Discovery, Expression, and In Silico Safety Evaluation of Honey Truffle Sweetener, a Sweet Protein Derived from Mattirolomyces terfezioides and Produced by Heterologous Expression in Komagataella phaffii
Chase McFarland,
Bassam Alkotaini,
Chloe P. Cowen
et al.
Abstract:Honey truffle sweetener (HTS), a
121 amino acid protein is identified
as a high-intensity sweetener found naturally occurring in the Hungarian
Sweet Truffle Mattirolomyces terfezioides, an edible mushroom used in regional diets. The protein is intensely
sweet, but the truffle is difficult to cultivate; therefore, the protein
was systematically characterized, and the gene coding for the protein
was expressed in a commonly used host yeast Komagataella
phaffii. The heterologously expressed protein maintained
the … Show more
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