Discovery and engineering of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products

Abstract

The major approaches (i.e. bioactivity-guided screening, genome mining, and biosynthetic engineering) employed in the exploration of RiPP (ribosomally synthesized and post-translationally modified peptides) natural products are discussed in this review.

Late-Stage Formation of a Sactionine Linkage Enabled by Lossen Rearrangement of Glycyl Hydroxamic Acid

“…R ecent advances in genome mining technology have facilitated the discovery of numerous ribosomally synthesized and post-translationally modified peptides (RiPPs) produced by various bacteria, leading to major breakthroughs in natural product chemistry. 1 Such RiPPs include members of the sactipeptide (sulfur-to-alpha carbon thioether cross-linked) family, which feature a sactionine thioether linkage between the sulfur of a Cys residue and the α-carbon of another amino acid residue (Figure 1). 2,3 Because sactipeptides exhibit antibacterial activity, they have the potential to serve as antibiotics.…”