Discovery of a new species within Bacillus genus in a stool sample from Gabon: “Bacillus massiliogabonensis” sp. nov.

Mourembou, Gaël; Ndjoyi-Mbiguino, Angélique; Lékana-Douki, Jean Bernard; Fournier, Pierre‐Edouard; Raoult, Didier; Bittar, Fadi

doi:10.1016/j.nmni.2016.09.013

Cited by 3 publications

(2 citation statements)

References 11 publications

(15 reference statements)

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“…The gut is analysed mainly by culture-based methods [15]. In this study, examining 10 [16,17,18,19]. Other studies have also reported the presence of Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus in human faeces [20,21,22].…”

Section: Discussionmentioning

confidence: 98%

Phylogenetic Biodiversity of Bacteria Community in the Gut of Diarrhoeic Patients in Rivers State, Nigeria

Agi

Azike

2019

JAMB

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Background: The microbial ecosystem in the human intestine is complex and it plays a great role in health and nutrition. Cultural techniques have been used over the years to study the gut microbiota but studies suggest that a greater percentage of these bacteria found in the gut cannot be cultivated using the conventional methods of bacteria isolation. Aim: To increase understanding in this area, we characterized the bacterial diversity (both cultivated and non-cultivated bacteria) in the gut of diarrhoeic individuals using 16S rRNA gene (rDNA) sequences. Methodology: PCR amplification, sequencing and phylogenetic analysis of the 16S ribosomal DNA (rDNA) sequences were done on 10 diarrhoeic stool samples. Results: After quality filtering and chimeric sequence removal, 72313 sequences from all 10 diarrhoeic stool samples subjected to clustering generated 2767 Operational Taxonomic Units (OTUs) of which 2073 were new and unassigned. Representative sequences of the bacteria OTUs cluster were used to construct a bacteria phylogenetic tree which revealed a wide variety of bacteria Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Tenericutes and Cyanobacteria and others that could not be detected using the cultural techniques. The evolutionary relationship of the most abundant organisms and their contributions from each sample revealed the phylum Firmicutes to be most abundant and therefore have contributed most in the samples followed by Bacteroidetes. Fewer contributions were made by the other phyla Proteobacteria, Actinobacteria, Tenericutes and Cyanobacteria. Conclusion: This study was able to identify culturable and unculturable bacteria in the gut of diarrhoeic people in Rivers state and also show the biodiversity and interrelatedness of these microorganisms using molecular methods. Therefore, we can say that 16S rRNA techniques for detection and identification of predominant bacteria create new opportunities for non-cultivation studies of the human intestinal microflora, proper diagnosis of infectious diseases and new methods of treatments of diseases.

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Section: Discussionmentioning

confidence: 98%

Phylogenetic Biodiversity of Bacteria Community in the Gut of Diarrhoeic Patients in Rivers State, Nigeria

Agi

Azike

2019

JAMB

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“…A culturing approach that uses high-throughput culture conditions in combination with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and 16S rRNA sequencing for taxonomic identification and referred to as culturomics has allowed significant increase in a number of bacteria discovered in human GIT including species known in humans but not in the gut, species previously considered unculturable as well as new species. Considerable part of them is represented by Firmicutes ( Lagier et al, 2016 ) including Bacillus species ( Lagier et al, 2015 ; Mourembou et al, 2016 ; Senghor et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Secretome of Intestinal Bacilli: A Natural Guard against Pathologies

et al. 2017

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Current studies of human gut microbiome usually do not consider the special functional role of transient microbiota, although some of its members remain in the host for a long time and produce broad spectrum of biologically active substances. Getting into the gastrointestinal tract (GIT) with food, water and probiotic preparations, two representatives of Bacilli class, genera Bacillus and Lactobacillus, colonize epithelium blurring the boundaries between resident and transient microbiota. Despite their minor proportion in the microbiome composition, these bacteria can significantly affect both the intestinal microbiota and the entire body thanks to a wide range of secreted compounds. Recently, insufficiency and limitations of pure genome-based analysis of gut microbiota became known. Thus, the need for intense functional studies is evident. This review aims to characterize the Bacillus and Lactobacillus in GIT, as well as the functional roles of the components released by these members of microbial intestinal community. Complex of their secreted compounds is referred by us as the “bacillary secretome.” The composition of the bacillary secretome, its biological effects in GIT and role in counteraction to infectious diseases and oncological pathologies in human organism is the subject of the review.

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fosM , a New Family of Fosfomycin Resistance Genes Identified in Bacterial Species Isolated from Human Microbiota

Khabthani

Hamel

Baron

et al. 2021

Antimicrob Agents Chemother

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Fosfomycin is a decades-old antibiotic, currently reused because of its activity against multidrug resistant bacteria. Here, we used a combined in vitro/in silico approach to search for fosfomycin resistance determinants in 25 new bacterial species isolated from the human microbiota. Putative resistance genes were cloned into a susceptible Escherichia coli. Minimal Inhibitory Concentration (MIC) values increased from 1 μg/ml to 1024 μg/ml. We report here new family of potential chromosomal fosfomycin resistance genes named fosM.

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Discovery of a new species within Bacillus genus in a stool sample from Gabon: “Bacillus massiliogabonensis” sp. nov.

Cited by 3 publications

References 11 publications

Phylogenetic Biodiversity of Bacteria Community in the Gut of Diarrhoeic Patients in Rivers State, Nigeria

Phylogenetic Biodiversity of Bacteria Community in the Gut of Diarrhoeic Patients in Rivers State, Nigeria

Secretome of Intestinal Bacilli: A Natural Guard against Pathologies

fosM , a New Family of Fosfomycin Resistance Genes Identified in Bacterial Species Isolated from Human Microbiota

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