Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi

He, Min; Su, Jia; Xu, Youpin; Chen, Jinhua; Chern, Mawsheng; Lei, Mingliang; Qi, Tuo; Wang, Zongkuan; Ryder, Lauren S.; Tang, Bozeng; Osés-Ruiz, Míriam; Zhu, Keke; Cao, Yuyan; Xia, Yan; Eisermann, Iris; Luo, Yuan; Li, Weitao; Wang, Jing; Yin, Junjie; Lam, Sin Man; Peng, Guoxiong; Sun, Xin; Zhu, Xiaobo; Ma, Bo; Liu, Jiali; Qing, Hai; Li, Song; Wang, Long; Hou, Qingqing; Peng, Qin; Li, Yan; Fan, Jing; Li, Deqiang; Wang, Yuping; Wang, Xiue; Jiang, Ling; Shui, Guanghou; Xia, Yuxian; Gong, Guoshu; Huang, Fu; Wang, Wenming; Wu, Xiaoyun; Li, Ping; Zhu, Lihuang; Li, Shigui; Talbot, Nicholas J.; Chen, Xuewei

doi:10.1038/s41564-020-00790-y

Cited by 49 publications

(60 citation statements)

References 53 publications

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“…1a). This dome-shaped, darkly melanized cell directs a high internal turgor onto a needle-like penetration peg [14][15][16] , which emerges from a 2-500 nm pore at the appressorial base to puncture the plant's outer defensive barriers, the cuticle and epidermal cell wall. Despite major advances in our understanding of the cellular processes preceding 11,12,[16][17][18][19] and following 20,21 plant cell entry, the (bio)chemical reactions occurring at this nanoscale plant-fungus interface and their role in host penetration remain poorly understood.…”

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confidence: 99%

Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens

Bissaro

Kodama

Hage

et al. 2021

Preprint

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Copper radical oxidases (CRO) form a class of enzymes with a longstanding history encompassing diverse substrate specificities. While the biological function of most CROs remains unknown, we observed that CROs active on aliphatic alcohols are found only in fungal plant pathogens. Here, we unveil the role of these CROs and the identity of their natural redox partner, a haem-iron peroxidase. Combining multiscale approaches, we report that Colletotrichum and Magnaporthe appressoria (specialized cells that puncture the plant cuticles) co-secrete this pair of metalloenzymes early during penetration. We show in vivo that mutant appressoria lacking either or both enzymes have impaired penetration ability and pathogenicity. We reveal in vitro a finely-tuned enzyme interplay is responsible for the oxidation of plant cuticular long-chain alcohols into aldehyde products, suggested to act as key molecular signals in the fungal infection machinery. Our results open new avenues to design oxidase-specific inhibitors as anti-penetrants for crop protection.

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confidence: 99%

Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens

Bissaro

Kodama

Hage

et al. 2021

Preprint

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“…35 Rice blast, a worldwide rice disease caused by Magnaporthe grisea, may occur in the whole growth period of rice, resulting in 11-30% loss of rice yield every year. 39,40 At present, 33 rice blast resistance genes have been cloned, including Pit, Pi37, Pish, Pib,…”

Section: Introductionmentioning

confidence: 99%

“…Rice blast, a worldwide rice disease caused by Magnaporthe grisea , may occur in the whole growth period of rice, resulting in 11–30% loss of rice yield every year 39,40 . At present, 33 rice blast resistance genes have been cloned, including Pit , Pi37 , Pish , Pib , bsr‐d1 , Pi21 , Pi63/Pikahei‐l(t) , Pi2/Piz‐t/Pi2‐1/Pi2‐2/Pi9/Pigm , Pi‐d3/Pi25(t) , Pi‐d2 , Pi36 , Pi5 , Pi54/Pi‐k h , Pb1 , Pik/Pik‐h/Pik‐m/Pik‐p/Pik‐s/Pi1/Pik‐g(t) , Pia/PiCO39 , Pi‐ta and Ptr 41–68 .…”

Section: Introductionmentioning

confidence: 99%

Development of ‘multiresistance rice’ by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system

Zhang

Ren

et al. 2021

Pest Management Science

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Background Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. Results In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate‐tolerance gene (I. variabilis‐EPSPS*), lepidopteran pest resistance gene (Cry1C*), brown planthopper resistance genes (Bph14* and OsLecRK1*), bacterial blight resistance gene (Xa23*) and rice blast resistance gene (Pi9*) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium‐mediated transformation and ‘multiresistance rice’ (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. Conclusion A multigene transformation strategy was employed to successfully develop rice lines with multiresistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast, and the obtained MRR is expected to have great application potential. © 2020 Society of Chemical Industry

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“…The ring structures appeared at the mother-bud neck in yeast [ 20 ] and the appressorium-penetration peg neck in rice blast fungus associated with the curved membrane and virulence [ 21 ]. New research demonstrates that septin proteins bind phosphatidylinositol phosphates (pips) at the appressorium pore membrane to assemble into a ring, promoting the formation of a penetration peg required for host infection in M. oryzae [ 22 ]. In addition to filaments and rings, septins can also form structures referred to as gauzes, which consist of a meshwork of septin filaments in yeast [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Insights of roles played by septins in pathogenic fungi

Lin

Zhu

et al. 2021

Virulence

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Septins, a conserved family of GTP-binding proteins, are widely recognized as an essential cytoskeletal component, playing important roles in a variety of biological processes, including division, polarity, and membrane remodeling, in different eukaryotes. Although the roles played by septins were identified in the model organism Saccharomyces cerevisiae , their importance in other fungi, especially pathogenic fungi, have recently been determined. In this review, we summarize the functions of septins in pathogenic fungi in the cell cycle, autophagy, endocytosis and invasion host-microbe interactions that were reported in the last two years in the field of septin cell biology. These new discoveries may be expanded to investigate the functions of septin proteins in fungal pathogenesis and may be of wide interest to the readers of Microbiology and Molecular Pathology.

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Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi

Cited by 49 publications

References 53 publications

Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens

Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens

Development of ‘multiresistance rice’ by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system

Insights of roles played by septins in pathogenic fungi

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