Discovery of a Thermostable Tagatose 4-Epimerase Powered by Structure- and Sequence-Based Protein Clustering
JiaJun Chen,
Dawei Ni,
Yingying Zhu
et al.
Abstract:d-Tagatose is a highly promising functional
sweetener
known for its various physiological functions. In this study, a novel
tagatose 4-epimerase from Thermoprotei archaeon (Thar-T4Ease),
with the ability to convert d-fructose to d-tagatose,
was discovered through a combination of structure similarity search
and sequence-based protein clustering. The recombinant Thar-T4Ease
exhibited optimal activity at pH 8.5 and 85 °C, in the presence
of 1 mM Ni2+. Its k
cat and k
cat/K
m values toward d-fructose were measure… Show more
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