Discontinuous electrophoresis of β‐caseins for the determination of bovine caseins in milk and dairy products

Abstract

Discontinuous acidic anodic polyacrylamide gel electrophoresis enables the separation of bovine beta-caseins from those of ovine and caprine. Interfering protein bands as a consequence of ripening or processing have not been detected. After evaluation of the stained gel by laser densitometry, quantification was performed with calibration standards on the same gel by the ratio of the peak areas from bovine to ovine and bovine to caprine, respectively. Thus, independence from the extractability of proteins affected by denaturation and ripening (which might in some cases raise the limit of detection) is achieved. The range of quantification extends from 5 to approximately 70% bovine casein in relation to total casein.

Investigation on whey proteins profile of commercially available milk‐based probiotics health drinks using fast protein liquid chromatography (FPLC)

“…. electrophoresis using polyacrylamide gels (PAGE) with or without urea (Mayer and Hörtner, 1992;Carretero et al, 1994;Egito et al, 2002;Veloso et al, 2002) or sodium dodecylsulphate (SDS-PAGE) (Matar et al, 1996;Jin and Park, 1996;Tanabe et al, 2003) isoelectric focusing(IEF) (Kim and Jimenez-Flores, 1994), and capillary electrophoresis that shows extremely good resolution (Molina et al, 2000;Cartoni et al, 1999;Miralles et al, 2000); and…”

Forensics on wild animals: Differentiation between otter and pheasant blood using electrophoretic methods

“…Species identification by electrophoresis is routinely used in the food industry in order to prove adulterations, especially of meat [l], fish [2] or dairy products [3]. In forensic sciences, unknown blood samples are often [4, 51, but not necessarily identified by electrophoretic methods.…”