Discovery and Current Status of Evaluation System of Bioavailability and Related Pharmaceutical Technologies for Traditional Chinese Medicines—Flos Lonicerae Japonicae—Fructus Forsythiae Herb Couples as an Example

Cai, Baochang; Shan, Jinjun; Shouchuan, Wang; Di, Liuqing

doi:10.3390/ijms161226132

Cited by 12 publications

(9 citation statements)

References 181 publications

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“…Because a high-degree chemical similarity does not equal a bioequivalence. Therefore, the establishment of a complete system for the determination of bioavailability using biological techniques is the key factor in the assessment of consistency of quality and the modernization of natural herbal medicines [117,118].…”

Section: Biological Consistency Evaluation Methodsmentioning

confidence: 99%

Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations

et al. 2020

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Quality consistency is one of the basic attributes of medicines, but it is also a difficult problem that natural medicines and their preparations must face. The complex chemical composition and comprehensive pharmacological action of natural medicines make it difficult to simply apply the commonly used evaluation methods in chemical drugs. It is thus urgent to explore the novel evaluation methods suitable for the characteristics of natural medicines. With the rapid development of analytical techniques and the deepening understanding of the quality of natural herbs, increasing numbers of researchers have proposed many new ideas and technologies. This review mainly focuses on the basic principles, technical characteristics and application examples of the chemical evaluation, biological evaluation methods and their combination in quality consistency evaluation of natural herbs. On the bases of chemical evaluation and clinical efficacy, new methods reflecting their pharmacodynamic mechanism and safety characteristics will be developed, and gradually towards accurate quality control, to achieve the goal of quality consistency. We hope that this manuscript can provide new ideas and technical references for the quality consistency of natural drugs and their preparations, thus better guarantee their clinical efficacy and safety, and better promote industrial development.

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Section: Biological Consistency Evaluation Methodsmentioning

confidence: 99%

Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations

et al. 2020

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“…122 The quantity of NOc fabrication at 3 to 25 mM was continuously calculated, and the IC 50 values were found to be 12.4 and 10.1 mM for aand , and human neuroblastoma cells (SH-SY5Y). 131,132 The modied xanthones were found to exhibit the most potent antitumor activities by inducing apoptosis in HepG2 cell lines in a dose-dependent manner. 133 It was found that the efficiency of cell growth inhibition increased dramatically when the concentration of modied xanthones was increased.…”

Section: Xanthones and Xanthene-based Scaffoldsmentioning

confidence: 99%

“…133 It was found that the efficiency of cell growth inhibition increased dramatically when the concentration of modied xanthones was increased. 131,132 Jang et al reported that modied xanthones (Fig. 26) were selective agonist for TrkA receptor, showing a strong neurotrophic activity by selectively binding to TrkA, inducing its tyrosine phosphorylation, provoking outgrowth in PC12 cells, eliciting PI3-kinase/Akt and MAPK activation, thus preventing neuronal cell death.…”

Section: Xanthones and Xanthene-based Scaffoldsmentioning

confidence: 99%

The value of pyrans as anticancer scaffolds in medicinal chemistry

et al. 2017

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“…Overall, PADP has positive effect on inhibiting the growth of cancer cells in patients with liver cancer, alleviating clinical symptoms of patients, improving their living quality, and increasing the survival period of patients. Due to the complex composition of traditional Chinese medicines ( 8 ), it is difficult for traditional pharmacological research methods to systematically explain the mechanism of action and signaling pathways of PADP on the treatment of liver cancer. Therefore, the mechanism of PADP on the treatment of liver cancer has not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Phellodendron and Anemarrhena Drug Pair on the Treatment of Liver Cancer Based on Network Pharmacology and Bioinformatics

Ruan

et al. 2022

Front. Oncol.

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BackgroundThis study aims to explore the key targets and signaling pathways of the traditional Chinese medicine Phellodendron and Anemarrhena drug pair (PADP) for the treatment of liver cancer.MethodsFirstly, bioinformatics technology was used to analyze GSE62232 gene chip to obtain the differential genes of liver cancer. A network pharmacology technology was used to find the active components of PADP and their targets. Secondly, the differential genes were imported into STRING database to draw a PPI network, and network topology structure map combined with Cytoscape software. And the R language was used to identify differential gene targets and pathways through GO and KEGG pathway enrichment analysis. In addition, AutoDock Vina was used for molecular docking of core targets and core compounds. Moreover, GEPIA online analysis tool was used to perform survival analysis of the core target genes. Finally, RT-PCR was used to verify the changes of key target genes. CCK−8 assay was performed to detect cell proliferation. Flow cytometry was performed to detect the cell cycle and apoptotic. Transwell invasion assay was performed to detect cell invasion.ResultsFirstly, a total of 21,654 genes were obtained. After screening, 1019 differential genes were obtained, including 614 down-regulated genes and 405 up-regulated genes. Furthermore, after screening by ADME standards, 52 active ingredients were obtained, of which 37 were Phellodendron and 15 were Anemarrhena. And a total of 36 differential genes have been identified, including 13 up-regulated genes and 23 down-regulated genes. Moreover, through enrichment analysis, we found that PADP may treat liver cancer through multiple channels and multiple pathways including the p53 signaling pathway, IL-17 signaling pathway, TNF signaling pathway, Toll-like receptor signaling pathway and so on. Secondly, the molecular docking results showed that there was certain affinity between the core compounds and core target genes. In addition, GEPIA online analysis showed that ESR1, AR, CCNB1, CDK1, AKR1C3 and CCNA2 might become potential target genes for the survival and prognosis of PADP for the treatment of liver cancer. Finally, it was found that PADP could up regulate genes ESR1 and AR, down regulate genes CCNB1, CDK1, AKR1C3, and CCNA2. PADP could promote the apoptosis of liver cancer cells, shorten the cell cycle, and inhibit the proliferation and invasion of liver cancer cells.ConclusionPADP may treat liver cancer through multiple targets, multiple channels, and multiple pathways, thereby suppressing cancer cells and improving the living quality of patients.

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Discovery and Current Status of Evaluation System of Bioavailability and Related Pharmaceutical Technologies for Traditional Chinese Medicines—Flos Lonicerae Japonicae—Fructus Forsythiae Herb Couples as an Example

Cited by 12 publications

References 181 publications

Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations

Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations

The value of pyrans as anticancer scaffolds in medicinal chemistry

Mechanism of Phellodendron and Anemarrhena Drug Pair on the Treatment of Liver Cancer Based on Network Pharmacology and Bioinformatics

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