Discovery in Droplets

Droplet microfluidics for microbiology: techniques, applications and challenges

“…48,49 Detailed reviews of the techniques for handling droplets have been extensively presented in multiple publications recently. [50][51][52][53] From the point of view of usage in microbiology it is important to know that it is possible to manipulate both small (from a few microliters) and large (mL and bigger) volumes of aqueous suspensions of microorganisms. These can be split into droplets ranging in volume from pico-to microliters.…”

Separation of main and satellite droplets in a deterministic lateral displacement microfluidic device

“…8c). In each DLD region, the main and satellite droplets trajectories correspond with the trajectories (displacement mode or zigzag mode) given by the critical diameter calculated using eqn (1).…”

“…Microuidic droplet-generating technology is used in a growing number of applications such as droplet-based bioassays [1][2][3] including digital polymerase chain reaction (PCR) 4 and singlecell analysis, 5,6 and functional microparticles production [7][8][9][10] because it offers unprecedented ne control over size, structural and/or chemical compositions, and spatio-temporal arrangement of the produced droplets. To date, a number of studies have been conducted using a microuidic droplet generator (MFDG) such as T-shaped and ow focusing microchannels on a chip or three-dimensional coaxial microcapillary congurations.…”

From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis

“…In the current review, we aim to survey recent developments in the use of droplet-based microfluidics for nucleic acid analysis, first highlighting key areas where such microfluidic tools have had significant effect and secondly proposing related applications where microfluidic technologies may have impact in the short to medium term. We also note that although essential background knowledge, such as the manner in which droplets are formed and manipulated, will be introduced, more detailed and comprehensive analyses of droplet-based microfluidic systems can be found elsewhere (Niu and deMello 2012;Oh et al 2012;Choi et al 2012;Baroud et al 2010;Kelly et al 2007;Shembekar et al 2016;Price and Paegel 2016;Collins et al 2015).…”