Discovery of a New and Efficient Small Molecule for Neuronal Differentiation from Mesenchymal Stem Cell

Ri, Kim， Na; Kang, Seung Kyu; Ahn, Hyun Hee; Kwon, Sung Wook; Park, Woul Seong; Kim, Ki Suk; Kim, Sung Soo; Jung, Hee Jung; Choi, Sang Un; Ahn, Jin Hee; Kim, Kwang-Rok

doi:10.1021/jm9015558

Cited by 15 publications

(14 citation statements)

References 21 publications

(22 reference statements)

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“…The fate of HSCs is regulated by small molecules that promote self-renewal [181][182][183] . Using small molecules, multipotent MSCs can differentiate into various non-hematopoietic cells, such as chondrocytes [134,184] , osteoblasts [185,186] , hepatocytes [187] , cardiomyocytes [188,189] , adipocytes [190,191] and neuronal-like cells [192][193][194] . Additionally, the maintenance of MSCs is associated with the Wnt signaling pathway [195,196] .…”

Section: Small Molecules and Stem Cell Fatementioning

confidence: 99%

MicroRNAs as novel regulators of stem cell fate

Choi

Hwang³

2013

WJSC

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Mounting evidence in stem cell biology has shown that microRNAs (miRNAs) play a crucial role in cell fate specification, including stem cell self-renewal, lineagespecific differentiation, and somatic cell reprogramming. These functions are tightly regulated by specific gene expression patterns that involve miRNAs and transcription factors. To maintain stem cell pluripotency, specific miRNAs suppress transcription factors that promote differentiation, whereas to initiate differentiation, lineagespecific miRNAs are upregulated via the inhibition of transcription factors that promote self-renewal. Small molecules can be used in a similar manner as natural miRNAs, and a number of natural and synthetic small molecules have been isolated and developed to regulate stem cell fate. Using miRNAs as novel regulators of stem cell fate will provide insight into stem cell biology and aid in understanding the molecular mechanisms and crosstalk between miRNAs and stem cells.Ultimately, advances in the regulation of stem cell fate will contribute to the development of effective medical therapies for tissue repair and regeneration. This review summarizes the current insights into stem cell fate determination by miRNAs with a focus on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.© 2013 Baishideng. All rights reserved. Key words:MicroRNA; Stem cell fate; Differentiation; Self-renewal; Reprogramming; Small molecule Core tip: Stem cells are important in regenerative medicine applications due to their capacity to self-renew and differentiate into specific cell types. MicroRNAs (miRNAs) are short non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. Recent studies suggest that miRNAs are key molecules in the regulation of stem cell fate decisions; this regulation is manifested as the fine tuning of cell-and tissue-specific gene expression. This review summarizes the current insights into stem cell fate determination by miRNAs and focuses on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.

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Section: Small Molecules and Stem Cell Fatementioning

confidence: 99%

MicroRNAs as novel regulators of stem cell fate

Choi

Hwang³

2013

WJSC

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“…To induce neuronal differentiation, 5 3 10 4 cells were grown on glass slides in 24-well culture dishes and incubated with 40 lM neuronal inducer TCS 2210 (Tocris, Boston, MA, USA) in standard medium as described previously. 17 Neuronal differentiation was observed by detection of ß3-tubulin (1:200, rabbit monoclonal IgG; Cell Signaling Technology, Danvers, MA, USA) in combination with a secondary antibody goat anti-rabbit IgG-Alexa Fluor 594 (1:400; Invitrogen) and 4 0 ,6-diamidino-2phenylindol (DAPI) counterstaining of nuclei using immunofluorescence microscopy. 7…”

Section: Neuronal Differentiationmentioning

confidence: 99%

Orbital Fibroblasts From Graves' Orbitopathy Patients Share Functional and Immunophenotypic Properties With Mesenchymal Stem/Stromal Cells

Brandau

Bruderek

Hestermann

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…Drug discovery based on these cells is likely to become a main stream component of all pharmaceutical discovery strategies in the near future [77][78][79][80]. Indeed MSC based approaches are already in use [81][82][83] and have major advantages over conventional non-candidate based hit identification programs. MSCs are not transformed, have less restricted lineage limitations, and display more relevant growth/ cell division characteristics [7,59].…”

Section: Advantages Of Msc Based Drug Discovery Platformmentioning

confidence: 99%

Mesenchymal Stromal Cells; Role in Tissue Repair, Drug Discovery and Immune Modulation

English¹,

Mahon²,

Wood³

2013

CDD

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Mesenchymal stromal cells (MSCs) participate in repair of damaged tissues, possess the potential to serve as a useful tool in the drug discovery field and exert immunosuppressive effects as demonstrated by their ability to modulate the immune response. Herein, the roles played by MSC differentiation and/or production of trophic factors involved in tissue repair are discussed. MSCs offer the opportunity to probe targets that conventional or differentiated cell lines do not express; thus providing a more refined system that allows identification of novel therapeutics. However, there are difficulties associated with drug discovery assays to which MSCs are not exempt. The immunosuppressive potential of MSCs has already been utilised in clinical trials where MSCs have been used to treat patients with graft-versus-host disease (GvHD) and autoimmune diseases. Another possible therapeutic application of MSCs lies in the field of transplantation tolerance. Although the capacity of MSCs to modulate immune responses has received much attention, the role of MSCs in transplantation tolerance is as yet unclear. In this review, we discuss the evidence for MSC induction of a state of tolerance in the transplantation setting.

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Discovery of a New and Efficient Small Molecule for Neuronal Differentiation from Mesenchymal Stem Cell

Abstract: A new synthetic small molecule, compound 1, which induced a neuronal differentiation from mesenchymal stem cells (MSCs) with an excellent efficiency, was identified. Furthermore the differentiated cell by 1 showed the neural electrophysiological and cholinergic neuron properties.

Cited by 15 publications

References 21 publications

MicroRNAs as novel regulators of stem cell fate

MicroRNAs as novel regulators of stem cell fate

Orbital Fibroblasts From Graves' Orbitopathy Patients Share Functional and Immunophenotypic Properties With Mesenchymal Stem/Stromal Cells

Mesenchymal Stromal Cells; Role in Tissue Repair, Drug Discovery and Immune Modulation

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