Discordant influence of amphotericin B on epirubicin cytotoxicity in primary hepatic malignant cells collected by a new primary culture technique

Abstract

Amphotericin B has a discordant influence on epirubicin cytotoxicity in primary cultured hepatic malignant cells. Application of AmB in the systemic chemotherapy of primary hepatic malignancy should be limited to patients with positive AmB effect evaluated by an in vitro sensitivity test such as the present method.

Anti-cancer mechanisms of clinically acceptable colchicine concentrations on hepatocellular carcinoma

“…The remaining cell lines had been used in our previous studies (Lin et al, 2009(Lin et al, , 2012. The detailed methodology for the establishment of these cell lines was described in our previous study (Lin et al, 2006). The F59/KMUH CAF cell line was verified by positive stain for fibroblast activation protein (ENZO Life Sciences International, Inc., Butler Pike, Plymouth Meeting, PA, USA), α-smooth muscle actin (Sigma-Aldrich, St. Louis, Mo., USA) and CXCL12 (R & D Systems, Inc., Minneapolis, MN, USA).…”

Clinical utility of a simple primary culture method in hepatocellular carcinoma patients

“…If the tube contained visible specimens, then the patient was included for the study and the specimens were sent for primary culture. In comparison with our previous study, there was no extra session of FNA in this study to collect specimens solely for the purpose of primary culture due to ethical consideration and protection of the patients. The diagnosis of HCC was based on the cytological and/or histological results.…”

“…For patient with multiple tumors, the largest tumor was aspirated. The method for the collection of specimen for primary culture was similar to our previous study but with little modification . The specimens within the aspirated needle and syringe obtained from routine FNA were applied for both cytological and histological examinations at first.…”

“…The procedures for the plating of specimens into a 25‐cm 2 plastic culture flask for primary culture (37°C in a humidified atmosphere of 5% CO 2 and 95% air) were the same as our previous report, and were completed within 2 h of aspiration. The serum‐containing culture medium (10% fetal bovine serum, 90% Dulbecco's Modified Eagle's high glucose medium, supplemented with 20 mM l ‐glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin, HyClone, Logan, UT, USA) was replaced at the third day of the primary culture with 2‐mL new medium.…”

“…Ultrasound‐guided fine‐needle aspiration (FNA) cytology or biopsy of hepatic tumor is frequently applied in the collection of specimens for diagnosis because it is considered safe, efficacious, accurate, and cost‐effective . Our previous research from few patients showed that specimens obtained from FNA had potential to be applied for primary culture of cancer cells . This prospective study aimed to investigate the clinical utility of our primary culture method from a large number of HCC patients.…”