cr) and histopathological observations were evaluated. Results: The results demonstrated that oral administration of high dose of B. thuringiensis biopesticide induced some pathological complications including congestion and inflammation in vital organs of rats such as liver, heart, lung and kidney. It also caused hematological abnormalities and biochemical alterations in the experimental animals group. Conclusions: According to the findings, it can be concluded that high dose of B. thuringiensis biopesticide can induce toxicity in rats. Therefore, further investigations including subacute and chronic are recommended.

show abstract

Discovery of crystalline inclusions in Bacillus licheniformis that resemble parasporal crystals of Bacillus thuringiensis

Cited by 9 publications

References 16 publications

Insecticidal Activity of Bacteria from Larvae Breeding Site with Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions

Insecticidal Activity of Bacteria from Larvae Breeding Site with Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions

Mammalian Biosafety of Bacillus thuringiensis subsp. kurstaki -Based Bioinsecticide

Toxicological Examinations Following Oral Administration of Bacillus thuringiensis var kurstaki Biological Insecticide in Wistar Rats

Contact Info

Product

Resources

About