Discovering active sites in peptide Ala–Val–Thr–Phe that counter 2,2-azobis(2-methylpropanimidamidine)dihydrochloride-induced oxidative stress in HepG2 cells

Zhang

ACS Appl. Mater. Interfaces

et al. 2022

The residence time of some small molecular imaging and therapeutic agents in tumor tissue is short and the molecules can be easily dispersed, which decreases treatment efficacy. Therefore, methods that enhance oncotherapy performance are of significant importance. Here, we report an in situ self-assembly strategy aimed at enhancing the photothermal therapy of glioblastomas. The probe, ICG-PEP-c(RGD)fk, consisted of a glutathione-reactive self-assembling polypeptide as the skeleton, indocyanine green (ICG) as a theranostic agent, and cyclic Arg-Gly-Asp [c(RGD)fk] peptides as the targeting group. ICG-PEP-c(RGD)fk was synthesized and found to be assembled in the glutathione environment at 9.446 μM in vitro. Human glioblastoma cell line U87MG-luc with high integrin α v β 3 expression was applied to invivo experiments. ICG-PEP-c(RGD)fk provided clearer tumor imaging and had a tumor retention time of 6.12 times longer than that of ICG-c(RGD)fk. In therapeutic experiments, ICG-PEP-c(RGD)fk significantly suppressed glioblastoma growth and the tumor volume was 2.61 times smaller than in the ICG-c(RGD)fk group at the end of the observation period. Moreover, the median survival time of ICG-PEP-c(RGD)fk group was significantly improved by 2.78 times compared with that of the control group. In conclusion, glutathione-reactive self-assembling peptides are capable of increasing the tumor retention time and improving the photothermal therapeutic effect. The in situ self-assembly strategy is a potential and feasible method to enhance oncotherapy.

Section: Introductionmentioning

confidence: 99%

“…17 phobic parts and the hydrophobic part containing drugs selfassembles into nanostructures. 20,21 In this study, we designed a GSH-responsive Phe-Phe peptide named PEP as a self-assembled platform to enhance photothermal therapy (PTT) of glioma (Scheme. 1).…”

Section: ■ Introductionmentioning

confidence: 99%

Enhancing Photothermal Therapy Efficacy by In Situ Self-Assembly in Glioma

Zhang

ACS Appl. Mater. Interfaces

et al. 2022

“…9 For example, the potential mechanism of the tetrapeptide AVTF from edible Dendrobium aphyllum is the direct scavenging of free radicals without leading to the activation of the nuclear factor erythroid-2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway. 10 However, direct antioxidants are short-lived and easily depleted, and need to be replenished continuously during the antioxidant process. Indirect antioxidants activate oxidative stress-related pathways, such as the Keap1/Nrf2/ARE pathway, which can regulate the expression of related antioxidant proteins and thus protect cells.…”

Section: Introductionmentioning

confidence: 99%

Dual antioxidant activity and the related mechanisms of a novel pentapeptide GLP4 from the fermented mycelia of Ganoderma lingzhi

Huang

Luo

et al. 2022

Food Funct.

The Journal of Biochemistry

Formation of hierarchical assemblies by collagen peptides derived from fish skin and bladder and their subsequent application as antiperoxide agents in lipid-rich food

Sumeet

Bajaj

Kumar

et al. 2023

This study attempts to identify the significant role played by the secondary and tertiary structure of collagen-derived peptides that are involved in lipid peroxide quenching in food products. Fish collagen hydrolysate (CH) was extracted with an efficiency of 70%. The constituent peptides of CH (8.2-9.7kDa) existed in a polyproline-II (PP-II) conformation and at a minimum concentration of 1mg/ml and pH range 7-8, assembled into a stable, hierarchical, quasi-fibrillar (QF) network. The peroxide quenching activity of this QF-CH increased with increasing ionic stability of the assembly and decreased upon proteolytic dismantling. Upon being used as an additive, the QF-CH reduced peroxide formation by 84.5-98.9% in both plant and fish-based oil and increased the shelf life of soya oil by a factor of 5 after 6 months of storage. The addition of QF-CH to cultured cells quenched peroxide ions generated in situ and decreased stressor activity by a factor of 12. 16 abundant peptides were identified from the CH. The reason behind the high efficacy displayed by CH was attributed to its unique charge distribution, prevalence of proton-donating amino acid residues and proximal charge delocalization by the QF network, making fish derived CH a suitable substitute for antiperoxide agents in lipid-rich food.