Discovery and characterization of EII B, a new α-conotoxin from Conus ermineus venom by nAChRs affinity capture monitored by MALDI-TOF/TOF mass spectrometry

Echterbille, Julien; Gilles, Nicolas; Aráoz, Rómulo; Mourier, Gilles; Amar, Muriel; Servent, Denis; Pauw, Edwin De; Quinton, Loïc

doi:10.1016/j.toxicon.2017.02.023

Cited by 10 publications

(10 citation statements)

References 42 publications

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“…2004 ; Rivera-Ortiz et al. 2011 ; Echterbille et al. 2017 ) and some conotoxin precursors belonging to the A, B1, and O1 superfamilies ( Duda and Palumbi 2004 ; Gowd et al.…”

Section: Introductionmentioning

confidence: 99%

Conotoxin Diversity in Chelyconus ermineus (Born, 1778) and the Convergent Origin of Piscivory in the Atlantic and Indo-Pacific Cones

Abalde

Jiménez‐Tenorio

Afonso

et al. 2018

Genome Biology and Evolution

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The transcriptome of the venom duct of the Atlantic piscivorous cone species Chelyconus ermineus (Born, 1778) was determined. The venom repertoire of this species includes at least 378 conotoxin precursors, which could be ascribed to 33 known and 22 new (unassigned) protein superfamilies, respectively. Most abundant superfamilies were T, W, O1, M, O2, and Z, accounting for 57% of all detected diversity. A total of three individuals were sequenced showing considerable intraspecific variation: each individual had many exclusive conotoxin precursors, and only 20% of all inferred mature peptides were common to all individuals. Three different regions (distal, medium, and proximal with respect to the venom bulb) of the venom duct were analyzed independently. Diversity (in terms of number of distinct members) of conotoxin precursor superfamilies increased toward the distal region whereas transcripts detected toward the proximal region showed higher expression levels. Only the superfamilies A and I3 showed statistically significant differential expression across regions of the venom duct. Sequences belonging to the alpha (motor cabal) and kappa (lightning-strike cabal) subfamilies of the superfamily A were mainly detected in the proximal region of the venom duct. The mature peptides of the alpha subfamily had the α4/4 cysteine spacing pattern, which has been shown to selectively target muscle nicotinic-acetylcholine receptors, ultimately producing paralysis. This function is performed by mature peptides having a α3/5 cysteine spacing pattern in piscivorous cone species from the Indo-Pacific region, thereby supporting a convergent evolution of piscivory in cones.

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“…2004 ; Rivera-Ortiz et al. 2011 ; Echterbille et al. 2017 ) and some conotoxin precursors belonging to the A, B1, and O1 superfamilies ( Duda and Palumbi 2004 ; Gowd et al.…”

Section: Introductionmentioning

confidence: 99%

Conotoxin Diversity in Chelyconus ermineus (Born, 1778) and the Convergent Origin of Piscivory in the Atlantic and Indo-Pacific Cones

Abalde

Jiménez‐Tenorio

Afonso

et al. 2018

Genome Biology and Evolution

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“…Several novel α-conotoxins from other groups have also been discovered and investigated in recent years. Thus, in the venom of C. ermineus , two new 4/4-conotoxins, EIIA and EIIB, containing an increased number of post-translational modifications, were identified by mass spectrometry, and were shown to be highly selective blockers of the muscle-type nAChR from Torpedo [ 132 , 133 ]. Noteworthy, EIIA conotoxin distinguished two binding sites on this receptor ( Table 2 ).…”

Section: Marine Origin Peptides Targeting Nachrsmentioning

confidence: 99%

Marine Origin Ligands of Nicotinic Receptors: Low Molecular Compounds, Peptides and Proteins for Fundamental Research and Practical Applications

et al. 2022

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The purpose of our review is to briefly show what different compounds of marine origin, from low molecular weight ones to peptides and proteins, offer for understanding the structure and mechanism of action of nicotinic acetylcholine receptors (nAChRs) and for finding novel drugs to combat the diseases where nAChRs may be involved. The importance of the mentioned classes of ligands has changed with time; a protein from the marine snake venom was the first excellent tool to characterize the muscle-type nAChRs from the electric ray, while at present, muscle and α7 receptors are labeled with the radioactive or fluorescent derivatives prepared from α-bungarotoxin isolated from the many-banded krait. The most sophisticated instruments to distinguish muscle from neuronal nAChRs, and especially distinct subtypes within the latter, are α-conotoxins. Such information is crucial for fundamental studies on the nAChR revealing the properties of their orthosteric and allosteric binding sites and mechanisms of the channel opening and closure. Similar data are provided by low-molecular weight compounds of marine origin, but here the main purpose is drug design. In our review we tried to show what has been obtained in the last decade when the listed classes of compounds were used in the nAChR research, applying computer modeling, synthetic analogues and receptor mutants, X-ray and electron-microscopy analyses of complexes with the nAChRs, and their models which are acetylcholine-binding proteins and heterologously-expressed ligand-binding domains.

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“…Figs. values obtained by radioactive and non-radioactive ligand binding [24,36] indicating that the Torpedo-nAChRs were stable and functional under LFT conditions.…”

Section: Inhibition Of Toxin-tracer Binding To Torpedo-nachrs By α-Bu...mentioning

confidence: 99%

NeuroTorp, a lateral flow test based on toxin-receptor affinity for in-situ early detection of cyclic imine toxins

Noirmain

Dano

Hue

et al. 2022

Analytica Chimica Acta

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Discovery and characterization of EII B, a new α-conotoxin from Conus ermineus venom by nAChRs affinity capture monitored by MALDI-TOF/TOF mass spectrometry

Cited by 10 publications

References 42 publications

Conotoxin Diversity in Chelyconus ermineus (Born, 1778) and the Convergent Origin of Piscivory in the Atlantic and Indo-Pacific Cones

Conotoxin Diversity in Chelyconus ermineus (Born, 1778) and the Convergent Origin of Piscivory in the Atlantic and Indo-Pacific Cones

Marine Origin Ligands of Nicotinic Receptors: Low Molecular Compounds, Peptides and Proteins for Fundamental Research and Practical Applications

NeuroTorp, a lateral flow test based on toxin-receptor affinity for in-situ early detection of cyclic imine toxins

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